##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062413_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	554176
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.703435370712555	32.0	32.0	32.0	32.0	32.0
2	30.755754489548448	32.0	32.0	32.0	32.0	32.0
3	30.76328458828964	32.0	32.0	32.0	32.0	32.0
4	30.663572583439198	32.0	32.0	32.0	32.0	32.0
5	30.728613292528006	32.0	32.0	32.0	32.0	32.0
6	34.22172198002079	36.0	36.0	36.0	32.0	36.0
7	33.93740797147477	36.0	36.0	36.0	32.0	36.0
8	33.9232698637256	36.0	36.0	36.0	32.0	36.0
9	33.91340115775494	36.0	36.0	36.0	32.0	36.0
10	33.89928289929553	36.0	36.0	36.0	32.0	36.0
11	34.147996304423145	36.0	36.0	36.0	32.0	36.0
12	34.00276987816145	36.0	36.0	36.0	32.0	36.0
13	34.01833533029218	36.0	36.0	36.0	32.0	36.0
14	33.95369702044116	36.0	36.0	36.0	32.0	36.0
15	34.00763475863263	36.0	36.0	36.0	32.0	36.0
16	33.90798049717058	36.0	36.0	36.0	32.0	36.0
17	33.83703011317704	36.0	36.0	36.0	32.0	36.0
18	33.87703364995958	36.0	36.0	36.0	32.0	36.0
19	33.831223293682875	36.0	36.0	36.0	32.0	36.0
20	33.76443404261462	36.0	36.0	36.0	32.0	36.0
21	33.550231334449705	36.0	36.0	36.0	27.0	36.0
22	33.508749927820766	36.0	36.0	36.0	27.0	36.0
23	33.34927712495669	36.0	36.0	36.0	21.0	36.0
24	33.371520960849985	36.0	36.0	36.0	21.0	36.0
25	32.68770029737845	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	21.0
5	56.0
6	85.0
7	23.0
8	84.0
9	125.0
10	65.0
11	15.0
12	36.0
13	30.0
14	244.0
15	294.0
16	440.0
17	633.0
18	919.0
19	1251.0
20	1796.0
21	2440.0
22	3177.0
23	3989.0
24	4751.0
25	5805.0
26	7237.0
27	8793.0
28	11400.0
29	15018.0
30	19749.0
31	27961.0
32	40253.0
33	57092.0
34	119373.0
35	221016.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.74975186328118	16.114088751736958	13.935719055095374	21.20044032988649
2	11.100564466067071	23.792750003609118	38.232650969409114	26.874034560914694
3	20.9964053183964	31.055558763660514	26.51185956199743	21.436176355945662
4	12.069273569599327	18.282310009330562	33.17295111109507	36.47546530997504
5	11.158965620566287	40.350798023083286	33.43718524027336	15.053051116077071
6	31.04934215277076	31.42484221776115	21.321002007481116	16.204813621986975
7	25.58582146823283	33.003367672153054	23.328126833936746	18.08268402567737
8	30.618794848222684	29.646379722327076	23.44398227834841	16.290843151101825
9	28.110239491646965	17.52611396399407	18.363494818751118	36.00015172560785
10	16.385920032374386	29.415165376749698	32.24304637197303	21.955868218902886
11	33.63462268071037	23.993785116800055	18.91022745749851	23.461364744991055
12	21.885197619245517	26.68266364073302	30.212056273692806	21.22008246632866
13	33.487386379855785	21.877580222913192	24.667156890200168	19.967876507030855
14	26.567849664216116	19.560743729233703	25.097338481357085	28.774068125193097
15	26.978996925233677	24.250367635103643	31.45059598437686	17.320039455285816
16	25.241848508442793	25.347710862091706	29.6772282128592	19.7332124166063
17	22.656768031914606	24.820543581756784	31.77385871499629	20.748829671332324
18	25.998890573306184	20.730017219603244	35.17135583144665	18.09973637564393
19	20.94730964998979	33.720306229570994	27.212837502100495	18.119546618338724
20	21.44630294095329	21.39318583399278	38.04919294518098	19.11131827987295
21	22.670865811660036	28.531238820047484	23.613143829750328	25.184751538542155
22	20.195148928018273	29.89349762615191	26.696799868430805	23.214553577399013
23	19.04381562992987	28.501211629474493	25.928328638005592	26.52664410259005
24	18.273265079703517	32.82390057568672	26.97379595465338	21.929038389956382
25	25.35212285586769	25.265752246448958	24.31188101816132	25.070243879522035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	328.0
1	328.0
2	843.5
3	1359.0
4	1359.0
5	1359.0
6	4070.5
7	6782.0
8	6782.0
9	6782.0
10	6223.5
11	5665.0
12	5665.0
13	5665.0
14	3865.0
15	2065.0
