##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062412_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1951611
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266525450000024	32.0	32.0	32.0	32.0	32.0
2	31.03736502817416	32.0	32.0	32.0	32.0	32.0
3	31.022355889570207	32.0	32.0	32.0	32.0	32.0
4	31.0415923050239	32.0	32.0	32.0	32.0	32.0
5	30.938613278978238	32.0	32.0	32.0	32.0	32.0
6	34.51326007078255	36.0	36.0	36.0	32.0	36.0
7	34.457259156665955	36.0	36.0	36.0	32.0	36.0
8	34.46501531299014	36.0	36.0	36.0	32.0	36.0
9	34.56716374318448	36.0	36.0	36.0	32.0	36.0
10	34.34516048536312	36.0	36.0	36.0	32.0	36.0
11	34.53696202778115	36.0	36.0	36.0	32.0	36.0
12	34.388743453485354	36.0	36.0	36.0	32.0	36.0
13	34.47774325928682	36.0	36.0	36.0	32.0	36.0
14	34.41437048674147	36.0	36.0	36.0	32.0	36.0
15	34.358983424463176	36.0	36.0	36.0	32.0	36.0
16	34.35767886120748	36.0	36.0	36.0	32.0	36.0
17	34.29880288643587	36.0	36.0	36.0	32.0	36.0
18	34.29258699607657	36.0	36.0	36.0	32.0	36.0
19	34.29021972104072	36.0	36.0	36.0	32.0	36.0
20	34.27854628816911	36.0	36.0	36.0	32.0	36.0
21	34.254649107839626	36.0	36.0	36.0	32.0	36.0
22	34.21154984266844	36.0	36.0	36.0	32.0	36.0
23	34.184295435924476	36.0	36.0	36.0	32.0	36.0
24	34.15609565635775	36.0	36.0	36.0	32.0	36.0
25	33.672047861997086	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	81.0
5	158.0
6	356.0
7	73.0
8	287.0
9	328.0
10	258.0
11	83.0
12	138.0
13	125.0
14	285.0
15	393.0
16	654.0
17	903.0
18	1235.0
19	1613.0
20	2251.0
21	2961.0
22	4161.0
23	5994.0
24	8367.0
25	11667.0
26	16948.0
27	22507.0
28	31907.0
29	44255.0
30	59693.0
31	86089.0
32	130477.0
33	198942.0
34	474269.0
35	844146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.183249292829906	17.454085188373714	10.994906325585209	26.367759193211164
2	17.13496157921814	19.864948235964476	36.44009780953377	26.55999237528362
3	19.058221283922276	23.32935352955645	28.439011642207102	29.173413544314176
4	12.965163742249514	15.125076294156445	35.492682166871916	36.41707779672213
5	14.968747725406454	36.005316526030576	33.537786188719245	15.488149559843723
6	34.56216284383329	34.97647756615476	16.62518563934524	13.836173950666714
7	30.300441728046266	30.251218405420722	20.83853981064403	18.609800055888982
8	28.234763164912714	32.59195437667959	19.588487496666463	19.58479496174124
9	27.40691807190109	14.195167135619528	18.59828058596711	39.79963420651227
10	15.97487490291317	26.51032224106673	31.603879574367227	25.91092328165287
11	37.60921502669487	21.06691236787874	22.027024739246	19.296847866180393
12	24.594764883591107	23.566900223294915	28.566561586707383	23.271773306406594
13	29.322978469948886	19.293782310852137	25.247264431218703	26.13597478798027
14	23.43319050433412	19.57803881008668	24.792272048200683	32.19649863737851
15	25.264211397845273	26.87138411787096	22.099163531491453	25.76524095279232
16	25.91434419291793	25.52294773344003	23.551089292767628	25.01161878087441
17	24.17624023767663	25.584610735772518	25.087330565364407	25.151818461186444
18	25.077998857915095	24.652256695126436	25.878587036771954	24.391157410186516
19	25.6203579615769	24.892972731134776	25.162285355426988	24.324383951861332
20	25.56919269545446	24.438912977901268	24.799307631850574	25.192586694793697
21	26.6345658046294	24.14716800106718	24.393952997975955	24.82431319632747
22	25.865749659509262	24.26700837191544	24.647167435229782	25.22007453334551
23	24.721969941864344	24.097973718373023	25.287163462278528	25.89289287748411
24	24.932387814567907	24.927205624677075	24.855527215989056	25.284879344765965
25	24.891908046802648	24.451382350753065	25.141159599494106	25.515550002950178
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	150.0
1	150.0
2	464.0
3	778.0
4	778.0
5	778.0
6	1960.0
7	3142.0
8	3142.0
9	3142.0
10	3538.5
11	3935.0
12	3935.0
13	3935.0
14	4902.5
15	5870.0
