##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062414_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1171921
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12271902286929	32.0	32.0	32.0	32.0	32.0
2	30.861709108378466	32.0	32.0	32.0	32.0	32.0
3	30.845221648899543	32.0	32.0	32.0	32.0	32.0
4	30.88440432418226	32.0	32.0	32.0	32.0	32.0
5	30.750309961166323	32.0	32.0	32.0	32.0	32.0
6	34.36922966650482	36.0	36.0	36.0	32.0	36.0
7	34.22214637334769	36.0	36.0	36.0	32.0	36.0
8	34.23750918363951	36.0	36.0	36.0	32.0	36.0
9	34.3535451621739	36.0	36.0	36.0	32.0	36.0
10	34.0816539681429	36.0	36.0	36.0	32.0	36.0
11	34.35918291420668	36.0	36.0	36.0	32.0	36.0
12	34.162572391825044	36.0	36.0	36.0	32.0	36.0
13	34.276128681028844	36.0	36.0	36.0	32.0	36.0
14	34.18153527413537	36.0	36.0	36.0	32.0	36.0
15	34.15217152009393	36.0	36.0	36.0	32.0	36.0
16	34.14013743247198	36.0	36.0	36.0	32.0	36.0
17	34.06202124545938	36.0	36.0	36.0	32.0	36.0
18	34.096803453475104	36.0	36.0	36.0	32.0	36.0
19	34.082028566771996	36.0	36.0	36.0	32.0	36.0
20	34.06022419599956	36.0	36.0	36.0	32.0	36.0
21	34.01824440384633	36.0	36.0	36.0	32.0	36.0
22	33.9745964105089	36.0	36.0	36.0	32.0	36.0
23	33.9557649363737	36.0	36.0	36.0	32.0	36.0
24	33.93802995253093	36.0	36.0	36.0	32.0	36.0
25	33.47369745912907	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	51.0
5	115.0
6	193.0
7	46.0
8	187.0
9	195.0
10	137.0
11	46.0
12	87.0
13	59.0
14	221.0
15	226.0
16	413.0
17	554.0
18	790.0
19	1202.0
20	1820.0
21	2635.0
22	3800.0
23	5403.0
24	7247.0
25	9890.0
26	13550.0
27	17540.0
28	23327.0
29	31435.0
30	40592.0
31	54993.0
32	78838.0
33	114873.0
34	259286.0
35	502166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.741257350788906	17.42743936105846	11.55773148104689	25.273571807105743
2	15.997655046003082	20.385739812506078	37.27845390049135	26.33815124099949
3	19.727902186101044	24.22409541087743	27.908159521034122	28.13984288198741
4	12.554149868401737	15.42157528751916	35.42493778525758	36.599337058821526
5	14.402059558878136	36.541805340615085	33.34013365622063	15.716001444286148
6	34.10685946342334	34.77845532099072	17.167909208640342	13.9467760069456
7	29.795447520454392	30.65853671032206	20.868775946896278	18.677239822327266
8	28.223321467857314	32.370715276401825	20.10711381974953	19.298849435991336
9	27.082811970184718	15.02021251971927	19.069463934766475	38.82751157532954
10	16.16392014146954	26.665641525068985	31.10988663642499	26.060551697036487
11	37.050512747788225	21.672094286817547	21.71361301808713	19.56377994730709
12	24.56502645213326	24.048628863583545	28.70107722382469	22.6852674604585
13	29.461093498112405	19.509080020537585	25.647886397439805	25.381940083910205
14	23.742093230677753	19.979650000512596	25.057325145491387	31.220931623318265
15	25.29144090158536	27.003226415432806	23.443577024368533	24.2617556586133
16	25.691287344892803	25.801397865444777	24.646390613014056	23.860924176648368
17	23.42411919488774	25.90805795714725	26.21689505518915	24.450927792775857
18	24.927912156637593	24.285251702981853	27.79186071646132	22.994975423919236
19	24.81722451246342	26.038404852938037	25.70826836405579	23.43610227054275
20	24.887850427368683	24.138278650360732	26.654222214628355	24.319648707642234
21	26.244471082168452	24.839525749784055	24.520328731918077	24.39567443612941
22	24.83667447438876	25.092269842395677	25.48023472730085	24.59082095591471
23	23.412292874330088	24.660515558350653	25.91796661380291	26.00922495351635
24	23.85584868195648	26.13317225004251	25.66897554615523	24.342003521845783
25	24.545654163167207	24.80496300897371	25.50966790525444	25.13971492260464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	168.0
1	168.0
2	523.0
3	878.0
4	878.0
5	878.0
6	2509.0
7	4140.0
8	4140.0
9	4140.0
10	4141.5
11	4143.0
12	4143.0
13	4143.0
14	3813.0
15	3483.0
16	3483.0
17	3483.0
18	5797.0
19	8111.0
20	8111.0
21	8111.0
22	13216.0
23	18321.0
24	18321.0
25	18321.0
26	27527.0
27	36733.0
28	36733.0
29	36733.0
30	45797.0
31	54861.0
32	54861.0
33	54861.0
34	65106.0
35	75351.0
36	75351.0
37	75351.0
38	83506.0
39	91661.0
40	91661.0
41	91661.0
42	106190.5
43	120720.0
44	120720.0
45	120720.0
46	148020.5
47	175321.0
48	175321.0
49	175321.0
50	165724.0
51	156127.0
52	156127.0
53	156127.0
54	138839.5
55	121552.0
56	121552.0
57	121552.0
58	112257.0
59	102962.0
60	102962.0
61	102962.0
62	92058.0
63	81154.0
64	81154.0
65	81154.0
66	67243.5
67	53333.0
68	53333.0
69	53333.0
70	40652.0
71	27971.0
72	27971.0
73	27971.0
74	22019.0
75	16067.0
76	16067.0
77	16067.0
78	13528.0
