##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1171921
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246727381794507	32.0	32.0	32.0	32.0	32.0
2	31.43601659156206	32.0	32.0	32.0	32.0	32.0
3	31.505675723875587	32.0	32.0	32.0	32.0	32.0
4	31.608113516184112	32.0	32.0	32.0	32.0	32.0
5	31.545256036883032	32.0	32.0	32.0	32.0	32.0
6	35.1420718632058	36.0	36.0	36.0	36.0	36.0
7	35.14413599551506	36.0	36.0	36.0	36.0	36.0
8	35.09805268443863	36.0	36.0	36.0	36.0	36.0
9	35.19632552023558	36.0	36.0	36.0	36.0	36.0
10	35.09829331499308	36.0	36.0	36.0	36.0	36.0
11	35.20997831765111	36.0	36.0	36.0	36.0	36.0
12	35.11836036729438	36.0	36.0	36.0	36.0	36.0
13	35.16191535094942	36.0	36.0	36.0	36.0	36.0
14	35.12218571047025	36.0	36.0	36.0	36.0	36.0
15	35.09444919922077	36.0	36.0	36.0	36.0	36.0
16	35.10504632991473	36.0	36.0	36.0	36.0	36.0
17	35.07128381520597	36.0	36.0	36.0	36.0	36.0
18	35.09041565088432	36.0	36.0	36.0	36.0	36.0
19	35.054563404871146	36.0	36.0	36.0	36.0	36.0
20	35.03456376325708	36.0	36.0	36.0	36.0	36.0
21	35.014918241075975	36.0	36.0	36.0	36.0	36.0
22	35.01056299870042	36.0	36.0	36.0	36.0	36.0
23	34.95928991800642	36.0	36.0	36.0	32.0	36.0
24	34.94016576202662	36.0	36.0	36.0	32.0	36.0
25	34.91056479063008	36.0	36.0	36.0	32.0	36.0
26	34.87148536462782	36.0	36.0	36.0	32.0	36.0
27	34.85976529134643	36.0	36.0	36.0	32.0	36.0
28	34.837018877552325	36.0	36.0	36.0	32.0	36.0
29	34.80816198361494	36.0	36.0	36.0	32.0	36.0
30	34.78733464115755	36.0	36.0	36.0	32.0	36.0
31	34.78343164769639	36.0	36.0	36.0	32.0	36.0
32	34.73912576018349	36.0	36.0	36.0	32.0	36.0
33	34.69844554368426	36.0	36.0	36.0	32.0	36.0
34	34.67195143699959	36.0	36.0	36.0	32.0	36.0
35	34.62688952582981	36.0	36.0	36.0	32.0	36.0
36	34.59420899531624	36.0	36.0	36.0	32.0	36.0
37	34.572511287023616	36.0	36.0	36.0	32.0	36.0
38	34.51007619114257	36.0	36.0	36.0	32.0	36.0
39	34.470620459911544	36.0	36.0	36.0	32.0	36.0
40	34.430319108540594	36.0	36.0	36.0	32.0	36.0
41	34.412673721180866	36.0	36.0	36.0	32.0	36.0
42	34.324758238823264	36.0	36.0	36.0	32.0	36.0
43	34.32519086184137	36.0	36.0	36.0	32.0	36.0
44	34.29139677503859	36.0	36.0	36.0	32.0	36.0
45	34.188662887686114	36.0	36.0	36.0	32.0	36.0
46	34.22571146007282	36.0	36.0	36.0	32.0	36.0
47	34.166134065350825	36.0	36.0	36.0	32.0	36.0
48	34.11280794524546	36.0	36.0	36.0	32.0	36.0
49	34.109337574802396	36.0	36.0	36.0	32.0	36.0
50	33.56888902920931	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	4.0
21	20.0
22	101.0
23	281.0
24	835.0
25	1981.0
26	4153.0
27	7793.0
28	13663.0
29	20813.0
30	29995.0
31	42612.0
32	61961.0
33	94471.0
34	197702.0
35	695533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.349096594531346	18.002394743512387	12.094905580025483	25.55360308193078
2	15.594167973690004	20.844903120983997	37.039135606729054	26.521793298596947
3	19.158386060422472	24.692319835482948	28.17832351322408	27.970970590870497
4	12.356325271904689	15.873124228828297	35.57637991736623	36.19417058190078
5	14.180831456072088	36.91258788995836	33.053792067717936	15.85278858625162
6	34.267019593587925	34.94761561001917	16.912162496565447	13.873202299827462
7	29.970364896609926	30.604196016625696	20.767440808723457	18.657998278040928
8	28.522656390661144	32.47164271311803	19.821984587698317	19.183716308522502
9	27.337593574993534	14.73973074976897	18.913988229582028	39.00868744565547
10	16.28292350764258	26.74258759762817	30.88911283269094	26.085376062038314
11	37.389721662125694	21.512712887643453	21.656749900377246	19.440815549853614
12	24.6630105612921	24.047440057819596	28.495777445749333	22.793771935138974
13	29.76429298561934	19.479384702552476	25.528683247420258	25.227639064407924
14	23.99624206751138	19.967301550189816	24.82931869980997	31.20713768248884
15	25.336605453780585	27.158912588817845	23.22392038371187	24.280561573689692
16	25.83305529980263	25.816330622968614	24.4385927037744	23.912021373454355
17	23.67992381739042	25.94961605773768	25.900721976993328	24.46973814787857
18	25.140858470835493	24.298054220378337	27.498781914480585	23.06230539430559
