##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062407_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1850975
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15239319817934	32.0	32.0	32.0	32.0	32.0
2	30.81949459068869	32.0	32.0	32.0	32.0	32.0
3	30.8229327786707	32.0	32.0	32.0	32.0	32.0
4	30.883341482191817	32.0	32.0	32.0	32.0	32.0
5	30.687539810100084	32.0	32.0	32.0	32.0	32.0
6	34.32866030065236	36.0	36.0	36.0	32.0	36.0
7	34.206416078012936	36.0	36.0	36.0	32.0	36.0
8	34.19792973973176	36.0	36.0	36.0	32.0	36.0
9	34.354718999446234	36.0	36.0	36.0	32.0	36.0
10	34.031207336673916	36.0	36.0	36.0	32.0	36.0
11	34.35934629046854	36.0	36.0	36.0	32.0	36.0
12	34.141912775699296	36.0	36.0	36.0	32.0	36.0
13	34.25379543213712	36.0	36.0	36.0	32.0	36.0
14	34.17642485716987	36.0	36.0	36.0	32.0	36.0
15	34.10793554748173	36.0	36.0	36.0	32.0	36.0
16	34.118880589959346	36.0	36.0	36.0	32.0	36.0
17	34.04574345952808	36.0	36.0	36.0	32.0	36.0
18	34.05383162927646	36.0	36.0	36.0	32.0	36.0
19	34.03638838990262	36.0	36.0	36.0	32.0	36.0
20	34.02246437688245	36.0	36.0	36.0	32.0	36.0
21	33.98320884939019	36.0	36.0	36.0	32.0	36.0
22	33.93839517011305	36.0	36.0	36.0	32.0	36.0
23	33.90652980186118	36.0	36.0	36.0	32.0	36.0
24	33.884058941909	36.0	36.0	36.0	32.0	36.0
25	33.41057928929348	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	8.0
4	63.0
5	194.0
6	305.0
7	91.0
8	298.0
9	310.0
10	230.0
11	70.0
12	161.0
13	102.0
14	346.0
15	470.0
16	711.0
17	895.0
18	1336.0
19	1953.0
20	2680.0
21	3834.0
22	5621.0
23	8123.0
24	11227.0
25	15762.0
26	21277.0
27	27474.0
28	38010.0
29	50274.0
30	66021.0
31	89723.0
32	130667.0
33	189290.0
34	418334.0
35	765113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.69917106385002	17.37421426124618	11.337601023554063	25.589013651349735
2	16.202039106190526	20.255614943792484	38.04633290993776	25.496013040079223
3	19.28669026248411	24.546799842022203	28.23399299689393	27.932516898599758
4	12.399057864395369	15.65022540253927	36.26017121191081	35.690545521154554
5	14.028716129489025	37.09457666488859	34.11179369611402	14.764913509508359
6	33.43147180136068	35.67582847910959	17.39975039281512	13.492949326714617
7	29.410893347951795	30.74569285230814	21.314785621144004	18.528628178596065
8	27.593454671692662	33.49968285622978	20.064196604263515	18.84266586781404
9	27.38116418780544	14.42485786858854	18.721490572251646	39.472487371354376
10	15.381840113157844	27.497951312917685	32.49929005893187	24.620918514992603
11	36.913341241546135	21.43347445869041	22.525857205371743	19.127327094391713
12	24.102616815351407	23.967962000183938	29.891637768268204	22.037783416196447
13	29.78292502177241	20.1655784969735	25.385763819394487	24.665732661859607
14	23.094387266595913	20.052006417767217	25.835245572638588	31.018360742998286
15	24.724387898273147	27.858052330329937	23.172998181517247	24.244561589879673
16	24.98369253036759	26.136303402230364	25.06931350691188	23.810690560490166
17	23.3284576295208	26.088946252938676	26.323282964283667	24.25931315325686
18	23.95953700938705	25.437272518750298	27.617305669882242	22.985884801980415
19	25.075318843911877	25.68327946009548	25.788988220019206	23.452413475973437
20	24.85996818282381	25.092187049511356	26.40414521225064	23.643699555414194
21	25.80599266010577	24.940006318541023	24.81909930496049	24.43490171639272
22	24.649353249444694	25.589786184077028	25.708233116983255	24.052627449495017
23	23.868145328397727	25.133227797080405	25.909438641633724	25.089188232888144
24	23.995901428711313	25.86741641235436	25.905123701005543	24.23155845792878
25	24.346867365332304	25.3713870236887	26.02527703903107	24.256468571947917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	323.0
1	323.0
2	829.0
3	1335.0
4	1335.0
5	1335.0
6	3511.5
7	5688.0
8	5688.0
9	5688.0
10	5835.0
11	5982.0
12	5982.0
13	5982.0
14	6424.0
15	6866.0
16	6866.0
17	6866.0
18	11211.0
19	15556.0
20	15556.0
21	15556.0
22	24323.5
23	33091.0
24	33091.0
25	33091.0
26	48853.0
27	64615.0
