##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062412_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1951611
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25854640089649	32.0	32.0	32.0	32.0	32.0
2	31.297327182517417	32.0	32.0	32.0	32.0	32.0
3	31.413171989704917	32.0	32.0	32.0	32.0	32.0
4	31.549900056927328	32.0	32.0	32.0	32.0	32.0
5	31.442288447851546	32.0	32.0	32.0	32.0	32.0
6	35.04629149968923	36.0	36.0	36.0	36.0	36.0
7	35.06614791574756	36.0	36.0	36.0	36.0	36.0
8	34.99015018874151	36.0	36.0	36.0	36.0	36.0
9	35.15779988942469	36.0	36.0	36.0	36.0	36.0
10	35.001429588171	36.0	36.0	36.0	36.0	36.0
11	35.16309295243776	36.0	36.0	36.0	36.0	36.0
12	35.05913012377979	36.0	36.0	36.0	36.0	36.0
13	35.11841089233459	36.0	36.0	36.0	36.0	36.0
14	35.07476028778276	36.0	36.0	36.0	36.0	36.0
15	35.02001525918843	36.0	36.0	36.0	36.0	36.0
16	35.039930088526866	36.0	36.0	36.0	36.0	36.0
17	35.011456176461394	36.0	36.0	36.0	36.0	36.0
18	35.025691082905354	36.0	36.0	36.0	36.0	36.0
19	35.016725669203545	36.0	36.0	36.0	36.0	36.0
20	35.00765675126857	36.0	36.0	36.0	36.0	36.0
21	35.00689789102439	36.0	36.0	36.0	36.0	36.0
22	34.98541307668383	36.0	36.0	36.0	36.0	36.0
23	34.950405075601644	36.0	36.0	36.0	32.0	36.0
24	34.92543032397337	36.0	36.0	36.0	32.0	36.0
25	34.89835730583605	36.0	36.0	36.0	32.0	36.0
26	34.84706019795953	36.0	36.0	36.0	32.0	36.0
27	34.8322426959061	36.0	36.0	36.0	32.0	36.0
28	34.82004866748548	36.0	36.0	36.0	32.0	36.0
29	34.797198827020345	36.0	36.0	36.0	32.0	36.0
30	34.78757447052717	36.0	36.0	36.0	32.0	36.0
31	34.782209671906955	36.0	36.0	36.0	32.0	36.0
32	34.754131842872376	36.0	36.0	36.0	32.0	36.0
33	34.7310585972307	36.0	36.0	36.0	32.0	36.0
34	34.726914841123566	36.0	36.0	36.0	32.0	36.0
35	34.70882414579545	36.0	36.0	36.0	32.0	36.0
36	34.677478247458126	36.0	36.0	36.0	32.0	36.0
37	34.67634687445398	36.0	36.0	36.0	32.0	36.0
38	34.64020135160132	36.0	36.0	36.0	32.0	36.0
39	34.63622258739062	36.0	36.0	36.0	32.0	36.0
40	34.632369872889626	36.0	36.0	36.0	32.0	36.0
41	34.60079647019821	36.0	36.0	36.0	32.0	36.0
42	34.585266223648055	36.0	36.0	36.0	32.0	36.0
43	34.580086400414835	36.0	36.0	36.0	32.0	36.0
44	34.55104219027255	36.0	36.0	36.0	32.0	36.0
45	34.518520852772404	36.0	36.0	36.0	32.0	36.0
46	34.52636667860552	36.0	36.0	36.0	32.0	36.0
47	34.50490748412465	36.0	36.0	36.0	32.0	36.0
48	34.46593865273356	36.0	36.0	36.0	32.0	36.0
49	34.46429590732989	36.0	36.0	36.0	32.0	36.0
50	33.89832092563528	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	13.0
21	42.0
22	147.0
23	408.0
24	1135.0
25	2603.0
26	5562.0
27	10565.0
28	18162.0
29	29141.0
30	43253.0
31	64741.0
32	99144.0
33	170085.0
34	377548.0
35	1129059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.498186659533225	18.06975710923243	11.547368491178766	26.88468774005558
2	16.554285506391608	20.296760050893063	36.139044848102245	27.009909594613084
3	18.492150803366055	23.67558737672301	28.801583930321822	29.030677889589114
4	12.761496306885872	15.665926250445148	35.75503239641218	35.8175450462568
