##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1872168
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201561505164065	32.0	32.0	32.0	32.0	32.0
2	31.363865315505873	32.0	32.0	32.0	32.0	32.0
3	31.468034385802984	32.0	32.0	32.0	32.0	32.0
4	31.583978040432267	32.0	32.0	32.0	32.0	32.0
5	31.502457578593376	32.0	32.0	32.0	32.0	32.0
6	35.09598070258652	36.0	36.0	36.0	36.0	36.0
7	35.10948483255776	36.0	36.0	36.0	36.0	36.0
8	35.05066158592605	36.0	36.0	36.0	36.0	36.0
9	35.17886909721777	36.0	36.0	36.0	36.0	36.0
10	35.05400102982211	36.0	36.0	36.0	36.0	36.0
11	35.19846829985343	36.0	36.0	36.0	36.0	36.0
12	35.09704310724251	36.0	36.0	36.0	36.0	36.0
13	35.14737993598865	36.0	36.0	36.0	36.0	36.0
14	35.1112245268587	36.0	36.0	36.0	36.0	36.0
15	35.061697988642045	36.0	36.0	36.0	36.0	36.0
16	35.08074702697621	36.0	36.0	36.0	36.0	36.0
17	35.06133530751514	36.0	36.0	36.0	36.0	36.0
18	35.073945286961425	36.0	36.0	36.0	36.0	36.0
19	35.05452448711868	36.0	36.0	36.0	36.0	36.0
20	35.03406638720457	36.0	36.0	36.0	36.0	36.0
21	35.01961095371783	36.0	36.0	36.0	36.0	36.0
22	35.005998393306584	36.0	36.0	36.0	36.0	36.0
23	34.968471846543686	36.0	36.0	36.0	36.0	36.0
24	34.9543262143141	36.0	36.0	36.0	36.0	36.0
25	34.92099587216532	36.0	36.0	36.0	32.0	36.0
26	34.883156853444774	36.0	36.0	36.0	32.0	36.0
27	34.859542519688404	36.0	36.0	36.0	32.0	36.0
28	34.83794456480401	36.0	36.0	36.0	32.0	36.0
29	34.81747738450823	36.0	36.0	36.0	32.0	36.0
30	34.793786134577665	36.0	36.0	36.0	32.0	36.0
31	34.792028813653474	36.0	36.0	36.0	32.0	36.0
32	34.74856316313493	36.0	36.0	36.0	32.0	36.0
33	34.724801940851464	36.0	36.0	36.0	32.0	36.0
34	34.700008225757514	36.0	36.0	36.0	32.0	36.0
35	34.6649387234479	36.0	36.0	36.0	32.0	36.0
36	34.62566874340337	36.0	36.0	36.0	32.0	36.0
37	34.6097251956021	36.0	36.0	36.0	32.0	36.0
38	34.56655065143726	36.0	36.0	36.0	32.0	36.0
39	34.532082056738496	36.0	36.0	36.0	32.0	36.0
40	34.522462727703925	36.0	36.0	36.0	32.0	36.0
41	34.48678110084138	36.0	36.0	36.0	32.0	36.0
42	34.45091679806513	36.0	36.0	36.0	32.0	36.0
43	34.433383649330615	36.0	36.0	36.0	32.0	36.0
44	34.3984204409006	36.0	36.0	36.0	32.0	36.0
45	34.33410569991582	36.0	36.0	36.0	32.0	36.0
46	34.33175975660304	36.0	36.0	36.0	32.0	36.0
47	34.30145424983228	36.0	36.0	36.0	32.0	36.0
48	34.25273266074412	36.0	36.0	36.0	32.0	36.0
49	34.21139128539746	36.0	36.0	36.0	32.0	36.0
50	33.69227441127078	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	17.0
21	53.0
22	177.0
23	505.0
24	1365.0
25	3136.0
26	6578.0
27	12212.0
28	20571.0
29	31426.0
30	44788.0
31	64450.0
32	94572.0
33	152844.0
34	332460.0
35	1107011.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42158474309613	18.58002112306993	11.86684880016796	26.131545333665972
2	15.801632815270947	20.98147325148603	37.39583912004888	25.821054813194138
3	18.41597686518812	25.078238630627663	28.872738771374408	27.6330457328098
4	12.157447997260919	16.225474197998995	36.64348311924028	34.973594685499805
5	13.86536891988326	37.466242345772386	33.74205733673474	14.926331397609616
6	33.37147105859429	36.112847071994835	17.18553202126739	13.330149848143483
7	29.378773699796174	30.927779985556853	21.43632409057307	18.257122224073907
8	27.681009396592614	33.5722007853996	19.86814217527487	18.87864764273292
9	27.595279910777236	14.067327291140538	19.02927515052068	39.30811764756154
10	15.748746907328828	27.141634725088775	32.14433747398738	24.96528089359502
11	36.85166074839438	21.192649377619958	22.971976873870297	18.983713000115372
12	24.493581772575965	24.081332444524207	29.418246653078146	22.00683912982168
13	29.696052918327844	20.002211340007946	25.522121946321057	24.779613795343153
14	23.091677669952695	20.4081044008871	25.602990757239734	30.897227171920466
