##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	912888
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.121137532753195	32.0	32.0	32.0	32.0	32.0
2	31.368317909754538	32.0	32.0	32.0	32.0	32.0
3	31.472903576342333	32.0	32.0	32.0	32.0	32.0
4	31.59157311740323	32.0	32.0	32.0	32.0	32.0
5	31.509675885760355	32.0	32.0	32.0	32.0	32.0
6	35.105643846780765	36.0	36.0	36.0	36.0	36.0
7	35.12541735678418	36.0	36.0	36.0	36.0	36.0
8	35.05907953659156	36.0	36.0	36.0	36.0	36.0
9	35.17634693412555	36.0	36.0	36.0	36.0	36.0
10	35.06878171254305	36.0	36.0	36.0	36.0	36.0
11	35.18739648237243	36.0	36.0	36.0	36.0	36.0
12	35.08917632831191	36.0	36.0	36.0	36.0	36.0
13	35.118803182865804	36.0	36.0	36.0	36.0	36.0
14	35.081577367650794	36.0	36.0	36.0	36.0	36.0
15	35.05533208893095	36.0	36.0	36.0	36.0	36.0
16	35.04186712937403	36.0	36.0	36.0	36.0	36.0
17	35.01990386553444	36.0	36.0	36.0	36.0	36.0
18	35.02588597944107	36.0	36.0	36.0	36.0	36.0
19	34.998472978065216	36.0	36.0	36.0	36.0	36.0
20	34.96892608950934	36.0	36.0	36.0	36.0	36.0
21	34.91959254585448	36.0	36.0	36.0	32.0	36.0
22	34.89532012689399	36.0	36.0	36.0	32.0	36.0
23	34.82794055787785	36.0	36.0	36.0	32.0	36.0
24	34.792593395903985	36.0	36.0	36.0	32.0	36.0
25	34.765866130346765	36.0	36.0	36.0	32.0	36.0
26	34.69541170439309	36.0	36.0	36.0	32.0	36.0
27	34.660419459999474	36.0	36.0	36.0	32.0	36.0
28	34.609212740226624	36.0	36.0	36.0	32.0	36.0
29	34.563345120102355	36.0	36.0	36.0	32.0	36.0
30	34.542303108376935	36.0	36.0	36.0	32.0	36.0
31	34.51897604087248	36.0	36.0	36.0	32.0	36.0
32	34.46668046901701	36.0	36.0	36.0	32.0	36.0
33	34.39988037963036	36.0	36.0	36.0	32.0	36.0
34	34.37554223519205	36.0	36.0	36.0	32.0	36.0
35	34.26662964131416	36.0	36.0	36.0	32.0	36.0
36	34.239812550937245	36.0	36.0	36.0	32.0	36.0
37	34.1549116649578	36.0	36.0	36.0	32.0	36.0
38	34.06388954614367	36.0	36.0	36.0	32.0	36.0
39	33.9099845764212	36.0	36.0	36.0	32.0	36.0
40	33.78869039794586	36.0	36.0	36.0	32.0	36.0
41	33.798104477219546	36.0	36.0	36.0	32.0	36.0
42	33.59210658919824	36.0	36.0	36.0	27.0	36.0
43	33.649071956253124	36.0	36.0	36.0	27.0	36.0
44	33.62234249984664	36.0	36.0	36.0	27.0	36.0
45	33.39758765587892	36.0	36.0	36.0	21.0	36.0
46	33.53472605620843	36.0	36.0	36.0	21.0	36.0
47	33.39260456923522	36.0	36.0	36.0	21.0	36.0
48	33.40999772151677	36.0	36.0	36.0	21.0	36.0
49	33.46632116973824	36.0	36.0	36.0	21.0	36.0
50	32.82325433130899	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	12.0
21	22.0
22	111.0
23	311.0
24	927.0
25	2326.0
26	4973.0
27	9426.0
28	15938.0
29	22458.0
30	30381.0
31	41426.0
32	59120.0
33	79071.0
34	157880.0
35	488502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.16639368060083	17.462379963735764	11.973880985379267	25.397345370284143
2	15.067180079860218	21.011015013501744	36.00556501925299	27.916239887385043
3	19.624792278511656	24.8267846521069	28.09982900290399	27.44859406647745
4	12.413269564874476	15.870875443374091	35.034823625293846	36.68103136645758
5	13.781427732646284	37.099293670198314	33.48307788030952	15.636200716845877
6	34.6471563168555	34.128579456513044	16.891430774809038	14.332833451822413
7	29.34883578270281	30.925370910779854	20.802223273829867	18.92357003268747
