##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062409_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	912888
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.086375327531965	32.0	32.0	32.0	32.0	32.0
2	30.876964096362315	32.0	32.0	32.0	32.0	32.0
3	30.877947787680416	32.0	32.0	32.0	32.0	32.0
4	30.893351648833153	32.0	32.0	32.0	32.0	32.0
5	30.77408948304721	32.0	32.0	32.0	32.0	32.0
6	34.37620934879197	36.0	36.0	36.0	32.0	36.0
7	34.22264176985567	36.0	36.0	36.0	32.0	36.0
8	34.22178514779469	36.0	36.0	36.0	32.0	36.0
9	34.33165185652566	36.0	36.0	36.0	32.0	36.0
10	34.107542217665255	36.0	36.0	36.0	32.0	36.0
11	34.37890409338276	36.0	36.0	36.0	32.0	36.0
12	34.19140245024581	36.0	36.0	36.0	32.0	36.0
13	34.27422422027675	36.0	36.0	36.0	32.0	36.0
14	34.19729035763423	36.0	36.0	36.0	32.0	36.0
15	34.168353620597486	36.0	36.0	36.0	32.0	36.0
16	34.1392459973184	36.0	36.0	36.0	32.0	36.0
17	34.056414368465795	36.0	36.0	36.0	32.0	36.0
18	34.077510055998104	36.0	36.0	36.0	32.0	36.0
19	34.05800163875525	36.0	36.0	36.0	32.0	36.0
20	34.04319259317682	36.0	36.0	36.0	32.0	36.0
21	33.96020431860206	36.0	36.0	36.0	32.0	36.0
22	33.92137480172814	36.0	36.0	36.0	32.0	36.0
23	33.84777322081131	36.0	36.0	36.0	32.0	36.0
24	33.82705764562575	36.0	36.0	36.0	32.0	36.0
25	33.271227138487966	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	27.0
5	95.0
6	136.0
7	33.0
8	134.0
9	178.0
10	106.0
11	34.0
12	60.0
13	53.0
14	200.0
15	276.0
16	409.0
17	608.0
18	834.0
19	1154.0
20	1669.0
21	2186.0
22	2972.0
23	4025.0
24	5324.0
25	7215.0
26	9729.0
27	12642.0
28	17511.0
29	23858.0
30	31625.0
31	44177.0
32	65176.0
33	95432.0
34	210508.0
35	374496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.96272048746339	16.565057016433922	11.433961396001564	25.038261100101117
2	15.594210709661304	20.61218721860355	36.412344526750054	27.38125754498509
3	20.152159981596203	24.699322455373526	27.6895016185484	27.459015944481873
4	12.63813857077592	15.451142193849213	34.84504706051201	37.06567217486286
5	13.803313251031692	37.00363147040582	33.6678070258104	15.525248252752094
6	34.43096514010806	33.868450756704036	17.288468071582777	14.412116031605132
7	29.42513112534871	30.868523887558304	20.863974920393076	18.842370066699914
8	29.390656640131212	31.636650283462686	20.393635437714405	18.579057638691697
9	27.483325712995033	14.91426297074777	18.24780462987633	39.35460668638087
10	16.32699613100199	26.994705350797926	31.506562382109614	25.171736136090473
11	37.379475526622855	21.596033549451224	20.289456137426413	20.735034786499504
12	23.43331154330439	24.461025827765177	28.88721685758534	23.21844577134509
13	30.83836439706745	19.411516337933946	24.825873102041488	24.924246162957115
14	24.380279944682247	19.466936017187546	24.338166508192927	31.814617529937276
15	26.333029568291757	25.463625507051844	24.724939109913596	23.478405814742803
16	26.675900307385923	25.383848585838315	24.58550055215417	23.35475055462159
17	23.86955615753282	25.459251379125035	26.697009245347164	23.974183217994977
18	25.789041327263096	23.24684597338586	28.338036634198723	22.626076065152326
19	24.75291614339693	27.67295321557251	24.91228262598233	22.661848015048232
20	24.02400854534009	23.32103588121626	28.644107202782937	24.010848370660707
21	25.922509730976472	25.09040019566145	23.5588887219381	25.428201351423972