5	14.837178105677824	36.13870796997967	33.37519618407562	15.648917740266889
6	34.80890363684902	35.25381903221294	16.307432797689287	13.629844533248752
7	30.379363510453672	30.292307227208703	20.79461532036866	18.53371394196897
8	28.22217132410096	32.779431966718775	19.37922055163657	19.61917615754369
9	27.54698554168838	13.982243387642313	18.398236123899693	40.072534946769615
10	15.950002331407232	26.52711016693388	31.5956919693525	25.927195532306385
11	37.801488103930545	20.86522365368918	22.114191813839952	19.21909642854032
12	24.550076833959224	23.509551852290237	28.52535674373633	23.415014570014208
13	29.512284978922544	19.12814592662165	25.19938655807945	26.160182536376357
14	23.499355148131468	19.424413984139257	24.72101253784694	32.35521832988234
15	25.36417349564027	26.95890728224016	21.876593235024806	25.800325987094762
16	26.09951470861765	25.459991770901063	23.3810938757775	25.059399644703785
17	24.41393289953787	25.586348918918784	24.89158956369891	25.10812861784444
18	25.238226265377676	24.672949681058366	25.671765531143247	24.417058522420707
19	25.8096003763045	24.856439116196825	24.89594493984713	24.438015567651544
20	25.766610251735617	24.337944395681312	24.614536400952854	25.280908951630217
21	26.855249329912567	24.13754585314389	24.093633413625973	24.913571403317565
22	25.936623640674295	24.268565815626168	24.489972643113816	25.30483790058572
23	24.875910209565326	23.994535796324165	25.050637652687957	26.07891634142255
24	25.09649720154273	24.852083740048606	24.727724941087132	25.323694117321537
25	25.191239442696318	24.322111322389553	24.918541656098476	25.568107578815656
26	24.911521814541935	24.871811032014065	25.30596517441232	24.910701979031682
27	25.547509211620557	24.688321596875607	24.46132963997436	25.302839551529484
28	24.90742263699067	24.527121439672147	25.12570384159548	25.4397520817417
29	24.961583020386747	24.681711673074194	24.96368384888177	25.393021457657287
30	24.849982911553585	24.815344861245404	25.209788221115787	25.124884006085228
31	25.277373410992254	24.599625642610132	24.426486630788617	25.696514315609004
32	25.024761594395606	24.642769486337183	24.50170653885431	25.830762380412896
33	24.822262223363158	24.336304724660806	24.965169800744103	25.87626325123193
34	25.36371233816575	24.403941154256664	25.259234550327907	24.973111957249678
35	25.829122709392394	24.336663402696544	24.98540948990347	24.848804398007594
36	24.903835856633314	24.806070472035664	24.71537616871395	25.574717502617066
37	25.674942393745475	24.514618948140793	24.586508274446086	25.223930383667646
38	24.937654071431243	24.399483298669665	25.126677396263908	25.536185233635187
39	25.392150382427648	24.292546004301062	24.555918161969778	25.759385451301515
40	25.596853061393894	24.377860137086746	25.18898489504312	24.836301906476237
41	24.658038922715644	24.601931429982717	25.534853000931022	25.205176646370614
42	25.59982496511856	24.844500261578766	24.94764581671245	24.60802895659022