15	24.675082578059236	28.268029364886054	22.919631144213554	24.137256912841153
16	24.97900829412745	25.898423645741193	25.1826224996902	23.939945560441156
17	23.407942022297142	26.35671585028694	26.138038893945414	24.0973032334705
18	24.088116023775644	24.901664807859124	27.926019459792066	23.08419970857316
19	25.1826224996902	25.440238269215154	25.91920169557433	23.45793753552032
20	25.179524487118677	24.67027531717239	26.29342024861017	23.856779947098765
21	26.00834967802035	24.679248870827834	24.867426427542828	24.444975023608993
22	25.19565551809453	25.475491515718673	25.376408527439846	23.95244443874695
23	23.57202986056807	25.14801022130493	26.262066224825976	25.01789369330103
24	24.25081509779037	25.64780511150709	26.035964721114773	24.06541506958777
25	24.310478546797082	25.165903914605952	26.212391195661926	24.311226342935036
26	23.369911247281227	26.03788762546951	26.787019113669285	23.80518201357998
27	24.52012853547331	25.77087099021028	25.779470645796742	23.929529828519662
28	23.565780421415173	25.47949756645771	26.36649061409019	24.58823139803693
29	23.836055311275484	25.57703154845078	26.138145721965124	24.448767418308613
30	23.9181526444208	25.51031745014336	26.600978117348443	23.970551788087395
31	24.32009306857077	25.264292520756683	25.736899679943253	24.678714730729293
32	23.811004140654042	25.86199529102089	25.809916631413422	24.517083936911643
33	23.41280269719384	25.382444310553325	26.56802167326864	24.636731318984193
34	23.99581661474825	25.69245922374488	26.789956884211247	23.521767277295627
35	24.822558659265624	25.50246559069485	26.164318586793495	23.510657163246034
36	23.52155362125621	25.894791493071136	26.309604693595873	24.27405019207678
37	24.775287260544992	25.606088769811258	25.3911507941595	24.227473175484253
38	23.85811529734511	25.89169348049961	25.852113699197936	24.398077522957344
39	24.492673734408452	25.33084637703454	25.553582798124953	24.622897090432055
40	24.985845287388738	25.35840800611911	26.170728267975953	23.4850184385162
41	23.808920994269744	25.55951175321873	26.702945462159377	23.928621790352146
42	24.81652287615214	26.259075040274166	25.982390469231394	22.942011614342302
43	23.699689344118692	25.34008700073925	26.312809534187103	24.64741412095496
44	23.345554458787888	25.847626922370214	26.173986522577035	24.632832096264863
45	23.594036432627842	26.029020899833778	26.082862221766423	24.29408044577196
46	23.483815003584084	25.254331079615806	26.32864820747733	24.933205709322785
47	24.19889668021246	25.14608731695019	26.387001593874054	24.2680144089633
48	24.100134175992753	26.247484200135883	25.495361527384297	24.157020096487066
49	23.553334957119233	25.831175407335238	26.08473171211131	24.530757923434223
50	23.166362829811657	26.81790673588414	25.422571811168552	24.59315862313565
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	78.5
2	84.0
3	273.5
4	463.0
5	501.5
6	540.0
7	491.5
8	443.0
9	591.5
10	740.0
11	1051.0
12	1362.0
13	2407.5
14	3453.0
15	4570.0
16	5687.0
17	6672.0
18	7657.0
19	8335.5
20	9014.0
21	10511.0
22	12008.0
23	12781.0
24	13554.0
25	15437.0
26	17320.0
27	21280.0
28	25240.0
29	29315.0
30	33390.0
31	36763.0
32	40136.0
33	47230.0
34	54324.0
35	62621.5
36	70919.0
37	78793.0
38	86667.0
39	88946.0
40	91225.0
41	96100.0
42	100975.0
43	101076.5
44	101178.0
45	110543.5
46	119909.0
47	126625.0
48	133341.0
49	134380.5
50	135420.0
51	127717.5
52	120015.0
53	116590.0
54	113165.0
55	114308.0
56	115451.0
57	112751.5
58	110052.0
59	100136.5
60	90221.0
61	79881.0
62	69541.0
63	60933.0
64	52325.0
65	45414.0
66	38503.0
67	34541.5
68	30580.0
69	27921.0
70	25262.0
71	19824.5
72	14387.0
73	12722.0
74	11057.0
75	8191.5
76	5326.0
77	4681.0
78	4036.0
79	3456.0
80	2876.0
81	2303.5
82	1731.0
83	1477.0
84	1223.0
85	1004.5
