FastQCFastQC Report
Thu 2 Feb 2017
SRR4062409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912888
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA61480.673467062772213No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC55120.6037980562785359No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA27170.2976268720806933No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC24930.2730893603596498No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG14860.16278010007799423No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA14690.1609178782063079No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC14520.15905565633462154No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13730.15040180175443207No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG13520.14810141003058425No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13430.14711552786322088No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12300.13473722953965875No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12210.13375134737229538No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11560.12663108727467115No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10680.11699135052711833No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10170.1114046849120593No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9690.10614664668612141No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA9400.10296991525795059No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC9370.10264128786882946No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG9260.10143632077538538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCTA250.00235259335.199698
CGCGTTA454.068708E-529.33307612
TCGTATA1053.6379788E-1227.23785844
CGGTGAT959.622454E-1025.47346343
CCGTTTA701.131848E-625.1426427
TTCGCGT551.5932953E-423.99979210
CTCGTAT703.2144566E-521.9998143
TACACGA602.8722006E-421.999815
CGCACGA1004.6213245E-821.99980742
CTCTTCG2450.021.5508338
CCGCACG1057.741619E-820.95219841
TGGTATA3550.020.45052544
TTGTCGG1401.8553692E-1020.42839422
ACTATAC550.004483010619.9998263
AGGTATA1653.6379788E-1219.9998257
ACGACGG2250.019.5553866
CGAAATT1504.656613E-1019.06650213
GTCCTAC2550.018.9854261
ACCGCTA708.121716E-418.8569818
CTAAGAC1051.7918574E-618.8569773
Produced by FastQC (version 0.11.5)