43	24.82605396259808	23.96671262869496	25.303813106197904	25.903420302509055
44	24.603519861283832	24.45733294186188	25.143176585907746	25.795970610946544
45	24.703334834657113	24.58297273380812	25.27532382221662	25.438368609318147
46	24.628524082973673	24.262108098607968	25.373704849388222	25.735662969030138
47	24.74289189802681	24.174131012788923	25.604692738460688	25.47828435072358
48	25.247141976551678	24.837121741986493	24.935297044339265	24.98043923712256
49	24.364537810045135	24.77983573570758	25.19129068241571	25.66433577183158
50	24.18668474403967	24.957535082554873	25.20753367346259	25.648246499942868
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	9.0
2	11.0
3	54.5
4	98.0
5	121.0
6	144.0
7	157.5
8	171.0
9	250.5
10	330.0
11	458.0
12	586.0
13	1052.5
14	1519.0
15	1984.5
16	2450.0
17	2989.0
18	3528.0
19	3933.5
20	4339.0
21	5415.0
22	6491.0
23	7367.5
24	8244.0
25	10419.0
26	12594.0
27	15989.0
28	19384.0
29	22137.0
30	24890.0
31	27019.5
32	29149.0
33	33705.0
34	38261.0
35	44965.0
36	51669.0
37	61755.5
38	71842.0
39	77606.5
40	83371.0
41	91367.5
42	99364.0
43	102059.0
44	104754.0
45	114083.5
46	123413.0
47	131634.5
48	139856.0
49	144617.5
50	149379.0
51	146049.5
52	142720.0
53	140564.0
54	138408.0
55	139380.5
56	140353.0
57	137071.5
58	133790.0
59	123072.5
60	112355.0
61	99289.0
62	86223.0
63	75535.5
64	64848.0
65	55693.0
66	46538.0
67	40337.5
68	34137.0
69	30526.5
70	26916.0
71	21380.5
72	15845.0
73	14325.0
74	12805.0
75	9609.0
76	6413.0
77	5774.5
78	5136.0
79	4463.0
80	3790.0
81	3046.5
82	2303.0
83	1950.0
84	1597.0
85	1273.0
86	949.0
87	687.0
88	425.0
89	286.5
90	148.0
91	92.0
92	36.0
93	27.0
94	18.0
95	11.5
96	5.0
97	5.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012297532653792175
2	0.0034843009185744496
3	5.123971939080073E-4
4	3.0743831634480435E-4
5	0.0
6	2.0495887756320292E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.0495887756320292E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1951611.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.033316320537445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31901021750613	47.192118428255505
2	11.421196568919287	13.256198264862599
3	3.0946850861810713	5.387845155563873
4	1.3153015620256412	3.053252464237241
5	0.7139463748034317	2.071633790243427
6	0.44661918216624913	1.5551275364084203
7	0.3092630976273483	1.2563294219614012
8	0.22024201119844378	1.022509944236051
9	0.16778281005865636	0.8763293600354389
>10	0.8493924495595868	8.737831857426768
>50	0.06624675260359766	2.657145209087589
>100	0.0665655461064301	8.281229659227765
>500	0.007621430427419425	2.981389569355923
>1k	0.002126910816954258	1.671059339098027
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.123971939080073E-5
2	0.0	0.0	0.0	0.0	5.123971939080073E-5
3	0.0	0.0	0.0	0.0	5.123971939080073E-5
4	0.0	0.0	0.0	0.0	5.123971939080073E-5
5	5.123971939080073E-5	0.0	0.0	0.0	5.123971939080073E-5
6	5.123971939080073E-5	0.0	0.0	0.0	5.123971939080073E-5
7	5.123971939080073E-5	0.0	0.0	1.0247943878160146E-4	5.123971939080073E-5