8	29.358365976987322	31.932175688583925	20.137410065637845	18.572048268790915
9	27.54620501091043	14.539461576885666	18.058951371909806	39.8553820402941
10	16.357756920892815	27.086345751066943	31.432990684508944	25.1229066435313
11	37.74186975839314	21.260768024116867	20.314868855763248	20.68249336172674
12	23.30581626661759	24.565883218971003	28.87878907379657	23.24951144061484
13	31.034694288894148	19.179351683886743	24.948405499907985	24.837548527311128
14	24.49763826449685	19.374446810561647	24.299475948856813	31.828438976084687
15	26.52099709931558	25.559323816284145	24.46291330371305	23.456765780687224
16	26.684105826782694	25.26498321809466	24.63248503649955	23.418425918623097
17	23.92166399383057	25.48768304545574	26.635468973192765	23.955183987520922
18	26.100682668629666	23.009394361630342	28.26250317673143	22.627419793008563
19	24.92003400198053	27.577643697803015	24.781791413623576	22.72053088659288
20	24.16320512483459	23.193644784464247	28.620049776095208	24.023100314605955
21	26.147238215421826	25.021141695366794	23.468705909158626	25.362914180052755
22	23.84651786418487	25.527337417076357	25.12630245988555	25.49984225885322
23	22.89656562469876	24.754624882789564	25.437950767235414	26.910858725276267
24	23.086840882999887	27.427680065900745	24.83831532455241	24.647163726546957
25	25.367624505963494	24.308677515752205	24.233969555958673	26.08972842232563
26	23.17305080141266	24.830537809676542	25.874148855062177	26.122262533848623
27	26.365008631946086	25.316796803112755	25.247346881545162	23.070847683395993
28	24.233750471032593	23.61932679583914	26.07307796794349	26.073844765184777
29	23.270434051055553	25.721556204046937	27.048553601318016	23.959456143579498
30	25.2059398305159	24.845654669576113	27.01492406516462	22.933481434743364
31	23.46969179132599	25.1291505639246	25.492283828903435	25.908873815845972
32	23.71671004548203	25.413303713051327	25.680477780406797	25.189508461059845
33	23.1726126315605	24.675425682011376	25.87009578392968	26.281865902498446
34	23.05879801246155	26.090057049714748	27.118441692737772	23.732703245085926
35	25.767783123449973	24.890128909570507	26.02498882666877	23.317099140310752
36	23.884200471470763	26.256232966146996	25.5098106229899	24.349755939392345
37	25.651449027701094	25.510796505157256	25.811271481277004	23.02648298586464
38	24.25971203477316	25.338595753257792	24.789021216184242	25.612670995784807
39	24.28041556028779	25.133970432298376	25.962111452883597	24.623502554530237
40	25.25786295799704	25.596568253717873	24.696567377378166	24.449001410906924
41	22.836317270026555	25.71673633567316	26.472798415577813	24.97414797872247
42	26.640179299103504	25.867795392205835	25.204625320959416	22.287399987731245
43	25.079418285704268	23.643973850023222	24.766236383871846	26.510371480400664
44	24.137791273409224	24.746080570672415	27.105406139635967	24.01072201628239
45	25.009639736747552	25.108885208262134	25.602045376869892	24.279429678120426
46	23.729797586993335	24.820733366488255	27.178530506547364	24.270938539971038
47	25.019717643347267	24.111610624742575	27.104201172542524	23.764470559367634
48	24.204940803252974	28.298323562145626	23.94543470831033	23.551300926291066
49	22.97357397621614	25.33728124370131	27.738890203398448	23.950254576684106