28	64615.0
29	64615.0
30	82015.5
31	99416.0
32	99416.0
33	99416.0
34	113437.0
35	127458.0
36	127458.0
37	127458.0
38	141661.0
39	155864.0
40	155864.0
41	155864.0
42	175962.5
43	196061.0
44	196061.0
45	196061.0
46	222654.0
47	249247.0
48	249247.0
49	249247.0
50	243284.5
51	237322.0
52	237322.0
53	237322.0
54	217664.5
55	198007.0
56	198007.0
57	198007.0
58	179702.0
59	161397.0
60	161397.0
61	161397.0
62	141864.5
63	122332.0
64	122332.0
65	122332.0
66	101409.0
67	80486.0
68	80486.0
69	80486.0
70	61936.0
71	43386.0
72	43386.0
73	43386.0
74	33424.0
75	23462.0
76	23462.0
77	23462.0
78	18340.0
79	13218.0
80	13218.0
81	13218.0
82	9368.5
83	5519.0
84	5519.0
85	5519.0
86	4028.0
87	2537.0
88	2537.0
89	2537.0
90	1683.5
91	830.0
92	830.0
93	830.0
94	508.0
95	186.0
96	186.0
97	186.0
98	488.5
99	791.0
100	791.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009130323208039007
2	0.0035656883534353513
3	0.004430097651237861
4	0.015451316198219857
5	0.03544078120990289
6	0.046894204405786136
7	0.06801820662083496
8	0.08946636232255972
9	0.09757019948945826
10	0.12047704588122475
11	0.1223139156390551
12	0.1374951039317117
13	0.12452896446467401
14	0.12733829468253216
15	0.11782979240670458
16	0.11502046218884643
17	0.12712219235808153
18	0.13349721092937505
19	0.1350639527816421
20	0.12361052958575885
21	0.13220059698267128
22	0.15613392941557827
23	0.1424654573940761
24	0.1364686178905712
25	0.13500992720052946
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1850975.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.35566284373012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.92315037784284	39.750068632161614
2	14.386597572134729	15.064397039102245
3	4.477894863354038	7.03329461146305
4	1.8676904635880878	3.911366888322717
5	0.9199799339561474	2.408307962260258
6	0.5494332141799445	1.7259564070051279
7	0.34466270029910734	1.2631530892168765
8	0.23852264100959203	0.9990408778635419
9	0.19069263245242607	0.8985455254316312
>10	0.9156188885418288	8.855359213810576
>50	0.08981253530295463	3.279814498629326
>100	0.08549145284000116	9.249565253620862
>500	0.007244462523598318	2.6002403455324887
>1k	0.003208261974736398	2.96088965557988
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4904	0.26494144977646916	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4902	0.2648333986142439	No Hit
TCCATGTACTCTGCGTTGATACCAC	3873	0.20924107564931996	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3560	0.19233106876105835	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3451	0.18644228041977878	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2492	0.13463174813274087	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2054	0.11096854360539715	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1913	0.10335093666851254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.402558111265684E-5
7	0.0	0.0	0.0	5.402558111265684E-5	5.402558111265684E-5
8	0.0	0.0	0.0	5.402558111265684E-5	5.402558111265684E-5
9	0.0	0.0	0.0	1.6207674333797053E-4	5.402558111265684E-5
10	0.0	0.0	0.0	2.1610232445062736E-4	5.402558111265684E-5
11	0.0	0.0	0.0	2.1610232445062736E-4	5.402558111265684E-5
12	0.0	0.0	0.0	2.1610232445062736E-4	5.402558111265684E-5
13	0.0	0.0	0.0	2.1610232445062736E-4	5.402558111265684E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	60	1.4661109E-6	15.834438	9
ATTCCGA	100	6.5847416E-10	14.250994	6
CGCGCCA	120	5.0931703E-11	13.459272	10
GCGTTAT	95	7.4616764E-8	12.990363	1
CGAACGA	105	1.9877916E-8	12.669608	16
AACCGCG	120	7.403287E-10	12.667551	7
GCGTAAC	90	5.402235E-7	12.667551	11
TCCAACG	235	0.0	12.535163	18
CGTTATT	100	1.4534089E-7	12.343181	2
TACGCTA	185	0.0	12.325184	9
AAGACGG	255	0.0	12.29298	5
CCAACGA	165	0.0	12.094043	19
GCTCTAA	95	1.0494732E-6	11.991104	1
AGGACGT	650	0.0	11.691226	5
CTAGGAC	245	0.0	11.628745	3
CGCGTAA	90	7.4567906E-6	11.611921	10
TAGTACG	90	7.50861E-6	11.6047	2
TAGGACG	655	0.0	11.601038	4
ATACGCT	205	0.0	11.586174	8
TTAGGAC	485	0.0	11.552818	3
>>END_MODULE