##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062410_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 475613 Sequences flagged as poor quality 0 Sequence length 50 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89885894624411 32.0 32.0 32.0 32.0 32.0 2 31.358297607508625 32.0 32.0 32.0 32.0 32.0 3 31.487280625214197 32.0 32.0 32.0 32.0 32.0 4 31.62006295033988 32.0 32.0 32.0 32.0 32.0 5 31.547070832798934 32.0 32.0 32.0 32.0 32.0 6 35.13067977536358 36.0 36.0 36.0 36.0 36.0 7 35.15722236356029 36.0 36.0 36.0 36.0 36.0 8 35.10563210004773 36.0 36.0 36.0 36.0 36.0 9 35.19966653560773 36.0 36.0 36.0 36.0 36.0 10 35.107595881525526 36.0 36.0 36.0 36.0 36.0 11 35.21891327612996 36.0 36.0 36.0 36.0 36.0 12 35.13315657898333 36.0 36.0 36.0 36.0 36.0 13 35.14627648949882 36.0 36.0 36.0 36.0 36.0 14 35.11622684829893 36.0 36.0 36.0 36.0 36.0 15 35.09074815028185 36.0 36.0 36.0 36.0 36.0 16 35.09018046184608 36.0 36.0 36.0 36.0 36.0 17 35.06596960133554 36.0 36.0 36.0 36.0 36.0 18 35.077798546297096 36.0 36.0 36.0 36.0 36.0 19 35.010371877976425 36.0 36.0 36.0 36.0 36.0 20 34.98128310201782 36.0 36.0 36.0 36.0 36.0 21 34.883426230990324 36.0 36.0 36.0 32.0 36.0 22 34.83432538639608 36.0 36.0 36.0 32.0 36.0 23 34.77683326570131 36.0 36.0 36.0 32.0 36.0 24 34.74401036136523 36.0 36.0 36.0 32.0 36.0 25 34.732364338232976 36.0 36.0 36.0 32.0 36.0 26 34.64029788925029 36.0 36.0 36.0 32.0 36.0 27 34.56970688353766 36.0 36.0 36.0 32.0 36.0 28 34.494969649694184 36.0 36.0 36.0 32.0 36.0 29 34.420331235689524 36.0 36.0 36.0 32.0 36.0 30 34.37826552259926 36.0 36.0 36.0 32.0 36.0 31 34.32638720976929 36.0 36.0 36.0 32.0 36.0 32 34.269027549709534 36.0 36.0 36.0 32.0 36.0 33 34.21396597653975 36.0 36.0 36.0 32.0 36.0 34 34.13632301892505 36.0 36.0 36.0 32.0 36.0 35 33.99035980934079 36.0 36.0 36.0 32.0 36.0 36 33.906526945226474 36.0 36.0 36.0 32.0 36.0 37 33.74531394221773 36.0 36.0 36.0 32.0 36.0 38 33.630958363207064 36.0 36.0 36.0 27.0 36.0 39 33.3362103222578 36.0 36.0 36.0 14.0 36.0 40 33.20447296436389 36.0 36.0 36.0 14.0 36.0 41 33.22560148692319 36.0 36.0 36.0 14.0 36.0 42 32.92296047416702 36.0 36.0 36.0 14.0 36.0 43 33.029420978821015 36.0 36.0 36.0 14.0 36.0 44 32.98945150784356 36.0 36.0 36.0 14.0 36.0 45 32.628803249700916 36.0 36.0 36.0 14.0 36.0 46 32.79015502099396 36.0 36.0 36.0 14.0 36.0 47 32.601015952045046 36.0 36.0 36.0 14.0 36.0 48 32.6132717146083 36.0 36.0 36.0 14.0 36.0 49 32.665068027997556 36.0 36.0 36.0 14.0 36.0 50 32.06142178620013 36.0 36.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 5.0 21 17.0 22 66.0 23 215.0 24 655.0 25 1658.0 26 3604.0 27 6882.0 28 11094.0 29 15042.0 30 19398.0 31 24829.0 32 34228.0 33 41048.0 34 76066.0 35 240806.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22748329818083 20.080244434304078 13.076730586040917 23.615541681474177 2 11.587096665671428 22.80875865752169 42.049167574295936 23.554977102510943 3 19.05657997098463 28.402472614116608 30.07422047475873 22.46672694014003 4 10.63537218441121 18.31055211086371 38.57375039685794 32.48032530786714 5 10.121043789803895 40.973858998807856 36.24985019332945 12.655247018058798 6 29.605745920476362 37.630228318758974 19.518425927288707 13.245599833475957 7 25.39796010622081 33.775149123341876 23.125944833299343 17.70094593713797 8 25.827090512664707 36.91909178260476 22.012644734269248 15.241172970461278 9 26.41979929059971 15.985685841219647 20.431106803220263 37.16340806496038 10 14.923687956384708 29.138606387966686 34.93848990670987 20.999215748938738 11 33.206829922647195 22.880367021086474 24.62485255869793 19.287950497568403 12 22.404139499971613 26.538172842205743 32.70011542998194 18.357572227840702 13 30.473304976945542 22.433154686688546 26.957841774720205 20.135698561645707 14 21.620939713590673 23.472024524140426 26.881098708403684 28.025937053865224 15 23.907252324894397 30.807610389118885 27.25240899638992 18.032728289596793 16 22.455862224119187 27.382136316711275 30.951635047822496 19.210366411347042 17 20.08082201285499 28.39892938166114 31.510072264635326 20.01017634084855 18 21.467453580957628 25.069541833381344 35.41072258327674 18.052282002384292 19 22.231940674455913 30.296480541953226 27.636965347877375 19.83461343571349 20 21.257829369676607 26.987487726365767 33.6056836125169 18.148999291440727 21 21.480279134506418 27.835025535466862 26.089278467998138 24.595416862028582 22 20.22442616160618 