FastQCFastQC Report
Thu 2 Feb 2017
SRR4062412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951611
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27000.020.4538881
TATACCG654.9337547E-420.3076325
TAACGGC2800.019.64280136
TTAACGG2850.018.52626235
GGCGTTA3550.017.35206242
ACTATAC1854.2382453E-1016.64863
GTTATAT2950.016.4084131
ATACCGT5350.016.0373366
GTATAGG2750.016.0015931
TACGCTA5250.015.9237629
GGTATCA14800.015.7583261
ACGCTAT5350.015.62612310
AACGCAG35700.015.4061176
CGTACAC1004.9457385E-415.3999553
ATACGCT5450.015.3394058
CAACGGA2900.015.17237114
ACCGTCG4950.015.1110668
GTATTAG6700.015.1059811
GCGAAAG7600.015.052587518
AACGGAC2800.014.92852915
Produced by FastQC (version 0.11.5)