50	22.795677016238574	27.99554819430204	23.71550507838859	25.4932697110708
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	21.5
2	23.0
3	98.0
4	173.0
5	187.0
6	201.0
7	212.0
8	223.0
9	287.5
10	352.0
11	518.5
12	685.0
13	1216.0
14	1747.0
15	2492.0
16	3237.0
17	3983.5
18	4730.0
19	5106.5
20	5483.0
21	5986.5
22	6490.0
23	6782.0
24	7074.0
25	6587.0
26	6100.0
27	7081.0
28	8062.0
29	9071.5
30	10081.0
31	10815.0
32	11549.0
33	13544.5
34	15540.0
35	17699.5
36	19859.0
37	24110.5
38	28362.0
39	30630.5
40	32899.0
41	37970.0
42	43041.0
43	47655.5
44	52270.0
45	63528.5
46	74787.0
47	84750.0
48	94713.0
49	90885.5
50	87058.0
51	77792.5
52	68527.0
53	62895.5
54	57264.0
55	57793.0
56	58322.0
57	55756.0
58	53190.0
59	49349.5
60	45509.0
61	39725.5
62	33942.0
63	29121.0
64	24300.0
65	20904.0
66	17508.0
67	14881.5
68	12255.0
69	11512.0
70	10769.0
71	8198.0
72	5627.0
73	5081.0
74	4535.0
75	3190.0
76	1845.0
77	1713.5
78	1582.0
79	1373.0
80	1164.0
81	953.5
82	743.0
83	606.0
84	469.0
85	405.5
86	342.0
87	250.0
88	158.0
89	103.0
90	48.0
91	33.0
92	18.0
93	14.0
94	10.0
95	6.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015664572214773333
2	0.004710325910736037
3	7.667972412826108E-4
4	2.1908492608074595E-4
5	0.0
6	5.477123152018649E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.1908492608074595E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	912888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.50738528424526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5741948976505	37.328545504020596
2	12.580575511801179	11.953404958733591
3	3.7510154685829735	5.346028112194053
4	1.531002320491278	2.9093566844261076
5	0.8224735468912959	1.9536783839132277
6	0.47561365978197234	1.3557096829027262
7	0.3433684882801689	1.1418777347036386
8	0.24286118114604133	0.9230159762633493
9	0.18264065165392845	0.7809101826019924
>10	1.179550333786115	11.141395147462225
>50	0.16020471457987745	5.3841568935970585
>100	0.14376630021787257	13.326892690069492
>500	0.009260309190432421	3.0414773248052365
>1k	0.0030096004868905364	2.131145806131159
>5k	4.63015459521621E-4	1.2824049181756365
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6148	0.673467062772213	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5512	0.6037980562785359	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2717	0.2976268720806933	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2493	0.2730893603596498	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1486	0.16278010007799423	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1469	0.1609178782063079	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1452	0.15905565633462154	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1373	0.15040180175443207	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1352	0.14810141003058425	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1343	0.14711552786322088	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1230	0.13473722953965875	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1221	0.13375134737229538	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1156	0.12663108727467115	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1068	0.11699135052711833	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1017	0.1114046849120593	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	969	0.10614664668612141	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	940	0.10296991525795059	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	937	0.10264128786882946	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	926	0.10143632077538538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0954246304037297E-4