8	5.123971939080073E-5	0.0	0.0	1.0247943878160146E-4	5.123971939080073E-5
9	5.123971939080073E-5	0.0	0.0	1.0247943878160146E-4	5.123971939080073E-5
10	5.123971939080073E-5	0.0	0.0	1.0247943878160146E-4	5.123971939080073E-5
11	5.123971939080073E-5	0.0	0.0	1.0247943878160146E-4	5.123971939080073E-5
12	5.123971939080073E-5	0.0	0.0	1.5371915817240217E-4	1.0247943878160146E-4
13	5.123971939080073E-5	0.0	0.0	1.5371915817240217E-4	1.0247943878160146E-4
14	5.123971939080073E-5	0.0	0.0	1.5371915817240217E-4	1.0247943878160146E-4
15	5.123971939080073E-5	0.0	0.0	2.5619859695400366E-4	1.0247943878160146E-4
16	5.123971939080073E-5	0.0	0.0	2.5619859695400366E-4	1.0247943878160146E-4
17	5.123971939080073E-5	0.0	0.0	3.0743831634480435E-4	1.0247943878160146E-4
18	5.123971939080073E-5	0.0	0.0	3.0743831634480435E-4	1.0247943878160146E-4
19	5.123971939080073E-5	0.0	0.0	3.0743831634480435E-4	1.5371915817240217E-4
20	5.123971939080073E-5	0.0	0.0	7.173560714712102E-4	1.5371915817240217E-4
21	5.123971939080073E-5	0.0	0.0	8.710752296436124E-4	1.5371915817240217E-4
22	5.123971939080073E-5	0.0	0.0	0.0011785135459884168	1.5371915817240217E-4
23	5.123971939080073E-5	0.0	0.0	0.0015884313011148225	1.5371915817240217E-4
24	5.123971939080073E-5	0.0	0.0	0.002203307933804431	1.5371915817240217E-4
25	5.123971939080073E-5	0.0	0.0	0.002613225688930837	1.5371915817240217E-4
26	5.123971939080073E-5	0.0	0.0	0.0034330611991836486	1.5371915817240217E-4
27	5.123971939080073E-5	0.0	0.0	0.005072732219689272	1.5371915817240217E-4
28	5.123971939080073E-5	0.0	0.0	0.012143813495619772	1.5371915817240217E-4
29	5.123971939080073E-5	0.0	0.0	0.024338866710630347	1.5371915817240217E-4
30	5.123971939080073E-5	0.0	0.0	0.049087651176387095	1.5371915817240217E-4
31	5.123971939080073E-5	0.0	0.0	0.10709101352677353	1.5371915817240217E-4
32	5.123971939080073E-5	0.0	0.0	0.17482992256141208	1.5371915817240217E-4
33	5.123971939080073E-5	0.0	0.0	0.2465142899891423	1.5371915817240217E-4
34	5.123971939080073E-5	0.0	0.0	0.324757341498895	1.5371915817240217E-4
35	5.123971939080073E-5	0.0	0.0	0.410378912600923	1.5371915817240217E-4
36	5.123971939080073E-5	0.0	0.0	0.5365311017410744	1.5371915817240217E-4
37	5.123971939080073E-5	0.0	0.0	0.7208403723897846	1.5371915817240217E-4
38	5.123971939080073E-5	0.0	0.0	0.9618207726847204	1.5371915817240217E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2700	0.0	20.453888	1
TATACCG	65	4.9337547E-4	20.307632	5
TAACGGC	280	0.0	19.642801	36
TTAACGG	285	0.0	18.526262	35
GGCGTTA	355	0.0	17.352062	42
ACTATAC	185	4.2382453E-10	16.6486	3
GTTATAT	295	0.0	16.408413	1
ATACCGT	535	0.0	16.037336	6
GTATAGG	275	0.0	16.001593	1
TACGCTA	525	0.0	15.923762	9
GGTATCA	1480	0.0	15.758326	1
ACGCTAT	535	0.0	15.626123	10
AACGCAG	3570	0.0	15.406117	6
CGTACAC	100	4.9457385E-4	15.399955	3
ATACGCT	545	0.0	15.339405	8
CAACGGA	290	0.0	15.172371	14
ACCGTCG	495	0.0	15.111066	8
GTATTAG	670	0.0	15.105981	1
GCGAAAG	760	0.0	15.0525875	18
AACGGAC	280	0.0	14.928529	15
>>END_MODULE