22	23.800251649047212	25.801706255135276	25.1000727303044	25.29796936551311
23	22.692726608628124	25.2114472744663	25.372795447590363	26.723030669315218
24	22.929753267194243	27.678715388032444	24.86061982088701	24.530911523886303
25	25.199561283246503	24.73890869207568	24.125253633123116	25.936276391554703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157.0
1	157.0
2	435.5
3	714.0
4	714.0
5	714.0
6	2184.5
7	3655.0
8	3655.0
9	3655.0
10	3654.0
11	3653.0
12	3653.0
13	3653.0
14	2990.5
15	2328.0
16	2328.0
17	2328.0
18	3651.0
19	4974.0
20	4974.0
21	4974.0
22	8260.0
23	11546.0
24	11546.0
25	11546.0
26	17228.5
27	22911.0
28	22911.0
29	22911.0
30	28576.5
31	34242.0
32	34242.0
33	34242.0
34	40641.5
35	47041.0
36	47041.0
37	47041.0
38	54756.0
39	62471.0
40	62471.0
41	62471.0
42	81543.0
43	100615.0
44	100615.0
45	100615.0
46	137809.5
47	175004.0
48	175004.0
49	175004.0
50	152727.0
51	130450.0
52	130450.0
53	130450.0
54	112356.5
55	94263.0
56	94263.0
57	94263.0
58	86207.0
59	78151.0
60	78151.0
61	78151.0
62	69290.0
63	60429.0
64	60429.0
65	60429.0
66	49450.5
67	38472.0
68	38472.0
69	38472.0
70	28808.0
71	19144.0
72	19144.0
73	19144.0
74	14912.0
75	10680.0
76	10680.0
77	10680.0
78	8872.0
79	7064.0
80	7064.0
81	7064.0
82	4825.0
83	2586.0
84	2586.0
85	2586.0
86	1965.5
87	1345.0
88	1345.0
89	1345.0
90	904.5
91	464.0
92	464.0
93	464.0
94	276.0
95	88.0
96	88.0
97	88.0
98	264.5
99	441.0
100	441.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00887293950627021
2	0.0032862738912111888
3	0.003614901280332308
4	0.013911892806127367
5	0.03395816354251562
6	0.04140705102926098
7	0.06495868058294117
8	0.08489540885628905
9	0.09365880589951889
10	0.11217148215334193
11	0.1139241615619879
12	0.12663108727467115
13	0.11534821358151273
14	0.11666272313799722
15	0.10943292057733259
16	0.11009017535557485
17	0.11808677515752206
18	0.12235893121609662
19	0.12630245988555003
20	0.11458141634023013
21	0.12148259151177362
22	0.14251474441552525
23	0.1309032433332457
24	0.1284933091463575
25	0.12465932293994443
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	912888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.757846852976165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0173465748913	31.390612167220084
2	13.317631916429502	10.855960041882822
3	4.074941139003789	4.98257480635226
4	1.697225893262425	2.767010921299823
5	0.8762731202590199	1.7857502818448348
6	0.5451677515854788	1.3331918236981377
7	0.3679425533665531	1.0497582368564888
8	0.2670325486721245	0.8706937378830681
9	0.1880944390091352	0.6899691905127718
>10	1.2637908098420514	10.380743832331264
>50	0.1863522539019271	5.416161687373345
>100	0.17477234898798905	13.832569402322717
>500	0.015888395362468027	4.634783880837946
>1k	0.006463076079648012	5.719859151303855
>5k	5.38589673304001E-4	1.4133637727887427
>10k+	5.38589673304001E-4	2.8769970654918477
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	14145	1.5494781397060757	No Hit
TCCATGTACTCTGCGTTGATACCAC	12067	1.3218489015081805	No Hit
GAGTACATGGAAGCAGTGGTATCAA	6817	0.7467509705462225	No Hit
CATGTACTCTGCGTTGATACCACTG	6060	0.6638273260246602	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3767	0.41264645827308494	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3664	0.40136358457992655	No Hit
CCCATGTACTCTGCGTTGATACCAC	3473	0.38044097413921535	No Hit