31.361001486502683 27.519853326128597 20.89471902576254 23 19.374996057719194 29.73783307016419 28.165125848116013 22.722045024000607 24 20.706120312102485 31.51448761913573 28.216848572263586 19.562543496498204 25 23.04247360774411 28.159028453805927 27.608160416136652 21.19033752231331 26 17.42845548797026 28.6791992649486 31.191746230653916 22.700599016427223 27 21.583724582801565 30.784902851688244 29.577618778292436 18.053753787217758 28 19.81611099780704 27.6441140170685 29.045253178529602 23.494521806594857 29 19.519230971399015 29.375143236202543 30.126594521175832 20.979031271222613 30 22.456282734071607 27.543191628487868 30.411700268916114 19.58882536852441 31 19.909464207244124 27.36174158401894 29.798596758288774 22.93019745044816 32 18.22742439756693 30.40497210967741 30.43167449165603 20.935929001099634 33 18.74864648359065 29.28115926183683 30.84587679478904 21.12431745978348 34 17.928652076373016 30.782590046949938 32.748474074510156 18.540283802166886 35 21.149968566881057 30.22919894956614 29.671392497681936 18.949439985870868 36 19.59429199790586 29.96427767954198 30.621114225220925 19.820316097331233 37 21.157117236072185 29.454199107257374 28.667635241257074 20.72104841541337 38 20.47652187808155 31.017865365328532 27.160317316810094 21.34529543977982 39 20.19667250474651 28.703799097165135 29.632285072106946 21.467243325981418 40 22.87595166658607 29.207570020163452 28.085439212132552 19.831039101117927 41 20.00218665175258 27.93678894395233 31.16945920317569 20.8915652011194 42 22.21154594176358 30.844405009955572 29.111062986083226 17.832986062197627 43 19.901684773124366 29.223128888402965 28.552625769270396 22.32256056920227 44 19.57074344057038 29.196426506424338 31.436062513009528 19.796767539995752 45 20.84783216606779 29.360845897820287 29.278636202122314 20.512685733989606 46 20.531862106086475 27.654474655811878 30.338595325601542 21.475067912500105 47 23.069014238496926 27.23669713968529 30.004919976787804 19.689368645029983 48 19.13845920948334 32.768448297250075 26.428209489648097 21.66488300361849 49 19.46813901218007 28.112772359039806 32.286123381825135 20.13296524695498 50 17.974315198102655 33.52312389090267 25.659150736314473 22.8434101746802 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13.0 1 11.0 2 9.0 3 85.0 4 161.0 5 171.5 6 182.0 7 191.5 8 201.0 9 264.0 10 327.0 11 490.5 12 654.0 13 1089.0 14 1524.0 15 2188.5 16 2853.0 17 3560.5 18 4268.0 19 4716.0 20 5164.0 21 6056.5 22 6949.0 23 6912.5 24 6876.0 25 7530.5 26 8185.0 27 10931.0 28 13677.0 29 15530.5 30 17384.0 31 18273.5 32 19163.0 33 22892.5 34 26622.0 35 30536.0 36 34450.0 37 36308.0 38 38166.0 39 35707.5 40 33249.0 41 32634.5 42 32020.0 43 30245.0 44 28470.0 45 32578.5 46 36687.0 47 40092.5 48 43498.0 49 37783.5 50 32069.0 51 26028.0 52 19987.0 53 15707.5 54 11428.0 55 10449.0 56 9470.0 57 9258.0 58 9046.0 59 8374.5 60 7703.0 61 6809.5 62 5916.0 63 5278.0 64 4640.0 65 4363.5 66 4087.0 67 4029.0 68 3971.0 69 3237.5 70 2504.0 71 2187.0 72 1870.0 73 1509.0 74 1148.0 75 773.5 76 399.0 77 320.0 78 241.0 79 208.5 80 176.0 81 137.0 82 98.0 83 75.0 84 52.0 85 40.0 86 28.0 87 23.0 88 18.0 89 12.0 90 6.0 91 4.5 92 3.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013876828429836865 2 0.0031538246431447414 3 6.307649286289484E-4 4 4.205099524192989E-4 5 0.0 6 0.0012615298572578968 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 2.1025497620964946E-4 47 2.1025497620964946E-4 48 0.0 49 0.0 50 2.1025497620964946E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 475613.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.03907413810509 #Duplication Level Percentage of deduplicated Percentage of total 1 78.34431925422216 35.285556031902864 2 11.917224515972732 10.734815169906792 3 3.7311983579704244 5.041491584058179 4 1.722038719786846 3.1023611827666944 5 0.9182883843892917 2.067942931233503 6 0.5838648640574038 1.5778039739349639 7 0.3973791679083664 1.25283128630544 8 0.3074718926687416 1.1078599495432755 9 0.23735733079204513 0.9621318976889089 >10 1.4756062737290492 13.068978832808794 >50 0.19483195884162477 6.082273328416954 >100 0.15586556707315632 13.458390929259265 >500 0.01173686499044852 3.452506827961874 >1k 0.0028168475977076446 2.8050560742125317 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 3966 0.8338712356474697 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 3834 0.8061175787877959 