FastQCFastQC Report
Thu 2 Feb 2017
SRR4062411_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062411_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872168
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55660.29730237884634286No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43670.23325898103161682No Hit
GTACATGGAAGCAGTGGTATCAACG39920.21322872733643564No Hit
TATCAACGCAGAGTACTTTTTTTTT38170.20388127561201774No Hit
TCCATGTACTCTGCGTTGATACCAC34180.1825690856803449No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30990.16553001653697744No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22990.12279880865392423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA456.759486E-414.77885911
GCGTTAT1351.8189894E-1213.3638431
AATCGTA802.000179E-613.062407513
CGGACAT2150.012.8131795
TCGAACT1051.9877916E-812.66962719
GTCGCGT1352.7284841E-1112.6675948
CGCATCG2650.012.54708213
CGGTTCT4100.012.28138612
CGTTATT1405.2750693E-1112.2096292
CGCCAGT2500.012.16251618
TCGCGTA1251.4097168E-912.1605649
CTAGACA1103.822788E-812.0892084
GGTTCTA4100.012.04869613
GAACTAG1501.4551915E-1112.0345366
AACCGCG1201.0002623E-811.8761877
TAGGACC9200.011.8733294
CGGTCCA3400.011.73583910
ACCGCAA1302.6157068E-911.692224514
CCGTCGT3350.011.6274069
CGTCGTA3350.011.62740610
Produced by FastQC (version 0.11.5)