GCGTTGATACCACTGCTTCCATGTA	3344	0.3663099964070072	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3215	0.3521790186747991	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3172	0.34746869276406306	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2686	0.29423105572644176	No Hit
GTATCAACGCAGAGTACATGGAAGC	2605	0.2853581162201716	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2566	0.28108596016159704	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2299	0.25183812252981747	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2269	0.2485518486386063	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2256	0.2471277966190814	No Hit
CAGTGGTATCAACGCAGAGTACATG	2189	0.23978845159537643	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1682	0.18425042283390733	No Hit
TATCAACGCAGAGTACATGGAAGCA	1675	0.18348362559262474	No Hit
GTATCAACGCAGAGTACATGGGAAG	1536	0.16825722323001288	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1330	0.14569147584369604	No Hit
GTACATGGGGTGGTATCAACGCAAA	1297	0.14207657456336373	No Hit
GGTATCAACGCAGAGTACATGGAAG	1257	0.13769487604174882	No Hit
ATACCACTGCTTCCATGTACTCTGC	1217	0.1333131775201339	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1197	0.13112232825932643	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1162	0.1272883420529134	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1154	0.1264120023485904	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1101	0.12060625180745065	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	999	0.10943292057733259	No Hit
GCGTTGATACCACTGCTTCCCATGT	994	0.10888520826213073	No Hit
GAATAACGCCGCCGCATCGCCAGTC	946	0.10362717003619282	No Hit
GAATAGGACCGCGGTTCTATTTTGT	934	0.10231266047970836	No Hit
GTGGTATCAACGCAGAGTACATGGA	932	0.10209357555362761	No Hit
GAACTACGACGGTATCTGATCGTCT	927	0.10154586323842575	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	924	0.10121723584930462	No Hit
GTACTCTGCGTTGATACCACTGCTT	914	0.10012181121890089	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1908492608074595E-4
2	0.0	0.0	0.0	0.0	2.1908492608074595E-4
3	0.0	0.0	0.0	0.0	2.1908492608074595E-4
4	0.0	0.0	0.0	0.0	2.1908492608074595E-4
5	0.0	0.0	0.0	0.0	2.1908492608074595E-4
6	0.0	0.0	0.0	0.0	3.286273891211189E-4
7	0.0	0.0	0.0	0.0	3.286273891211189E-4
8	0.0	0.0	0.0	0.0	3.286273891211189E-4
9	0.0	0.0	0.0	0.0	3.286273891211189E-4
10	0.0	0.0	0.0	1.0954246304037297E-4	3.286273891211189E-4
11	0.0	0.0	0.0	1.0954246304037297E-4	3.286273891211189E-4
12	0.0	0.0	0.0	1.0954246304037297E-4	7.667972412826108E-4
13	0.0	0.0	0.0	1.0954246304037297E-4	9.858821673633567E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	25	0.0060409764	18.994131	3
CGAAATT	45	3.5340185E-5	16.890158	13
CGCGCGC	45	3.5340185E-5	16.890158	14
CGCGCTA	40	2.756811E-4	16.627161	16
CGGTCCA	205	0.0	15.294671	10
GGGCGTA	50	8.697992E-5	15.201976	16
GCGAAAT	50	8.702064E-5	15.20114	12
ATCGACG	45	6.751875E-4	14.779698	16
AGGATTA	45	6.7546853E-4	14.778888	5
TATCCTC	45	6.7546853E-4	14.778888	5
GGTCCAA	220	0.0	14.6821165	11
CTTACAC	65	3.3792458E-6	14.610869	3
AGAATTT	225	0.0	14.357421	17
TTTCGTT	80	1.2843702E-7	14.251852	17
TAGAGTG	180	0.0	14.25107	5
TAACGAA	60	2.5641466E-5	14.25107	13
CGACTAA	40	0.005274259	14.25107	13
ACCGACC	55	1.9565935E-4	13.819978	8
GCGTTAC	55	1.9575044E-4	13.819221	13
GGCCTAT	110	1.8735591E-10	13.807098	1
>>END_MODULE