FastQCFastQC Report
Thu 2 Feb 2017
SRR4062413_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062413_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554176
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG261034.710236459175424No Hit
TCCATGTACTCTGCGTTGATACCAC217423.923302344381568No Hit
GAGTACATGGAAGCAGTGGTATCAA110831.9999061669938791No Hit
CATGTACTCTGCGTTGATACCACTG94951.713354602148054No Hit
GCTTCCATGTACTCTGCGTTGATAC67081.2104457789583092No Hit
CCCATGTACTCTGCGTTGATACCAC64521.1642510682526852No Hit
GTATCAACGCAGAGTACTTTTTTTT60941.0996506525002887No Hit
GTACATGGGAAGCAGTGGTATCAAC60921.099289756322901No Hit
GCGTTGATACCACTGCTTCCATGTA60101.0844930130500057No Hit
GTACTTTTTTTTTTTTTTTTTTTTT57071.0298172421757708No Hit
CATGGAAGCAGTGGTATCAACGCAG56001.0105092966855296No Hit
GTATCAACGCAGAGTACATGGAAGC46610.8410685414020095No Hit
TATCAACGCAGAGTACTTTTTTTTT44000.7939715902529161No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40540.7315365515648458No Hit
ACTCTGCGTTGATACCACTGCTTCC40080.7232359394849289No Hit
ACGCAGAGTACATGGAAGCAGTGGT37240.671988682295877No Hit
CAGTGGTATCAACGCAGAGTACATG36740.6629662778611849No Hit
TATCAACGCAGAGTACATGGAAGCA30090.5429682988797783No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29090.5249234900103937No Hit
GTATCAACGCAGAGTACATGGGAAG28010.5054350964314586No Hit
GTACATGGGGTGGTATCAACGCAAA23180.41827866959233173No Hit
ATACCACTGCTTCCATGTACTCTGC21540.3886851830465412No Hit
GGTATCAACGCAGAGTACTTTTTTT21250.3834521884744197No Hit
GGTATCAACGCAGAGTACATGGAAG20860.3764147130153597No Hit
GAGTACATGGGAAGCAGTGGTATCA20660.37280575124148285No Hit
AAGCAGTGGTATCAACGCAGAGTAC19540.35259556530777225No Hit
GCGTTGATACCACTGCTTCCCATGT18870.3405055433652847No Hit
GTACTCTGCGTTGATACCACTGCTT17400.3139796743272895No Hit
GTGGTATCAACGCAGAGTACATGGA17180.310009816376025No Hit
TATCAACGCAGAGTACATGGGAAGC17120.3089271278438619No Hit
CCACTGCTTCCATGTACTCTGCGTT16730.3018896523848019No Hit
GAAGCAGTGGTATCAACGCAGAGTA15580.28113812218500983No Hit
AAAAAGTACTCTGCGTTGATACCAC14990.270491684952073No Hit
GTACATGGGTGGTATCAACGCAAAA14500.2616497286060746No Hit
ACATGGAAGCAGTGGTATCAACGCA14280.25767987065481No Hit
GGTATCAACGCAGAGTACATGGGAA14210.25641673403395315No Hit
GCTTCCCATGTACTCTGCGTTGATA14140.2551535974130962No Hit
CTGCTTCCATGTACTCTGCGTTGAT13760.2482965700427301No Hit
ACGCAGAGTACATGGGAAGCAGTGG13430.24234178311583324No Hit
GTTGATACCACTGCTTCCATGTACT13330.24053730222889477No Hit
TGGAAGCAGTGGTATCAACGCAGAG12760.23025176117334564No Hit
GTACATGGGAGTGGTATCAACGCAA12750.23007131308465179No Hit
GCAGAGTACTTTTTTTTTTTTTTTT12430.2242969742464488No Hit
GATACCACTGCTTCCATGTACTCTG11140.20101917080494283No Hit
GCAGAGTACATGGAAGCAGTGGTAT10390.1874855641529045No Hit
CCCCATGTACTCTGCGTTGATACCA10250.18495929091119068No Hit
GTATCAACGCAGAGTACATGGGGTG9680.17467374985564152No Hit
GCAGTGGTATCAACGCAGAGTACAT9490.17124523617045848No Hit
CTTCCATGTACTCTGCGTTGATACC9340.16853851484005083No Hit
CCATGTACTCTGCGTTGATACCACT8810.15897476613927705No Hit
GTGGTATCAACGCAGAGTACATGGG7760.14002771682642337No Hit
ATCAACGCAGAGTACATGGAAGCAG7530.13587741078646495No Hit
TATCAACGCAGAGTACATGGGGTGG7240.13064441621434347No Hit
ATCAACGCAGAGTACTTTTTTTTTT7230.13046396812564962No Hit
GTCCTACAGTGGACATTTCTAAATT6870.12396783693267122No Hit
TGATACCACTGCTTCCATGTACTCT6790.12252425222312045No Hit
CTGCTTCCCATGTACTCTGCGTTGA6790.12252425222312045No Hit
CACTGCTTCCATGTACTCTGCGTTG6760.12198290795703891No Hit
AAAGTACTCTGCGTTGATACCACTG6700.12090021942487586No Hit
GTCCTAAAGTGTGTATTTCTCATTT6290.11350184778842823No Hit
GTATCAACGCAGAGTACATGGGAGT6230.11241915925626515No Hit
CTGTAGGACGTGGAATATGGCAAGA5820.10502078761981752No Hit
CTTTAGGACGTGAAATATGGCGAGG5550.10014868922508373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCC307.7070063E-419.00261915
CGCATCG250.00602562819.00090213
AAGTGTC307.711689E-419.0009027
GCGTTAT350.002173684516.2791391
CGTTATT350.002173684516.2791392
TCCAACG551.1232622E-515.54900218
CGCCAGT456.7409396E-414.78114918
CTATGGA456.7594287E-414.7758114
ATCCCGT653.361003E-614.61607910
ACGGTAT653.361003E-614.6160799
ATCGCCA400.005268606414.25196416
TTTGCTT400.005271764514.25067810
TAGAGGC400.00527808514.2481045
TAGCCCT400.00527808514.2481044
TTTAGGC400.00528124714.2468193
CCAACGA551.9527852E-413.82133519
GGTATCT707.2388157E-613.57207411
AGAACAG1301.4551915E-1113.1520975
ATACCAA1450.013.10643719
AAAAAGT1750.013.0233111
Produced by FastQC (version 0.11.5)