FastQCFastQC Report
Thu 2 Feb 2017
SRR4062409_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062409_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912888
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG141451.5494781397060757No Hit
TCCATGTACTCTGCGTTGATACCAC120671.3218489015081805No Hit
GAGTACATGGAAGCAGTGGTATCAA68170.7467509705462225No Hit
CATGTACTCTGCGTTGATACCACTG60600.6638273260246602No Hit
GCTTCCATGTACTCTGCGTTGATAC37670.41264645827308494No Hit
GTATCAACGCAGAGTACTTTTTTTT36640.40136358457992655No Hit
CCCATGTACTCTGCGTTGATACCAC34730.38044097413921535No Hit
GCGTTGATACCACTGCTTCCATGTA33440.3663099964070072No Hit
GTACATGGGAAGCAGTGGTATCAAC32150.3521790186747991No Hit
CATGGAAGCAGTGGTATCAACGCAG31720.34746869276406306No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26860.29423105572644176No Hit
GTATCAACGCAGAGTACATGGAAGC26050.2853581162201716No Hit
TATCAACGCAGAGTACTTTTTTTTT25660.28108596016159704No Hit
ACTCTGCGTTGATACCACTGCTTCC22990.25183812252981747No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22690.2485518486386063No Hit
ACGCAGAGTACATGGAAGCAGTGGT22560.2471277966190814No Hit
CAGTGGTATCAACGCAGAGTACATG21890.23978845159537643No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16820.18425042283390733No Hit
TATCAACGCAGAGTACATGGAAGCA16750.18348362559262474No Hit
GTATCAACGCAGAGTACATGGGAAG15360.16825722323001288No Hit
GGTATCAACGCAGAGTACTTTTTTT13300.14569147584369604No Hit
GTACATGGGGTGGTATCAACGCAAA12970.14207657456336373No Hit
GGTATCAACGCAGAGTACATGGAAG12570.13769487604174882No Hit
ATACCACTGCTTCCATGTACTCTGC12170.1333131775201339No Hit
GAGTACATGGGAAGCAGTGGTATCA11970.13112232825932643No Hit
GCGCAAGACGGACCAGAGCGAAAGC11620.1272883420529134No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11540.1264120023485904No Hit
AAGCAGTGGTATCAACGCAGAGTAC11010.12060625180745065No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9990.10943292057733259No Hit
GCGTTGATACCACTGCTTCCCATGT9940.10888520826213073No Hit
GAATAACGCCGCCGCATCGCCAGTC9460.10362717003619282No Hit
GAATAGGACCGCGGTTCTATTTTGT9340.10231266047970836No Hit
GTGGTATCAACGCAGAGTACATGGA9320.10209357555362761No Hit
GAACTACGACGGTATCTGATCGTCT9270.10154586323842575No Hit
GGGTAGGCACACGCTGAGCCAGTCA9240.10121723584930462No Hit
GTACTCTGCGTTGATACCACTGCTT9140.10012181121890089No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC250.006040976418.9941313
CGAAATT453.5340185E-516.89015813
CGCGCGC453.5340185E-516.89015814
CGCGCTA402.756811E-416.62716116
CGGTCCA2050.015.29467110
GGGCGTA508.697992E-515.20197616
GCGAAAT508.702064E-515.2011412
ATCGACG456.751875E-414.77969816
AGGATTA456.7546853E-414.7788885
TATCCTC456.7546853E-414.7788885
GGTCCAA2200.014.682116511
CTTACAC653.3792458E-614.6108693
AGAATTT2250.014.35742117
TTTCGTT801.2843702E-714.25185217
TAGAGTG1800.014.251075
TAACGAA602.5641466E-514.2510713
CGACTAA400.00527425914.2510713
ACCGACC551.9565935E-413.8199788
GCGTTAC551.9575044E-413.81922113
GGCCTAT1101.8735591E-1013.8070981
Produced by FastQC (version 0.11.5)