FastQCFastQC Report
Thu 2 Feb 2017
SRR4062414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171921
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41180.35138887348208625No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32970.2813329567436713No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16090.1372959440098778No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16080.13721061402603077No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA15640.13345609473676126No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15150.12927492552825662No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC15110.12893360559286846No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14330.1222778668527998No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14080.12014461725662397No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA13550.11562212811273116No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG13130.11203826879115572No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC12920.11024633913036799No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12420.10597983993801631No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12160.1037612603579934No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12140.10359060039029934No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11960.10205466068105273No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11890.1014573507941235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT407.0322404E-427.49984242
GTATAGA1650.025.339671
GTAATAC803.6155689E-621.9998723
AATACCG500.002580479321.999875
TAATACC1259.968062E-1021.1198774
GAACGAT654.9322326E-420.3075756
GATATAC3850.019.4334341
AGGACGT3850.019.428465
CGTCTTA1404.081812E-918.85703315
TCCTACA4900.018.8570332
TAGGACG4000.018.699894
CGCAATA4550.018.37351836
TGTAGGA6250.018.3038922
ATACACT4350.018.206794
CGGTCCA4600.018.1738110
ACGATTA851.4308495E-418.11754232
TCTATAC851.4308495E-418.1175423
GGACGTG4050.017.9258216
ACGACGG4050.017.9258216
AACGAAT2850.017.75428231
Produced by FastQC (version 0.11.5)