FastQCFastQC Report
Thu 2 Feb 2017
SRR4062407_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062407_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850975
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG49040.26494144977646916No Hit
GTATCAACGCAGAGTACTTTTTTTT49020.2648333986142439No Hit
TCCATGTACTCTGCGTTGATACCAC38730.20924107564931996No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35600.19233106876105835No Hit
TATCAACGCAGAGTACTTTTTTTTT34510.18644228041977878No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24920.13463174813274087No Hit
GAGTACATGGAAGCAGTGGTATCAA20540.11096854360539715No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19130.10335093666851254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA601.4661109E-615.8344389
ATTCCGA1006.5847416E-1014.2509946
CGCGCCA1205.0931703E-1113.45927210
GCGTTAT957.4616764E-812.9903631
CGAACGA1051.9877916E-812.66960816
AACCGCG1207.403287E-1012.6675517
GCGTAAC905.402235E-712.66755111
TCCAACG2350.012.53516318
CGTTATT1001.4534089E-712.3431812
TACGCTA1850.012.3251849
AAGACGG2550.012.292985
CCAACGA1650.012.09404319
GCTCTAA951.0494732E-611.9911041
AGGACGT6500.011.6912265
CTAGGAC2450.011.6287453
CGCGTAA907.4567906E-611.61192110
TAGTACG907.50861E-611.60472
TAGGACG6550.011.6010384
ATACGCT2050.011.5861748
TTAGGAC4850.011.5528183
Produced by FastQC (version 0.11.5)