##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062413_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	554176
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.796862368633793	32.0	32.0	32.0	32.0	32.0
2	31.45749906166994	32.0	32.0	32.0	32.0	32.0
3	31.523297652731262	32.0	32.0	32.0	32.0	32.0
4	31.652121347730684	32.0	32.0	32.0	32.0	32.0
5	31.5898541257651	32.0	32.0	32.0	32.0	32.0
6	35.17898465469454	36.0	36.0	36.0	36.0	36.0
7	35.191690004619474	36.0	36.0	36.0	36.0	36.0
8	35.191388656311354	36.0	36.0	36.0	36.0	36.0
9	35.20923677676406	36.0	36.0	36.0	36.0	36.0
10	35.15337546194711	36.0	36.0	36.0	36.0	36.0
11	35.21139854486661	36.0	36.0	36.0	36.0	36.0
12	35.11353071948262	36.0	36.0	36.0	36.0	36.0
13	35.12278409747084	36.0	36.0	36.0	36.0	36.0
14	35.06411861935558	36.0	36.0	36.0	36.0	36.0
15	35.0601650017323	36.0	36.0	36.0	36.0	36.0
16	35.02023003522347	36.0	36.0	36.0	36.0	36.0
17	34.999765417484696	36.0	36.0	36.0	36.0	36.0
18	34.99840303441506	36.0	36.0	36.0	36.0	36.0
19	34.891875505254646	36.0	36.0	36.0	32.0	36.0
20	34.83108976209724	36.0	36.0	36.0	32.0	36.0
21	34.68687745409401	36.0	36.0	36.0	32.0	36.0
22	34.65013100531239	36.0	36.0	36.0	32.0	36.0
23	34.492960720060054	36.0	36.0	36.0	32.0	36.0
24	34.45583713477307	36.0	36.0	36.0	32.0	36.0
25	34.418412201177965	36.0	36.0	36.0	32.0	36.0
26	34.307339184663356	36.0	36.0	36.0	32.0	36.0
27	34.2210434952073	36.0	36.0	36.0	32.0	36.0
28	34.08322807194826	36.0	36.0	36.0	32.0	36.0
29	33.99773176752512	36.0	36.0	36.0	32.0	36.0
30	33.933402023905764	36.0	36.0	36.0	32.0	36.0
31	33.85764269834854	36.0	36.0	36.0	32.0	36.0
32	33.741008271740384	36.0	36.0	36.0	27.0	36.0
33	33.599730049659314	36.0	36.0	36.0	27.0	36.0
34	33.48687781499019	36.0	36.0	36.0	21.0	36.0
35	33.238415232705854	36.0	36.0	36.0	14.0	36.0
36	33.17957652442546	36.0	36.0	36.0	14.0	36.0
37	32.94529355295069	36.0	36.0	36.0	14.0	36.0
38	32.753192126688994	36.0	36.0	36.0	14.0	36.0
39	32.233656816606995	36.0	36.0	36.0	14.0	36.0
40	31.92686619413327	36.0	36.0	36.0	14.0	36.0
41	31.987471489201987	36.0	36.0	36.0	14.0	36.0
42	31.45291928917889	36.0	32.0	36.0	14.0	36.0
43	31.59452231781961	36.0	32.0	36.0	14.0	36.0
44	31.60276699099203	36.0	32.0	36.0	14.0	36.0
45	30.94484423720984	36.0	32.0	36.0	14.0	36.0
46	31.33754258574893	36.0	32.0	36.0	14.0	36.0
47	30.99028106594295	36.0	32.0	36.0	14.0	36.0
48	31.07839025869038	36.0	32.0	36.0	14.0	36.0
49	31.20023602610001	36.0	32.0	36.0	14.0	36.0
50	30.3897408043654	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	6.0
21	24.0
22	130.0
23	374.0
24	1139.0
25	2928.0
26	6424.0
27	12499.0
28	19762.0
29	25640.0
30	31848.0
31	39086.0
32	53352.0
33	50836.0
34	78656.0
35	231470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.82566323768273	17.02310052337123	14.699512723335136	21.4517235156109
2	10.838338049315702	24.23611913148929	37.553596177166945	27.371946642028067
3	20.51316290459082	30.80294710477452	27.074495056580012	21.609394934054652
4	11.960640662894098	18.78753320244832	32.79896639334796	36.45285974130962
5	10.951394502829427	40.13454209493013	33.65122271624899	15.262840685991454
6	31.681081539519244	31.688660400272116	20.515254261755807	16.11500379845283
7	25.249198810486202	32.72480222889479	23.525558667282596	18.50044029333641
8	30.64207038919044	30.136635292758978	23.139580205566464	16.08171411248412
9	27.93336412980714	16.87712928744659	18.48059100358009	36.70891557916619
10	16.59797609423721	29.044743908072522	32.44564903568541	21.91163096200485
11	33.83185125303152	23.47088289640836	18.92557599029911	23.771689860261
12	21.562283462293568	26.950463390691766	30.42661537128999	21.06063777572468
