FastQCFastQC Report
Thu 2 Feb 2017
SRR4062408_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062408_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063670
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38000.3572536594996568No Hit
GTACATGGAAGCAGTGGTATCAACG32400.30460575178391797No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28220.2653078492389557No Hit
TCCATGTACTCTGCGTTGATACCAC26850.25242791467278386No Hit
GGGTAGGCACACGCTGAGCCAGTCA26250.24678706741752612No Hit
GTATTAGAGGCACTGCCTGCCCAGT26140.24575291208739553No Hit
TATCAACGCAGAGTACTTTTTTTTT25310.23794974005095565No Hit
CCATAGGGTCTTCTCGTCTTATTAT22600.21247191328137485No Hit
GAATAATTGCAATCCCCGATCCCCA22440.21096768734663948No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC22060.20739515075164291No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21430.20147226113362227No Hit
GTACATGGGGAATAATTGCAATCCC20750.19507930091099684No Hit
CTCTAATACTTGTAATGCTAGAGGT20340.19122472195323736No Hit
CATCTAAGGGCATCACAGACCTGTT19960.1876521853582408No Hit
GTGCATGGCCGTTCTTAGTTGGTGG19470.18304549343311366No Hit
CCATTGGGATGTCCTGATCCAACAT19020.17881485799167035No Hit
GCGCAAGACGGACCAGAGCGAAAGC16610.15615745484971844No Hit
GATTAAGAGGGACGGCCGGGGGCAT16540.1554993560032717No Hit
GATATATTTTGATCAACGGACCAAG16290.15314900298024764No Hit
AGTCAGTGTAGCGCGCGTGCAGCCC15980.1502345652316978No Hit
CTTCTACACCATTGGGATGTCCTGA15870.14920040990156722No Hit
GGATTGACAGATTGATAGCTCTTTC15680.14741414160406893No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15680.14741414160406893No Hit
GTTATTGCTCAATCTCGGGTGGCTG15550.14619195803209642No Hit
GAATAGGACCGCGGTTCTATTTTGT15550.14619195803209642No Hit
TCGTAGTTCCGACCATAAACGATGC15390.14468773209736102No Hit
GAATAACGCCGCCGCATCGCCAGTC15230.14318350616262562No Hit
GAACTACGACGGTATCTGATCGTCT14940.1404570966559177No Hit
TCACAGACCTGTTATTGCTCAATCT14840.13951695544670809No Hit
CCCCGATCCCCATCACGAATGGGGT14760.1387648424793404No Hit
GTCAGGATACCGCGGCCGTTAAACT14640.13763667302828886No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14580.1370725883027631No Hit
ATCTATAACTTTATAGATGCAACAC14560.13688456006092115No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA14200.1335000517077665No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14160.13312399522408266No Hit
GTATCTGATCGTCTTCGAACCTCCG14080.13237188225671495No Hit
GTATCCTGACCGTGCAAAGGTAGCA13900.13067962808013764No Hit
CTGTTAGTATGAGTAACAAGAATTC13680.12861131741987647No Hit
GCCATGCACCACCACCCACGGAATC13620.1280472326943507No Hit
ACACTGACTGGCTCAGCGTGTGCCT13470.12663702088053624No Hit
ATCCTGACCGTGCAAAGGTAGCATA13200.12409863961567029No Hit
GAGTACATGGAAGCAGTGGTATCAA13070.12287645604369776No Hit
GTATCAACGCAGAGTACATGGGGAA12950.12174828659264622No Hit
GTTATAGATTAACCCAATTTTAAGT12880.12109018774619949No Hit
ATCAGATACCGTCGTAGTTCCGACC12850.1208081453834366No Hit
GTTATATAATTTAAGCTCCATAGGG12790.12024406065791082No Hit
CCTCTAGCATTACAAGTATTAGAGG12780.12015004653698985No Hit
GGTTAATTCCGATAACGAACGAGAC12730.11967997593238505No Hit
GCCTAAAGGAAAGATCCAAAAAGAT12430.11685955230475618No Hit
ATCGTAAATAGATAGAAACCGACCT12390.11648349582107233No Hit
GCCCCGGACATCTAAGGGCATCACA12350.11610743933738847No Hit
CTCTTAATCATGGCCTCAGTTCCGA12180.11450919928173212No Hit
GTTCATGCTAGTCCCTAATTAAGGA12110.11385110043528539No Hit
CGCTGAGCCAGTCAGTGTAGCGCGC12100.1137570863143644No Hit
GGAGTGGAGCCTGCGGCTTAATTTG12020.11300497334699672No Hit
GGGTTCAACGGGTTACCCGCGCCTG11990.11272293098423385No Hit
GTACATGGGAGAAATCGTAAATAGA11790.11084264856581459No Hit
GGTATCAACGCAGAGTACTTTTTTT11750.11046659208213074No Hit
GGTCAGGATACCGCGGCCGTTAAAC11710.11009053559844688No Hit
GTCCTATTCCATTATTCCTAGCTGC11690.10990250735660495No Hit
TCTAAGGGCATCACAGACCTGTTAT11670.10971447911476305No Hit
CATGTACTCTGCGTTGATACCACTG11640.10943243675200015No Hit
TGCCTACCCTACGCCGGCAGGCGCG11490.10802222493818572No Hit
CTATAGAACTAGTACCGCAAGGGAA11470.1078341966963438No Hit
ATCTCGGGTGGCTGAACGCCACTTG11430.10745814021265994No Hit
GAATAATGGAATAGGACCGCGGTTC11350.10670602724529225No Hit
GTATCAACGCAGAGTACATGGGAGA11290.10614194251976647No Hit
CCTTAGATGTCCGGGGCTGCACGCG11100.10435567422226819No Hit
CCTATAACTTCTCTGTTAACCCAAC11080.10416764598042627No Hit
CACCAGGAGTGGAGCCTGCGGCTTA11000.10341553301305856No Hit
TGCTAGAGGTGATGTTTTTGGTAAA10870.10219334944108606No Hit
GATTAAAGATAAGAGACAGTTGGAC10810.10162926471556027No Hit
ACCTATAACTTCTCTGTTAACCCAA10790.10144123647371835No Hit
CACTATAAATAATCCACCTATAACT10710.10068912350635066No Hit
AAATATATCTGGGTCAATAAGATAT10710.10068912350635066No Hit
TCCCAATGGTGTAGAAGCTATTAAT10690.10050109526450873No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT10680.10040708114358776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA307.755728E-418.9871541
GACTGGT350.002168477516.2869667
CCAATTT1500.014.56847214
AACTTGC851.7127604E-814.5305287
CGCTAAG400.005294766714.2430462
CCCAATT1650.013.81989513
CTGTGCA551.9571734E-413.8198959
GCGTTCC707.248078E-613.5731119
TACGCCT707.262195E-613.5705545
TGGACAG500.001499729713.2991445
GAGCGTT655.4453863E-513.1548587
CTAAGCC655.455019E-513.1523814
AGGCCCG2400.013.06411910
TTAGGCA1600.013.0610434
AACCTTG2550.013.0396036
TTGCTAA1102.737579E-912.95615210
TGCTAAA1102.739398E-912.95554111
TCGGCAA1251.0186341E-1012.92160113
GAACGCG751.4749432E-512.66883319
TAGTACG604.1070065E-412.6604852
Produced by FastQC (version 0.11.5)