16	5870.0
17	5870.0
18	9296.5
19	12723.0
20	12723.0
21	12723.0
22	20279.0
23	27835.0
24	27835.0
25	27835.0
26	41290.0
27	54745.0
28	54745.0
29	54745.0
30	67999.5
31	81254.0
32	81254.0
33	81254.0
34	100542.0
35	119830.0
36	119830.0
37	119830.0
38	136942.5
39	154055.0
40	154055.0
41	154055.0
42	175757.5
43	197460.0
44	197460.0
45	197460.0
46	222697.0
47	247934.0
48	247934.0
49	247934.0
50	254357.5
51	260781.0
52	260781.0
53	260781.0
54	245453.5
55	230126.0
56	230126.0
57	230126.0
58	211994.5
59	193863.0
60	193863.0
61	193863.0
62	171512.5
63	149162.0
64	149162.0
65	149162.0
66	123646.0
67	98130.0
68	98130.0
69	98130.0
70	74548.5
71	50967.0
72	50967.0
73	50967.0
74	39743.0
75	28519.0
76	28519.0
77	28519.0
78	23203.0
79	17887.0
80	17887.0
81	17887.0
82	12548.0
83	7209.0
84	7209.0
85	7209.0
86	5178.5
87	3148.0
88	3148.0
89	3148.0
90	2156.0
91	1164.0
92	1164.0
93	1164.0
94	682.0
95	200.0
96	200.0
97	200.0
98	472.0
99	744.0
100	744.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008761992015826925
2	0.0032281023216204456
3	0.004662814464562866
4	0.014449600868205806
5	0.036533919925640916
6	0.04714054183953667
7	0.06743147071829377
8	0.08869595426547607
9	0.09617695329653296
10	0.1191323475836117
11	0.12092573776228972
12	0.13419682508450712
13	0.12205301158888734
14	0.12435879896147337
15	0.11400837564453162
16	0.11175382799133639
17	0.12338524429304815
18	0.13035384613019704
19	0.13194227743131187
20	0.12087449804289892
21	0.13045632556897865
22	0.15131089136103457
23	0.13942327646236877
24	0.1346579825590243
25	0.13199351715070268
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1951611.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.481519696999605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.18430862806777	43.685629899103695
2	11.87054040480585	12.934501617569188
3	3.3421165822299788	5.462507712132947
4	1.4404299817547135	3.1390725769247276
5	0.7812629836073895	2.1282197314971336
6	0.48145133800685846	1.5738120332860484
7	0.33507267069840097	1.2778687816006835
8	0.2321077778318617	1.0116467575818704
9	0.18773459743275117	0.9205259551056653
>10	0.9739922705354022	9.488899295279987
>50	0.07733069471098555	2.9183085395130623
>100	0.07981750125949534	9.179014299616355
>500	0.01072884947405519	3.841297044037591
>1k	0.003105719584594923	2.438695756751016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3224	0.16519685531594153	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1977	0.10130092523561304	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.123971939080073E-5
2	0.0	0.0	0.0	0.0	5.123971939080073E-5
3	0.0	0.0	0.0	0.0	5.123971939080073E-5
4	0.0	0.0	0.0	0.0	5.123971939080073E-5
5	0.0	0.0	0.0	0.0	5.123971939080073E-5
6	0.0	0.0	0.0	0.0	5.123971939080073E-5
7	0.0	0.0	0.0	1.0247943878160146E-4	5.123971939080073E-5
8	0.0	0.0	0.0	1.0247943878160146E-4	5.123971939080073E-5
9	0.0	0.0	0.0	1.0247943878160146E-4	1.5371915817240217E-4
10	0.0	0.0	0.0	1.0247943878160146E-4	1.5371915817240217E-4
11	0.0	0.0	0.0	1.0247943878160146E-4	1.5371915817240217E-4
12	0.0	0.0	0.0	2.5619859695400366E-4	2.5619859695400366E-4
13	0.0	0.0	0.0	2.5619859695400366E-4	2.5619859695400366E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	365	0.0	12.491025	5
GCGCAAG	390	0.0	12.4133215	1
CGCAAGA	380	0.0	11.993959	2
CAAGACG	365	0.0	11.969645	4
CGAACGA	105	2.712568E-7	11.764732	16
ACAATAC	210	0.0	11.758095	3
GTATCAA	2240	0.0	11.399523	1
ACTAGAC	75	2.0805823E-4	11.396307	3
CCGACCA	255	0.0	11.177437	9
GACGGAC	395	0.0	11.064531	7
GTTTTCG	155	3.1286618E-10	11.033495	15
ACGGTAT	310	0.0	11.033212	9
CGACCAT	250	0.0	11.021235	10
CGGTGTT	95	1.3596586E-5	11.000669	14
GCTCTAA	95	1.3724735E-5	10.991362	1
GTCTTAC	95	1.3724735E-5	10.991362	1
CAATACA	260	0.0	10.958831	4
GCAAGAC	535	0.0	10.828267	3
ACGAACG	115	8.8062734E-7	10.740334	15
CGGTTCT	320	0.0	10.689246	12
>>END_MODULE