13	34.03467490472341	21.367940870770298	24.713267987065482	19.884116237440814
14	26.38836759441044	19.504092562651575	25.10105092966855	29.00648891326943
15	27.2749090541633	24.44656571197598	30.915268795472915	17.363256438387804
16	25.290160526619704	24.9707674096316	29.83384339993071	19.905228663817994
17	22.58271740385726	25.117471705739693	31.604941390460795	20.694869499942257
18	26.300850271393927	20.342634830811875	35.128009874119414	18.228505023674792
19	21.35855757015822	33.482684201408944	27.079122877930477	18.079635350502368
20	21.774309966508834	20.995676463794897	37.98594670285252	19.244066866843745
21	22.722745120683683	28.468031816607	23.652594121723062	25.15662894098626
22	20.29842504908188	29.296829887977825	26.719489837163646	23.68525522577665
23	19.17910555491396	27.46149237787273	26.272880817646378	27.086521249566925
24	18.618814239519573	32.326011952881395	27.018131423951957	22.037042383647073
25	25.73604775378219	24.09324835431343	24.32783086961543	25.84287302228895
26	18.00980194017785	25.033563344497058	29.612253147014666	27.34438156831043
27	25.70880009238942	28.27946356392193	28.437355641529045	17.574380702159605
28	22.36239028756207	22.377367478923663	28.961196443007275	26.299045790506987
29	18.99829657004273	28.46334016630096	32.83198839357894	19.706374870077376
30	25.77448319667398	24.26449359048389	30.61121376602379	19.349809446818337
31	18.530394676059593	26.473899988451322	29.089675482157297	25.90602985333179
32	19.136519805982218	28.71686973091581	29.633365573391846	22.51324488971013
33	18.95787619817531	27.340050814181776	29.363415232705854	24.33865775493706
34	17.420999826769833	30.421743272895252	32.61779651229934	19.53946038803557
35	23.65981204527082	27.410064672594984	29.385429899526507	19.54469338260769
36	21.241266312507218	29.3298518882088	29.317400970088926	20.11148082919506
37	23.997430419217	28.635126746737498	29.278965527197137	18.088477306848365
38	21.581952303961195	29.018218039034533	24.839762097239866	24.560067559764406
39	20.084954960157063	27.83123051160642	30.43563777572468	21.648176752511837
40	23.465649901836237	28.212517323016517	25.650334911652617	22.67149786349463
41	18.19421988682296	28.25600531239173	29.80154319205451	23.748231608730798
42	25.60053123917311	28.613833872271627	28.23200571659545	17.553629171959813
43	23.151850675597643	24.56818772375563	25.83024165608038	26.449719944566347
44	21.629951495553758	26.427344381568314	32.64811179119991	19.29459233167802
45	23.95989721676868	26.797082515301994	27.75959262039496	21.483427647534356
46	20.20751567645599	26.184508503631527	32.25731943880543	21.35065638110705
47	23.924529391384684	24.39369442198868	32.17840541632983	19.5033707702968
48	19.256337336874928	35.63921930938908	24.395679350964315	20.70876400277168
49	18.540499769026447	26.562860896177387	35.05312391731147	19.843515417484696
50	18.021711514031644	35.14695692343226	22.52172594987874	24.30960561265735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	37.5
2	43.0
3	199.5
4	356.0
5	396.0
6	436.0
7	425.5
8	415.0
9	539.0
10	663.0
11	1014.0
12	1365.0
13	2322.5
14	3280.0
15	4500.0
16	5720.0
17	6789.0
18	7858.0
19	8328.5
20	8799.0
21	9362.0
22	9925.0
23	9932.0
24	9939.0
25	8170.5
26	6402.0
27	7038.0
28	7674.0
29	8749.0
30	9824.0
31	11134.5
32	12445.0
33	14608.5
34	16772.0
35	18430.5
36	20089.0
37	20513.5
38	20938.0
39	20659.5
40	20381.0
41	22319.5
42	24258.0
43	30139.0
44	36020.0
45	48653.5
46	61287.0
47	71677.5
48	82068.0
49	71474.5
50	60881.0
51	44750.5
52	28620.0
53	22464.5
54	16309.0
55	15347.5
56	14386.0
57	14056.0
58	13726.0
59	12730.0
60	11734.0
61	10385.5
62	9037.0
63	8215.5
64	7394.0
65	6842.0
66	6290.0
67	6000.5