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 1700 0.35743345955640404 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 1618 0.3401925515072128 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1114 0.23422404349754947 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 1034 0.21740364540077753 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 945 0.19869095251811875 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 931 0.19574738285118362 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 889 0.18691667385037836 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 887 0.18649616389795906 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 883 0.18565514399312047 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 857 0.18018851461166957 No Hit GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT 805 0.1692552558487678 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 659 0.13855802932215897 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 654 0.13750675444111074 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 648 0.13624522458385285 No Hit CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT 630 0.13246063501207914 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 614 0.12909655539272477 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 561 0.11795304165361332 No Hit CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA 561 0.11795304165361332 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 555 0.11669151179635544 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 547 0.11500947198667824 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 536 0.11269666724837209 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 535 0.11248641227216245 No Hit GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 535 0.11248641227216245 No Hit ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT 528 0.11101462743869492 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA 524 0.11017360753385631 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 521 0.10954284260522736 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 516 0.10849156772417912 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 506 0.10638901796208261 No Hit GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC 501 0.10533774308103437 No Hit GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC 481 0.10113264355684139 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 476 0.10008136867579312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 2.1025497620964944E-4 0.0 15 0.0 0.0 0.0 2.1025497620964944E-4 0.0 16 0.0 0.0 0.0 4.2050995241929887E-4 0.0 17 0.0 0.0 0.0 6.307649286289483E-4 0.0 18 0.0 0.0 0.0 6.307649286289483E-4 0.0 19 0.0 0.0 0.0 0.0014717848334675461 0.0 20 0.0 0.0 0.0 0.0021025497620964944 0.0 21 0.0 0.0 0.0 0.0029435696669350923 0.0 22 0.0 0.0 0.0 0.006307649286289484 0.0 23 0.0 0.0 0.0 0.01135376871532107 0.0 24 0.0 0.0 0.0 0.023548557335480738 0.0 25 0.0 0.0 0.0 0.029015186716931624 0.0 26 0.0 0.0 0.0 0.03889717059878515 0.0 27 0.0 0.0 0.0 0.050881704242735165 0.0 28 0.0 0.0 0.0 0.07295847674474835 0.0 29 2.1025497620964944E-4 0.0 0.0 0.10071213360442208 0.0 30 2.1025497620964944E-4 0.0 0.0 0.1419221089415134 0.0 31 2.1025497620964944E-4 0.0 0.0 0.20773191649513364 0.0 32 2.1025497620964944E-4 0.0 0.0 0.29961334109875043 0.0 33 2.1025497620964944E-4 0.0 0.0 0.374253857653176 0.0 34 2.1025497620964944E-4 0.0 0.0 0.48043262063904896 0.0 35 2.1025497620964944E-4 0.0 0.0 0.5981754073164527 0.0 36 2.1025497620964944E-4 0.0 0.0 0.7520820499019161 0.0 37 2.1025497620964944E-4 0.0 0.0 0.9989213919720445 0.0 38 2.1025497620964944E-4 0.0 0.0 1.2497555785901562 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCATA 20 7.8522833E-4 43.999477 11 ACGCACT 25 0.0023515008 35.199577 40 TATAAGG 45 1.0347048E-6 34.22541 2 TATTGAT 30 0.00573894 29.336067 2 CGTTCTA 30 0.00574189 29.332983 12 TAGGACC 465 0.0 26.970253 4 CCTAATA 50 8.3257095E-5 26.402458 2 GTCTTAC 45 0.001395355 24.449293 1 TATGTAC 45 0.0013962102 24.446722 3 CGGCTTG 120 2.3646862E-11 23.833048 26 CTGTAGG 970 0.0 23.819157 1 CGGACCC 75 2.060102E-6 23.466387 14 AGTAAGT 85 2.3466964E-7 23.29384 36 CGCACCG 95 2.6770067E-8 23.157618 9 TGTAGGA 1055 0.0 23.149076 2 TAGGACA 230 0.0 22.958662 4 CTATACT 70 3.2077583E-5 22.00205 4 TAAGGCT 60 2.867423E-4 22.00205 4 ACCGGAC 80 3.6082874E-6 21.999739 12 AACTTCA 60 2.8694628E-4 21.999737 40 >>END_MODULE