19	25.03103878162436	26.005592527141335	25.535168326192636	23.428200365041672
20	25.14751420957556	24.01893984321469	26.50076242340567	24.332783523804082
21	26.565442551161727	24.731615868305116	24.327919714724796	24.37502186580836
22	25.06525610514702	24.98897109958777	25.340018653134464	24.60575414213074
23	23.618144909085167	24.57921651715431	25.726222160026147	26.07641641373437
24	23.9725203320019	26.109439117483173	25.57851595798693	24.339524592527994
25	24.952279206533547	24.558651991047178	25.317235547447307	25.171833254971965
26	23.581538346014792	25.644902685419922	26.399134412643853	24.374424555921433
27	25.294537771744004	25.176441074099703	25.588755556048575	23.94026559810772
28	24.00503105584762	24.569574228979597	26.27933111532262	25.14606359985016
29	23.55337945134527	25.3663856181432	26.648895275364126	24.4313396551474
30	24.32672509495094	25.26654953704217	26.622357650387695	23.78436771761919
31	24.172704474107043	25.292404522147827	25.266464207058327	25.268426796686803
32	23.902635075231178	25.552490312913584	25.39676309239275	25.14811151946249
33	23.49347780268465	24.97139312291528	26.15099481961668	25.384134254783387
34	24.123810393362692	25.532352436725684	26.50110374334106	23.84273342657056
35	25.46775763895348	25.12123257455067	25.925297012341275	23.48571277415457
36	23.83121387875121	26.032386142069303	25.615207851041156	24.52119212813833
37	25.108603736941316	25.72144368093071	25.595923274691724	23.574029307436252
38	24.177056303283244	25.272181315976077	25.464941749486524	25.08582063125415
39	24.48117236571407	24.898179996774527	25.701135144775115	24.919512492736285
40	25.137871921400844	25.383792934848	25.440708034073968	24.03762710967719
41	23.31061564730046	25.649766494499204	26.684819198563726	24.354798659636614
42	25.78185730949441	25.93638991024139	25.51980892910017	22.76194385116403
43	24.470676777700888	24.29276376137982	25.725027540252288	25.511531920667007
44	23.78240512799071	25.322355346478133	26.187516052703213	24.707723472827947
45	24.214857486127478	25.654971623513873	25.723918250462276	24.406252639896376
46	23.590347463990717	24.9655266571097	26.427742508021023	25.016383370878557
47	24.29191046154135	24.79885589557658	26.70538372467086	24.20384991821121
48	24.651917663391988	26.601963784248255	24.74074617657675	24.00537237578301
49	23.442194482392583	25.66051807246393	26.402035632094655	24.49525181304883
50	23.447163628916652	26.855160761826745	24.706720595262475	24.990955013994128
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	21.0
2	28.0
3	104.0
4	180.0
5	208.0
6	236.0
7	232.5
8	229.0
9	302.5
10	376.0
11	586.5
12	797.0
13	1423.5
14	2050.0
15	2829.0
16	3608.0
17	4251.5
18	4895.0
19	5330.5
20	5766.0
21	6503.0
22	7240.0
23	7730.5
24	8221.0
25	8423.5
26	8626.0
27	10833.0
28	13040.0
29	15284.5
30	17529.0
31	19383.0
32	21237.0
33	24239.5
34	27242.0
35	30981.0
36	34720.0
37	40006.0
38	45292.0
39	48257.5
40	51223.0
41	55192.5
42	59162.0
43	60844.5
44	62527.0
45	72208.5
46	81890.0
47	90221.5
48	98553.0
49	97708.0
50	96863.0
51	87432.5
52	78002.0
53	74890.5
54	71779.0
55	74023.5
56	76268.0
57	74166.0
58	72064.0
59	65831.0
60	59598.0
61	52403.5
62	45209.0
63	39564.0
64	33919.0
65	28960.0
66	24001.0
67	21045.0
68	18089.0
69	16945.5
70	15802.0
71	12199.5
72	8597.0
73	7781.5
74	6966.0
75	4904.5
76	2843.0
77	2658.5
78	2474.0
79	2139.5
80	1805.0
81	1482.0
82	1159.0
83	1000.5
84	842.0
85	706.5
86	571.0
87	427.0
88	283.0
89	172.0
90	61.0
91	41.5
92	22.0
93	16.5
94	11.0
95	6.0
96	1.0
97	4.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0160420369632424
2	0.0040105092408106
3	5.119799030822044E-4
4	2.559899515411022E-4
5	8.532998384703406E-5
6	5.973098869292385E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.532998384703406E-5
47	0.0
48	0.0
49	0.0
50	8.532998384703406E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1171921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.07620267954002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.37633742825115	42.129945733014175
2	12.837739203897497	13.627568958662806
3	3.6503333500809587	5.812374982103438