68	5711.0
69	5293.0
70	4875.0
71	3826.0
72	2777.0
73	2414.0
74	2051.0
75	1661.5
76	1272.0
77	1081.5
78	891.0
79	715.5
80	540.0
81	412.5
82	285.0
83	242.0
84	199.0
85	161.5
86	124.0
87	89.5
88	55.0
89	35.0
90	15.0
91	9.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	3.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013714054740732187
2	0.005774338838203026
3	9.022404434692227E-4
4	0.0
5	0.0
6	5.413442660815337E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.8044808869384455E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	554176.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.832716777506874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.88423382544495	33.41774528607973
2	10.621242894256588	8.886308916410862
3	3.444388260529853	4.3226435572354465
4	1.5588341685903173	2.6084107311095655
5	0.9145702054238931	1.9129478188322002
6	0.5883844953881655	1.4768233171109657
7	0.41409113519299173	1.212579002502331
8	0.3217150555665227	1.0766571842059383
9	0.23930816371465977	0.9009819571698549
>10	1.673307113501276	13.720519485219263
>50	0.18734979298096943	5.47414406794512
>100	0.1304058883844576	10.296387840712434
>500	0.011736529954601182	3.2562283358995034
>1k	0.00956309848152689	7.664783636929689
>5k	4.3468629461485865E-4	1.798772027627067
>10k+	4.3468629461485865E-4	1.9740668350100525
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	10856	1.9589444508603764	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	9892	1.7849924933595103	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	4160	0.7506640489663934	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	3988	0.7196269777110521	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	2639	0.47620250606305575	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	2461	0.4440827462755514	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	2388	0.43091003580090076	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2348	0.42369211225314707	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2330	0.4204440466566578	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	2222	0.40095565307772263	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2196	0.3962640027716826	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1835	0.33112224275320473	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	1523	0.27482243908072523	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	1463	0.2639955537590946	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	1453	0.26219107287215615	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1437	0.2593039034530546	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	1381	0.24919881048619932	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	1305	0.23548475574546715	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1232	0.22231204527081652	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1208	0.21798129114216422	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	1190	0.214733225545675	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1146	0.20679350964314586	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	1133	0.20444768449012587	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1113	0.20083872271624897	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	948	0.17106478808176462	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	886	0.15987700658274628	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	844	0.1522981868576048	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	824	0.14868922508372792	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	813	0.1467042961080956	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	