FastQCFastQC Report
Thu 2 Feb 2017
SRR4062407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850975
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21710.117289536595578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCA1700.020.7067974
ACCGTTC755.4750617E-520.5331338
TAACGGC1950.020.30749336
TAGGACG7600.018.8166224
ATACCGT3250.017.6003026
GACCGTT1154.275762E-617.217227
TTAACGG2300.017.2172235
TAATACG902.215423E-417.1118684
GATATAC7250.016.6926481
AGGACGT8050.016.6710935
TAGTACG1854.2200554E-1016.6498362
GTCCTAA6550.016.4609661
GATTTCG3500.016.34269741
TACCGTC3100.016.322427
ATACACT8900.016.3153294
CGTCGTA3250.016.24599510
GGACGTG7900.016.1521766
ATATACT3750.015.8407014
ACCGTCG3200.015.8123448
GTATAGA2650.015.7764141
Produced by FastQC (version 0.11.5)