2	0.0	0.0	0.0	0.0	1.0954246304037297E-4
3	0.0	0.0	0.0	0.0	1.0954246304037297E-4
4	0.0	0.0	0.0	0.0	1.0954246304037297E-4
5	0.0	0.0	0.0	0.0	1.0954246304037297E-4
6	0.0	0.0	0.0	0.0	1.0954246304037297E-4
7	0.0	0.0	0.0	0.0	1.0954246304037297E-4
8	0.0	0.0	0.0	0.0	1.0954246304037297E-4
9	0.0	0.0	0.0	1.0954246304037297E-4	1.0954246304037297E-4
10	0.0	0.0	0.0	2.1908492608074595E-4	1.0954246304037297E-4
11	0.0	0.0	0.0	2.1908492608074595E-4	1.0954246304037297E-4
12	0.0	0.0	0.0	2.1908492608074595E-4	3.286273891211189E-4
13	0.0	0.0	0.0	2.1908492608074595E-4	3.286273891211189E-4
14	0.0	0.0	0.0	2.1908492608074595E-4	3.286273891211189E-4
15	0.0	0.0	0.0	3.286273891211189E-4	4.381698521614919E-4
16	0.0	0.0	0.0	4.381698521614919E-4	4.381698521614919E-4
17	0.0	0.0	0.0	4.381698521614919E-4	4.381698521614919E-4
18	0.0	0.0	0.0	6.572547782422378E-4	4.381698521614919E-4
19	0.0	0.0	0.0	0.0010954246304037297	4.381698521614919E-4
20	0.0	0.0	0.0	0.0018622218716863406	4.381698521614919E-4
21	0.0	0.0	0.0	0.0026290191129689513	4.381698521614919E-4
22	0.0	0.0	0.0	0.003724443743372681	4.381698521614919E-4
23	0.0	0.0	0.0	0.00624392039330126	4.381698521614919E-4
24	0.0	0.0	0.0	0.009420651821472075	4.381698521614919E-4
25	0.0	0.0	0.0	0.012378298323562145	4.381698521614919E-4
26	0.0	0.0	0.0	0.016759996845177063	5.477123152018648E-4
27	0.0	0.0	0.0	0.022675289849357204	7.667972412826108E-4
28	0.0	0.0	0.0	0.035929927877242335	7.667972412826108E-4
29	0.0	0.0	0.0	0.05652391092883245	7.667972412826108E-4
30	0.0	0.0	0.0	0.09267292373215553	8.763397043229838E-4
31	0.0	0.0	0.0	0.15368807564564327	8.763397043229838E-4
32	0.0	0.0	0.0	0.23201093671950995	8.763397043229838E-4
33	0.0	0.0	0.0	0.30058451858278346	8.763397043229838E-4
34	0.0	0.0	0.0	0.38569901236515325	8.763397043229838E-4
35	0.0	0.0	0.0	0.4810009552102777	8.763397043229838E-4
36	0.0	0.0	0.0	0.620886680512834	8.763397043229838E-4
37	0.0	0.0	0.0	0.8177344865963843	8.763397043229838E-4
38	0.0	0.0	0.0	1.0468973192768445	8.763397043229838E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCTA	25	0.002352593	35.19969	8
CGCGTTA	45	4.068708E-5	29.333076	12
TCGTATA	105	3.6379788E-12	27.237858	44
CGGTGAT	95	9.622454E-10	25.473463	43
CCGTTTA	70	1.131848E-6	25.14264	27
TTCGCGT	55	1.5932953E-4	23.999792	10
CTCGTAT	70	3.2144566E-5	21.99981	43
TACACGA	60	2.8722006E-4	21.99981	5
CGCACGA	100	4.6213245E-8	21.999807	42
CTCTTCG	245	0.0	21.55083	38
CCGCACG	105	7.741619E-8	20.952198	41
TGGTATA	355	0.0	20.450525	44
TTGTCGG	140	1.8553692E-10	20.428394	22
ACTATAC	55	0.0044830106	19.999826	3
AGGTATA	165	3.6379788E-12	19.999825	7
ACGACGG	225	0.0	19.555386	6
CGAAATT	150	4.656613E-10	19.066502	13
GTCCTAC	255	0.0	18.985426	1
ACCGCTA	70	8.121716E-4	18.85698	18
CTAAGAC	105	1.7918574E-6	18.856977	3
>>END_MODULE