16	2065.0
17	2065.0
18	3373.5
19	4682.0
20	4682.0
21	4682.0
22	7541.0
23	10400.0
24	10400.0
25	10400.0
26	15472.5
27	20545.0
28	20545.0
29	20545.0
30	24795.0
31	29045.0
32	29045.0
33	29045.0
34	29459.0
35	29873.0
36	29873.0
37	29873.0
38	32058.0
39	34243.0
40	34243.0
41	34243.0
42	48626.0
43	63009.0
44	63009.0
45	63009.0
46	117045.0
47	171081.0
48	171081.0
49	171081.0
50	125111.0
51	79141.0
52	79141.0
53	79141.0
54	53772.0
55	28403.0
56	28403.0
57	28403.0
58	24601.0
59	20799.0
60	20799.0
61	20799.0
62	18920.0
63	17041.0
64	17041.0
65	17041.0
66	14800.5
67	12560.0
68	12560.0
69	12560.0
70	9874.0
71	7188.0
72	7188.0
73	7188.0
74	6068.0
75	4948.0
76	4948.0
77	4948.0
78	3811.0
79	2674.0
80	2674.0
81	2674.0
82	1975.0
83	1276.0
84	1276.0
85	1276.0
86	896.5
87	517.0
88	517.0
89	517.0
90	355.0
91	193.0
92	193.0
93	193.0
94	116.5
95	40.0
96	40.0
97	40.0
98	179.5
99	319.0
100	319.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00830061207991685
2	0.004330754128652269
3	0.004330754128652269
4	0.0149771913615891
5	0.03284155214227971
6	0.04475112599607345
7	0.06875072179235478
8	0.0907653886130038
9	0.09834420833814528
10	0.11765215382838665
11	0.12017842707010049
12	0.13317068945605728
13	0.12396783693267122
14	0.12541142164222197
15	0.11512588058667282
16	0.11314095161104054
17	0.12613321399699734
18	0.13299024136736343
19	0.13262934518997574
20	0.12306559648920198
21	0.1317271047465065
22	0.1542831158332371
23	0.14237354197944335
24	0.1353360665203834
25	0.1347947222543019
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	554176.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.791536696133946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.60307154924833	20.255224657521264
2	12.890804717742247	6.907289352761758
3	4.121488367372724	3.3126302051146648
4	1.889928998822615	2.025364085001751
5	1.0486900889268185	1.4048009500177412
6	0.6844354425438802	1.1002246365029424
7	0.5019403480729147	0.9413427278266017
8	0.38821570207358697	0.8320716182495925
9	0.2950892232510247	0.7115304378027142
>10	2.069158124218049	11.066342135870556
>50	0.26672279593655557	5.057899282689564
>100	0.19667438488251066	10.452703127110956
>500	0.012797305865642816	2.4490994707147156
>1k	0.02357398448934203	13.437746115952898
>5k	0.005388339311849607	9.412034349656508
>10k+	0.0020206272419436024	10.633696847205773
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	26103	4.710236459175424	No Hit
TCCATGTACTCTGCGTTGATACCAC	21742	3.923302344381568	No Hit
GAGTACATGGAAGCAGTGGTATCAA	11083	1.9999061669938791	No Hit
CATGTACTCTGCGTTGATACCACTG	9495	1.713354602148054	No Hit
GCTTCCATGTACTCTGCGTTGATAC	6708	1.2104457789583092	No Hit
CCCATGTACTCTGCGTTGATACCAC	6452	1.1642510682526852	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6094	1.0996506525002887	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6092	1.099289756322901	No Hit
GCGTTGATACCACTGCTTCCATGTA	6010	1.0844930130500057	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5707	1.0298172421757708	No Hit
CATGGAAGCAGTGGTATCAACGCAG	5600	1.0105092966855296	No Hit
GTATCAACGCAGAGTACATGGAAGC	4661	0.8410685414020095	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4400	0.7939715902529161	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4054	0.7315365515648458	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4008	0.7232359394849289	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	3724	0.671988682295877	No Hit
CAGTGGTATCAACGCAGAGTACATG	3674	0.6629662778611849	No Hit
TATCAACGCAGAGTACATGGAAGCA	3009	0.5429682988797783	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2909	0.5249234900103937	No Hit
GTATCAACGCAGAGTACATGGGAAG	2801	0.5054350964314586	No Hit
GTACATGGGGTGGTATCAACGCAAA	2318	0.41827866959233173	No Hit
ATACCACTGCTTCCATGTACTCTGC	2154	0.3886851830465412	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2125	0.3834521884744197	No Hit
