FastQCFastQC Report
Thu 2 Feb 2017
SRR4062406_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062406_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018424
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC26580.13168690027466975No Hit
GTCTTGCGCCGGTCCAAGAATTTCA25770.1276738683249902No Hit
GTATCAACGCAGAGTACTTTTTTTT22850.11320713586441698No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21930.1086491242672501No Hit
GAATAGGACCGCGGTTCTATTTTGT20680.10245617372762114No Hit
GGGTAGGCACACGCTGAGCCAGTCA20590.10201028128876788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAA655.439705E-513.1575419
CGTCTTA2700.013.02088115
CGCAAGA4200.012.2068112
CGCGCTA1251.4060788E-912.16311416
GACCGTA550.003067098612.0919047
GGTCCAA6050.012.09100411
CGGTCCA5350.012.0754610
AAGACGG4250.012.0694865
GGTATCA7250.012.0474971
CGCGCCA951.0395488E-612.000689510
GCGGGTA802.8583965E-511.8786318
CGCATCG3100.011.64640813
AGAATTT5800.011.63257517
ATAGGGT3350.011.6209023
CAAGACG4650.011.4383964
GCGCCGA2000.011.40320119
TAACGAA1501.7644197E-1011.40122113
CGGCTAA1251.8297214E-811.4012219
TTCTCGT3250.011.40008911
CGCCGGT5200.011.3278557
Produced by FastQC (version 0.11.5)