79	10989.0
80	10989.0
81	10989.0
82	7696.0
83	4403.0
84	4403.0
85	4403.0
86	3258.5
87	2114.0
88	2114.0
89	2114.0
90	1410.5
91	707.0
92	707.0
93	707.0
94	423.0
95	139.0
96	139.0
97	139.0
98	325.5
99	512.0
100	512.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009215638255479677
2	0.0036691893054224644
3	0.005375788982363146
4	0.014762087205536891
5	0.034814633409589894
6	0.04539555140662212
7	0.06647205741683952
8	0.08678059357243363
9	0.09488694203790186
10	0.11528080817734303
11	0.1164754279512015
12	0.12842162568978624
13	0.11809669764429513
14	0.11988862730508286
15	0.10853973945342732
16	0.10913704934035655
17	0.11954730736969471
18	0.12577639619052822
19	0.12765365583516294
20	0.1182673576119892
21	0.12662969602899854
22	0.1460849323461223
23	0.13797858388065407
24	0.12901893557671548
25	0.12816563573824516
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1171921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.693610993580855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.68165981782064	38.31294135498221
2	13.03258668141246	12.692074122096425
3	3.797225894964376	5.547019217524418
4	1.5176968243399351	2.956085550824073
5	0.7724396220549696	1.8806437236186655
6	0.4605404035117922	1.3455225153257981
7	0.28874245616290545	0.9841938986409315
8	0.20696802188749572	0.8062416276720515
9	0.1472542007917535	0.6453304893471862
>10	0.8489263810291667	7.842448297364182
>50	0.10461759547915567	3.640646787995831
>100	0.11954307091124652	12.384741971174261
>500	0.014767084590864244	4.655759599536843
>1k	0.006680347791105255	5.2168852922441316
>5k	3.5159725216343444E-4	1.0894655516530376
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6622	0.5650551530350595	No Hit
TCCATGTACTCTGCGTTGATACCAC	6105	0.5209395513861429	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4082	0.348316994063593	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3052	0.26042711070114793	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2975	0.2538567019449263	No Hit
CATGTACTCTGCGTTGATACCACTG	2851	0.2432757839478941	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2731	0.23303618588625002	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2183	0.18627535473807535	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1876	0.1600790496970359	No Hit
CCCATGTACTCTGCGTTGATACCAC	1870	0.1595670697939537	No Hit
GCGTTGATACCACTGCTTCCATGTA	1825	0.15572722052083715	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1805	0.15402062084389648	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1672	0.14267173299224092	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1626	0.1387465537352774	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1567	0.13371208468830237	No Hit
GTATCAACGCAGAGTACATGGAAGC	1548	0.13209081499520872	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1536	0.13106685518904432	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1513	0.12910426556056254	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1472	0.12560573622283414	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1385	0.11818202762814217	No Hit
GAACTACGACGGTATCTGATCGTCT	1369	0.11681674788658963	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1355	0.11562212811273116	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1284	0.10956369925959172	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1275	0.10879572940496841	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1254	0.10700379974418071	No Hit
TCGTAGTTCCGACCATAAACGATGC	1226	0.10461456019646374	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1221	0.10418791027722858	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1208	0.10307862048721714	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1207	0.10299329050337011	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1193	0.10179867072951164	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTT	25	0.006029094	19.001152	12
CGCATCG	190	0.0	13.499667	13
GCTCGTA	85	2.6936868E-7	13.413153	9
CGGCTAA	65	5.4449592E-5	13.155207	9
CGGTCCA	310	0.0	12.872299	10
GTCCTAG	75	1.483423E-5	12.662026	1
GGTCCAA	355	0.0	12.042984	11
ATTACAC	95	1.0436415E-6	11.9956045	3
TCGCGTA	135	3.6925485E-10	11.964201	9
AATCGCT	80	2.8656703E-5	11.874705	15
GATCTAC	80	2.8757093E-5	11.870649	1
CGCCAGT	210	0.0	11.766641	18
AAGCTCG	105	2.7153146E-7	11.762619	7
GCATCGC	220	0.0	11.658802	14
AGAATTT	335	0.0	11.630056	17
CGGACAT	180	0.0	11.60884	5
CAAGACG	295	0.0	11.590458	4
GTCCTAT	295	0.0	11.588973	1
TTTGCGC	205	0.0	11.588545	17
GTCTTGA	115	7.10188E-8	11.560981	1
>>END_MODULE