FastQCFastQC Report
Thu 2 Feb 2017
SRR4062412_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062412_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951611
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32240.16519685531594153No Hit
TATCAACGCAGAGTACTTTTTTTTT19770.10130092523561304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG3650.012.4910255
GCGCAAG3900.012.41332151
CGCAAGA3800.011.9939592
CAAGACG3650.011.9696454
CGAACGA1052.712568E-711.76473216
ACAATAC2100.011.7580953
GTATCAA22400.011.3995231
ACTAGAC752.0805823E-411.3963073
CCGACCA2550.011.1774379
GACGGAC3950.011.0645317
GTTTTCG1553.1286618E-1011.03349515
ACGGTAT3100.011.0332129
CGACCAT2500.011.02123510
CGGTGTT951.3596586E-511.00066914
GCTCTAA951.3724735E-510.9913621
GTCTTAC951.3724735E-510.9913621
CAATACA2600.010.9588314
GCAAGAC5350.010.8282673
ACGAACG1158.8062734E-710.74033415
CGGTTCT3200.010.68924612
Produced by FastQC (version 0.11.5)