GGTATCAACGCAGAGTACATGGAAG	2086	0.3764147130153597	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2066	0.37280575124148285	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1954	0.35259556530777225	No Hit
GCGTTGATACCACTGCTTCCCATGT	1887	0.3405055433652847	No Hit
GTACTCTGCGTTGATACCACTGCTT	1740	0.3139796743272895	No Hit
GTGGTATCAACGCAGAGTACATGGA	1718	0.310009816376025	No Hit
TATCAACGCAGAGTACATGGGAAGC	1712	0.3089271278438619	No Hit
CCACTGCTTCCATGTACTCTGCGTT	1673	0.3018896523848019	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1558	0.28113812218500983	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1499	0.270491684952073	No Hit
GTACATGGGTGGTATCAACGCAAAA	1450	0.2616497286060746	No Hit
ACATGGAAGCAGTGGTATCAACGCA	1428	0.25767987065481	No Hit
GGTATCAACGCAGAGTACATGGGAA	1421	0.25641673403395315	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1414	0.2551535974130962	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	1376	0.2482965700427301	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1343	0.24234178311583324	No Hit
GTTGATACCACTGCTTCCATGTACT	1333	0.24053730222889477	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	1276	0.23025176117334564	No Hit
GTACATGGGAGTGGTATCAACGCAA	1275	0.23007131308465179	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1243	0.2242969742464488	No Hit
GATACCACTGCTTCCATGTACTCTG	1114	0.20101917080494283	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	1039	0.1874855641529045	No Hit
CCCCATGTACTCTGCGTTGATACCA	1025	0.18495929091119068	No Hit
GTATCAACGCAGAGTACATGGGGTG	968	0.17467374985564152	No Hit
GCAGTGGTATCAACGCAGAGTACAT	949	0.17124523617045848	No Hit
CTTCCATGTACTCTGCGTTGATACC	934	0.16853851484005083	No Hit
CCATGTACTCTGCGTTGATACCACT	881	0.15897476613927705	No Hit
GTGGTATCAACGCAGAGTACATGGG	776	0.14002771682642337	No Hit
ATCAACGCAGAGTACATGGAAGCAG	753	0.13587741078646495	No Hit
TATCAACGCAGAGTACATGGGGTGG	724	0.13064441621434347	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	723	0.13046396812564962	No Hit
GTCCTACAGTGGACATTTCTAAATT	687	0.12396783693267122	No Hit
TGATACCACTGCTTCCATGTACTCT	679	0.12252425222312045	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	679	0.12252425222312045	No Hit
CACTGCTTCCATGTACTCTGCGTTG	676	0.12198290795703891	No Hit
AAAGTACTCTGCGTTGATACCACTG	670	0.12090021942487586	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	629	0.11350184778842823	No Hit
GTATCAACGCAGAGTACATGGGAGT	623	0.11241915925626515	No Hit
CTGTAGGACGTGGAATATGGCAAGA	582	0.10502078761981752	No Hit
CTTTAGGACGTGAAATATGGCGAGG	555	0.10014868922508373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.8044808869384455E-4	0.0
4	0.0	0.0	0.0	1.8044808869384455E-4	0.0
5	0.0	0.0	0.0	1.8044808869384455E-4	0.0
6	0.0	0.0	0.0	1.8044808869384455E-4	0.0
7	0.0	0.0	0.0	3.608961773876891E-4	0.0
8	0.0	0.0	0.0	3.608961773876891E-4	0.0
9	0.0	0.0	0.0	3.608961773876891E-4	0.0
10	0.0	0.0	0.0	5.413442660815337E-4	0.0
11	0.0	0.0	0.0	5.413442660815337E-4	0.0
12	0.0	0.0	0.0	7.217923547753782E-4	0.0
13	0.0	0.0	0.0	7.217923547753782E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGCC	30	7.7070063E-4	19.002619	15
CGCATCG	25	0.006025628	19.000902	13
AAGTGTC	30	7.711689E-4	19.000902	7
GCGTTAT	35	0.0021736845	16.279139	1
CGTTATT	35	0.0021736845	16.279139	2
TCCAACG	55	1.1232622E-5	15.549002	18
CGCCAGT	45	6.7409396E-4	14.781149	18
CTATGGA	45	6.7594287E-4	14.775811	4
ATCCCGT	65	3.361003E-6	14.616079	10
ACGGTAT	65	3.361003E-6	14.616079	9
ATCGCCA	40	0.0052686064	14.251964	16
TTTGCTT	40	0.0052717645	14.250678	10
TAGAGGC	40	0.005278085	14.248104	5
TAGCCCT	40	0.005278085	14.248104	4
TTTAGGC	40	0.005281247	14.246819	3
CCAACGA	55	1.9527852E-4	13.821335	19
GGTATCT	70	7.2388157E-6	13.572074	11
AGAACAG	130	1.4551915E-11	13.152097	5
ATACCAA	145	0.0	13.106437	19
AAAAAGT	175	0.0	13.023311	1
>>END_MODULE