86	786.0
87	561.0
88	336.0
89	214.5
90	93.0
91	65.0
92	37.0
93	27.0
94	17.0
95	16.0
96	15.0
97	10.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015009336768922446
2	0.0034184966306442586
3	5.875541083919819E-4
4	3.738980689767158E-4
5	0.0
6	3.2048405912289923E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.0682801970763308E-4
47	0.0
48	0.0
49	0.0
50	5.341400985381654E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1872168.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.745392208923164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.00157068583032	42.70226580107824
2	13.50035345624822	14.781642898428004
3	4.037701495323472	6.631366559521171
4	1.631696234613866	3.5731140131903683
5	0.8224968949083964	2.251395755119081
6	0.4604980298167886	1.512608715225389
7	0.2809399614739696	1.076611786463656
8	0.19588434642640212	0.8579012298161569
9	0.1381737391780108	0.6807937989846287
>10	0.749827816721436	7.549730699828965
>50	0.08460185421190086	3.257931886374327
>100	0.08459197488173849	9.838467555665401
>500	0.008919154985383473	3.2604938998650965
>1k	0.0027443553801179917	2.025675400439515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.341400985381654E-5	0.0
11	0.0	0.0	0.0	1.6024202956144962E-4	0.0
12	0.0	0.0	0.0	1.6024202956144962E-4	2.1365603941526616E-4
13	0.0	0.0	0.0	1.6024202956144962E-4	2.1365603941526616E-4
14	0.0	0.0	0.0	2.1365603941526616E-4	2.1365603941526616E-4
15	0.0	0.0	0.0	2.1365603941526616E-4	2.1365603941526616E-4
16	0.0	0.0	0.0	3.2048405912289923E-4	2.1365603941526616E-4
17	0.0	0.0	0.0	3.2048405912289923E-4	2.1365603941526616E-4
18	0.0	0.0	0.0	5.341400985381654E-4	2.1365603941526616E-4
19	0.0	0.0	0.0	6.409681182457985E-4	2.1365603941526616E-4
20	0.0	0.0	0.0	8.546241576610647E-4	2.1365603941526616E-4
21	0.0	0.0	0.0	0.0010148661872225143	2.670700492690827E-4
22	0.0	0.0	0.0	0.0015490062857606796	2.670700492690827E-4
23	5.341400985381654E-5	0.0	0.0	0.003578738660205708	2.670700492690827E-4
24	5.341400985381654E-5	0.0	0.0	0.006463095192311801	2.670700492690827E-4
25	5.341400985381654E-5	0.0	0.0	0.00801210147807248	2.670700492690827E-4
26	5.341400985381654E-5	0.0	0.0	0.01094987202003239	3.2048405912289923E-4
27	5.341400985381654E-5	0.0	0.0	0.014101298601407566	3.738980689767158E-4
28	5.341400985381654E-5	0.0	0.0	0.022273642109041498	3.738980689767158E-4
29	5.341400985381654E-5	0.0	0.0	0.036642010759718145	3.738980689767158E-4
30	5.341400985381654E-5	0.0	0.0	0.06372291375560313	3.738980689767158E-4
31	1.0682801970763308E-4	0.0	0.0	0.12151687241743263	3.738980689767158E-4
32	1.0682801970763308E-4	0.0	0.0	0.18710927651791934	3.738980689767158E-4
33	1.0682801970763308E-4	0.0	0.0	0.2511526743326454	3.738980689767158E-4
34	1.0682801970763308E-4	0.0	0.0	0.32368889971412823	4.2731207883053233E-4
35	1.0682801970763308E-4	0.0	0.0	0.41422564641634724	4.2731207883053233E-4
36	1.0682801970763308E-4	0.0	0.0	0.5373449391293944	4.2731207883053233E-4
37	1.0682801970763308E-4	0.0	0.0	0.7095517068980989	4.2731207883053233E-4
38	1.0682801970763308E-4	0.0	0.0	0.9400331594173172	4.2731207883053233E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAC	90	4.084468E-7	21.999895	12
TAGGACG	1340	0.0	19.701397	4
TGTAGGA	1595	0.0	19.173346	2
GTAGGAC	1695	0.0	18.690174	3
AGGACGT	1425	0.0	18.680613	5
GTCCTAC	1395	0.0	18.454481	1
CTGTAGG	1650	0.0	18.269505	1
GGACGTG	1425	0.0	18.06307	6
ACGCGAA	110	2.7982187E-6	17.999912	11
AACCGGT	210	0.0	17.809437	7
GTCCTAA	805	0.0	17.766735	1
CGTCTTA	285	0.0	17.7543	15
GTAAGAC	150	9.606083E-9	17.599915	3
TAGGACA	565	0.0	17.52204	4
CGTGGAA	865	0.0	17.294714	9
ATACGAA	560	0.0	17.285631	40
ACGTGGA	880	0.0	16.999916	8
GTATACC	130	7.322451E-7	16.922995	3
GACGTGG	905	0.0	16.773401	7
TTAGGAC	840	0.0	16.761824	3
>>END_MODULE