4	1.39284407433718	2.957074975620659
5	0.7087464359226833	1.880878474071698
6	0.4253331102654385	1.3545039820060536
7	0.27573088572588295	1.0244323863057236
8	0.20443644703422487	0.8680568238298855
9	0.14045384488906362	0.6709281064607355
>10	0.7764123472126131	7.755893673790492
>50	0.09409437065887052	3.5027461457068982
>100	0.10232002004224199	11.395912303420637
>500	0.011818608114877827	4.243724365094887
>1k	0.0033998735672936212	2.7759590899119253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4118	0.35138887348208625	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3297	0.2813329567436713	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1609	0.1372959440098778	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1608	0.13721061402603077	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1564	0.13345609473676126	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1515	0.12927492552825662	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1511	0.12893360559286846	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1433	0.1222778668527998	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1408	0.12014461725662397	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1355	0.11562212811273116	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1313	0.11203826879115572	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1292	0.11024633913036799	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1242	0.10597983993801631	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1216	0.1037612603579934	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1214	0.10359060039029934	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1196	0.10205466068105273	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1189	0.1014573507941235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	8.532998384703406E-5
10	0.0	0.0	0.0	0.0	8.532998384703406E-5
11	0.0	0.0	0.0	0.0	8.532998384703406E-5
12	0.0	0.0	0.0	0.0	8.532998384703406E-5
13	0.0	0.0	0.0	0.0	8.532998384703406E-5
14	0.0	0.0	0.0	0.0	8.532998384703406E-5
15	0.0	0.0	0.0	0.0	1.706599676940681E-4
16	0.0	0.0	0.0	0.0	1.706599676940681E-4
17	0.0	0.0	0.0	0.0	1.706599676940681E-4
18	0.0	0.0	0.0	8.532998384703406E-5	1.706599676940681E-4
19	0.0	0.0	0.0	8.532998384703406E-5	1.706599676940681E-4
20	0.0	0.0	0.0	5.119799030822044E-4	1.706599676940681E-4
21	0.0	0.0	0.0	0.0010239598061644087	1.706599676940681E-4
22	0.0	0.0	0.0	0.001365279741552545	2.559899515411022E-4
23	0.0	0.0	0.0	0.0026452294992580557	2.559899515411022E-4
24	0.0	0.0	0.0	0.004522489143892805	2.559899515411022E-4
25	0.0	0.0	0.0	0.005973098869292384	2.559899515411022E-4
26	0.0	0.0	0.0	0.0080210184816212	2.559899515411022E-4
27	0.0	0.0	0.0	0.01049558801318519	2.559899515411022E-4
28	0.0	0.0	0.0	0.018431276510959358	2.559899515411022E-4
29	0.0	0.0	0.0	0.03071879418493226	2.559899515411022E-4
30	0.0	0.0	0.0	0.05580580943596027	2.559899515411022E-4
31	0.0	0.0	0.0	0.10751577964726292	2.559899515411022E-4
32	0.0	0.0	0.0	0.17031864775867997	2.559899515411022E-4
33	0.0	0.0	0.0	0.2315002461770034	2.559899515411022E-4
34	0.0	0.0	0.0	0.30633464201085225	2.559899515411022E-4
35	0.0	0.0	0.0	0.3891047263424753	2.559899515411022E-4
36	0.0	0.0	0.0	0.5203422414992137	2.559899515411022E-4
37	0.0	0.0	0.0	0.7078975459949945	2.559899515411022E-4
38	0.0	0.0	0.0	0.9381178424142924	2.559899515411022E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGT	40	7.0322404E-4	27.499842	42
GTATAGA	165	0.0	25.33967	1
GTAATAC	80	3.6155689E-6	21.999872	3
AATACCG	50	0.0025804793	21.99987	5
TAATACC	125	9.968062E-10	21.119877	4
GAACGAT	65	4.9322326E-4	20.307575	6
GATATAC	385	0.0	19.433434	1
AGGACGT	385	0.0	19.42846	5
CGTCTTA	140	4.081812E-9	18.857033	15
TCCTACA	490	0.0	18.857033	2
TAGGACG	400	0.0	18.69989	4
CGCAATA	455	0.0	18.373518	36
TGTAGGA	625	0.0	18.303892	2
ATACACT	435	0.0	18.20679	4
CGGTCCA	460	0.0	18.17381	10
ACGATTA	85	1.4308495E-4	18.117542	32
TCTATAC	85	1.4308495E-4	18.117542	3
GGACGTG	405	0.0	17.925821	6
ACGACGG	405	0.0	17.925821	6
AACGAAT	285	0.0	17.754282	31
>>END_MODULE