FastQCFastQC Report
Thu 2 Feb 2017
SRR4062410_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062410_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475613
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG103402.174036454007775No Hit
TCCATGTACTCTGCGTTGATACCAC87181.8330028825957239No Hit
GAGTACATGGAAGCAGTGGTATCAA46610.979998444113176No Hit
CATGTACTCTGCGTTGATACCACTG40850.858891577816418No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28110.5910267381253246No Hit
GTATCAACGCAGAGTACTTTTTTTT27680.5819857741483097No Hit
CCCATGTACTCTGCGTTGATACCAC27370.5754678698858106No Hit
GCTTCCATGTACTCTGCGTTGATAC27150.5708422604091983No Hit
GTACATGGGAAGCAGTGGTATCAAC25330.532575854739042No Hit
GCGTTGATACCACTGCTTCCATGTA24760.520591321095092No Hit
CATGGAAGCAGTGGTATCAACGCAG22360.47013012680477617No Hit
TATCAACGCAGAGTACTTTTTTTTT21480.45162768889832705No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19690.41399204815679974No Hit
GTATCAACGCAGAGTACATGGAAGC19040.4003254747031725No Hit
ACTCTGCGTTGATACCACTGCTTCC17030.358064224485033No Hit
CAGTGGTATCAACGCAGAGTACATG15670.3294695477205207No Hit
ACGCAGAGTACATGGAAGCAGTGGT15610.3282080178632628No Hit
GTCCTACAGTGGACATTTCTAAATT15280.32126960364834434No Hit
GTCCTAAAGTGTGTATTTCTCATTT14230.29919283114633116No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14190.29835181124149257No Hit
CTGTAGGACGTGGAATATGGCAAGA12760.2682853496435127No Hit
GTACATGGGGTGGTATCAACGCAAA12430.2613469354285942No Hit
GTCCTACAGTGTGCATTTCTCATTT11980.25188546149916No Hit
GTATCAACGCAGAGTACATGGGAAG11940.25104444159432143No Hit
CTTTAGGACGTGAAATATGGCGAGG11880.24978291173706355No Hit
TATCAACGCAGAGTACATGGAAGCA10830.22770613923505037No Hit
GGTATCAACGCAGAGTACTTTTTTT10010.21046523118585908No Hit
GAGTACATGGGAAGCAGTGGTATCA9310.19574738285118362No Hit
CTGTAGGACCTGGAATATGGCGAGA9280.1951166179225547No Hit
CCATAGGGTCTTCTCGTCTTATTAT8900.187126928826588No Hit
GCGTTGATACCACTGCTTCCCATGT8810.18523463404070115No Hit
GTATTAGAGGCACTGCCTGCCCAGT8700.18292182930239503No Hit
ATACCACTGCTTCCATGTACTCTGC8180.17198857053949324No Hit
GGTATCAACGCAGAGTACATGGAAG7990.1679937259915099No Hit
AAGCAGTGGTATCAACGCAGAGTAC7950.1671527060866713No Hit
AAAAAGTACTCTGCGTTGATACCAC7830.16462964637215552No Hit
GATATACACTGTTCTACAAATCCCG7600.15979378191933358No Hit
TTGTAGAACAGTGTATATCAATGAG7580.15937327196691428No Hit
CTCTAATACTTGTAATGCTAGAGGT7550.15874250703828532No Hit
TATCAACGCAGAGTACATGGGAAGC7130.14991179803748006No Hit
GTACTCTGCGTTGATACCACTGCTT7120.1497015430612704No Hit
GTACATGGGTGGTATCAACGCAAAA7110.14949128808506074No Hit
GTGTATATCAATGAGTTACAATGAG6710.14108108903667477No Hit
GGTATCAACGCAGAGTACATGGGAA6670.14024006913183618No Hit
GCTTCCCATGTACTCTGCGTTGATA6660.14002981415562654No Hit
GTGTATATCAATGAGTTACAATGAA6550.13771700941732037No Hit
ATCTATAACTTTATAGATGCAACAC6330.1330913999407081No Hit
CTGAAGGACCTGGAATATGGCGAGA6310.1326708899882888No Hit
CCACTGCTTCCATGTACTCTGCGTT6300.13246063501207914No Hit
GTCCTTCAGTGTGCATTTCTCATTT6200.13035808524998266No Hit
ACATGGAAGCAGTGGTATCAACGCA6190.130147830273773No Hit
GTGGTATCAACGCAGAGTACATGGA6140.12909655539272477No Hit
GTACATGGGAGTGGTATCAACGCAA6120.12867604544030545No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6080.12783502553546686No Hit
CCATTGGGATGTCCTGATCCAACAT6010.12636324070199934No Hit
CTTCTACACCATTGGGATGTCCTGA5970.12552222079716072No Hit
ACGCAGAGTACATGGGAAGCAGTGG5620.118163296629823No Hit
GAAGCAGTGGTATCAACGCAGAGTA5620.118163296629823No Hit
CTGTTAGTATGAGTAACAAGAATTC5610.11795304165361332No Hit
ATTTAGAAATGTCCACTGTAGGACG5280.11101462743869492No Hit
ATCCTGACCGTGCAAAGGTAGCATA5160.10849156772417912No Hit
CTGCTTCCATGTACTCTGCGTTGAT5160.10849156772417912No Hit
GATATATTTTGATCAACGGACCAAG5140.10807105777175981No Hit
GTCAGGATACCGCGGCCGTTAAACT4990.10491723312861508No Hit
TGGAAGCAGTGGTATCAACGCAGAG4760.10008136867579312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAG307.703327E-419.00315719
GTTTAAA401.2849638E-518.995161
TCCAACG1100.015.548038518
CGGTTGT551.1254941E-515.544764510
TAGGACC3850.015.2964124
ACTCGGC508.696945E-515.199326511
TAGAAAT1950.015.1003054
AATCGTA953.0195224E-1015.00091513
CCAACGA902.2664608E-914.78023419
GACAGCC456.746988E-414.7786797
GAGGCAC902.2700988E-914.7786797
CGTGCGC456.752377E-414.777122510
TCTAGTA456.763165E-414.7740132
GTACTAC456.763165E-414.7740131
TTAGAAA2000.014.7212493
GTCCTAT653.3691285E-614.6116611
CCCAATT1058.731149E-1114.47707413
AACTTTT400.00526615214.2523715
GGACCGT400.005269832414.250876
AAATCGT1006.548362E-1014.24936912
Produced by FastQC (version 0.11.5)