803	0.1448998152211572	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	773	0.13948637256034183	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA	703	0.12685500635177271	No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	689	0.1243287331100589	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	686	0.12378738884397736	No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	686	0.12378738884397736	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	676	0.12198290795703891	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	656	0.11837394618316203	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG	644	0.1162085691188359	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	634	0.11440408823189745	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTA	627	0.11314095161104054	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTATGCGTTGATA	615	0.1109755745467144	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTA	588	0.1061034761519806	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	578	0.10429899526504215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.8044808869384455E-4	0.0
8	5.413442660815337E-4	0.0	0.0	1.8044808869384455E-4	0.0
9	5.413442660815337E-4	0.0	0.0	1.8044808869384455E-4	0.0
10	5.413442660815337E-4	0.0	0.0	3.608961773876891E-4	0.0
11	5.413442660815337E-4	0.0	0.0	3.608961773876891E-4	0.0
12	5.413442660815337E-4	0.0	0.0	5.413442660815337E-4	0.0
13	5.413442660815337E-4	0.0	0.0	7.217923547753782E-4	0.0
14	5.413442660815337E-4	0.0	0.0	9.022404434692227E-4	0.0
15	5.413442660815337E-4	0.0	0.0	0.0010826885321630674	0.0
16	5.413442660815337E-4	0.0	0.0	0.0018044808869384454	0.0
17	5.413442660815337E-4	0.0	0.0	0.0021653770643261347	0.0
18	5.413442660815337E-4	0.0	0.0	0.0023458251530199792	0.0
19	5.413442660815337E-4	0.0	0.0	0.0034285136851830464	0.0
20	5.413442660815337E-4	0.0	0.0	0.004150306039958425	0.0
21	5.413442660815337E-4	0.0	0.0	0.005774338838203026	0.0
22	5.413442660815337E-4	0.0	0.0	0.00956374870077376	0.0
23	5.413442660815337E-4	0.0	0.0	0.018766601224159834	0.0
24	5.413442660815337E-4	0.0	0.0	0.03175886361011664	0.0
25	5.413442660815337E-4	0.0	0.0	0.04150306039958425	0.0
26	5.413442660815337E-4	0.0	0.0	0.05485621896292874	0.0
27	5.413442660815337E-4	0.0	0.0	0.07344237209839473	0.0
28	5.413442660815337E-4	0.0	0.0	0.10484033953112369	0.0
29	5.413442660815337E-4	0.0	0.0	0.1438171266889941	0.0
30	5.413442660815337E-4	0.0	0.0	0.22628190322208108	0.0
31	5.413442660815337E-4	0.0	0.0	0.3527760133964661	0.0
32	5.413442660815337E-4	0.0	0.0	0.518968703083497	0.0
33	5.413442660815337E-4	0.0	0.0	0.6483499826769835	0.0
34	5.413442660815337E-4	0.0	0.0	0.8410685414020095	0.0
35	5.413442660815337E-4	0.0	0.0	1.0197121492089156	0.0
36	5.413442660815337E-4	0.0	0.0	1.3003089271278438	0.0
37	5.413442660815337E-4	0.0	0.0	1.7295949301305	0.0
38	5.413442660815337E-4	0.0	0.0	2.098791719598106	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGG	25	0.0023518153	35.19964	44
TCGTATA	115	0.0	34.43443	44
GCGATTA	35	3.2158243E-4	31.428247	42
TATTAGT	30	0.00574265	29.333035	4
TTAACGG	90	1.6370905E-11	29.333033	35
CGATTAT	55	4.9550817E-6	27.999714	43
TAACGGC	95	3.092282E-11	27.789188	36
CTCGTAT	65	5.921993E-7	27.076645	43
GCCCAGT	100	6.002665E-11	26.399729	19
GTGACTA	100	6.002665E-11	26.399729	24
AGTTTAA	110	7.2759576E-12	25.999733	32
GACGTGG	180	0.0	24.444193	7
TGCTGTA	45	0.0013973432	24.444193	12
TGTAGGA	370	0.0	24.380327	2
AGTACTC	445	0.0	24.22447	5
ATACACT	200	0.0	24.19975	4
GGCCGCG	110	2.0008883E-10	23.999754	40
TGACTAA	110	2.0008883E-10	23.999754	25
TATAGGA	65	1.8099725E-5	23.694202	2
AAGTACT	455	0.0	23.692066	4
>>END_MODULE