##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062406_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2018424
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.267895149879312	32.0	32.0	32.0	32.0	32.0
2	30.932103462899768	32.0	32.0	32.0	32.0	32.0
3	30.917102154948612	32.0	32.0	32.0	32.0	32.0
4	30.99701797045616	32.0	32.0	32.0	32.0	32.0
5	30.786488864579493	32.0	32.0	32.0	32.0	32.0
6	34.45200661506205	36.0	36.0	36.0	32.0	36.0
7	34.35815269735199	36.0	36.0	36.0	32.0	36.0
8	34.360656135678134	36.0	36.0	36.0	32.0	36.0
9	34.530275601162096	36.0	36.0	36.0	32.0	36.0
10	34.20478947931654	36.0	36.0	36.0	32.0	36.0
11	34.4778381549169	36.0	36.0	36.0	32.0	36.0
12	34.27777067652782	36.0	36.0	36.0	32.0	36.0
13	34.403345877773944	36.0	36.0	36.0	32.0	36.0
14	34.305652826165364	36.0	36.0	36.0	32.0	36.0
15	34.242249893976684	36.0	36.0	36.0	32.0	36.0
16	34.239371410565866	36.0	36.0	36.0	32.0	36.0
17	34.17254303357471	36.0	36.0	36.0	32.0	36.0
18	34.185397121714765	36.0	36.0	36.0	32.0	36.0
19	34.16978692286656	36.0	36.0	36.0	32.0	36.0
20	34.18072268264745	36.0	36.0	36.0	32.0	36.0
21	34.16469780383111	36.0	36.0	36.0	32.0	36.0
22	34.121542847290755	36.0	36.0	36.0	32.0	36.0
23	34.08300485923671	36.0	36.0	36.0	32.0	36.0
24	34.05245627281483	36.0	36.0	36.0	32.0	36.0
25	33.60493880373995	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	81.0
5	197.0
6	345.0
7	74.0
8	284.0
9	388.0
10	248.0
11	80.0
12	151.0
13	133.0
14	295.0
15	363.0
16	565.0
17	702.0
18	901.0
19	1350.0
20	2021.0
21	3067.0
22	4443.0
23	7074.0
24	10030.0
25	14848.0
26	20868.0
27	27836.0
28	38304.0
29	51826.0
30	67553.0
31	94233.0
32	136509.0
33	200930.0
34	457810.0
35	874906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.66319616657806	17.32898170641977	10.985512689464977	26.022309437537196
2	17.58396710184061	19.104020610895038	36.29132707409518	27.02068521316917
3	19.366681827775746	22.2571026823272	28.270414730652494	30.10580075924456
4	12.974979126561173	14.43109039871365	35.29883083966969	37.295099635055486
5	15.011842768476974	35.76699458340168	33.59262363290243	15.628539015218912
6	35.656854799735314	34.53142697110964	15.988262439558751	13.823455789596299
7	31.35624479435212	30.06459961131163	19.882748978701464	18.696406615634793
8	28.52739425441422	32.738423759886025	18.85640696833149	19.877775017368265
9	26.704925569209454	14.612903849711149	18.75322660712931	39.92894397395009
10	16.17159849899677	26.141464346212896	31.136392383871904	26.55054477091843
11	37.749685637541944	21.18557429742781	21.71930842739405	19.345431637636192
12	24.586423642289816	23.55497566614245	28.154694871782148	23.703905819785582
13	28.860273874753346	18.855353137317348	25.514866877186925	26.769506110742388
14	23.066280733867586	19.662730449750608	24.640073019676123	32.630915796705686
15	25.195080770283834	27.019207494910937	21.785904186722007	25.99980754808322
16	26.231383241234308	25.96279897050136	23.04046322478693	24.7653545634774
17	24.26824319833008	26.17230401081005	24.760628244825394	24.798824546034478
18	25.093339762689297	24.900806348393704	25.684731370990704	24.321122517926295
19	25.484875168983866	25.126989048605342	25.01705341750487	24.371082364905927
20	25.255636807731953	24.746893021936103	24.73478952313207	25.262680647199875
21	26.343372286615192	24.34441209913144	24.555301555551587	24.75691405870178
22	25.62274666484068	24.328477591440876	24.843128673686802	25.205647070031645
23	24.39389594448838	24.23126269486966	25.456767239967892	25.918074120674067
24	24.64114310321049	25.110606407878926	24.953341409907992	25.294909079002593
25	24.813655614243185	24.668995320368477	25.003609065973727	25.513739999414614
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	121.0
1	121.0
2	357.5
3	594.0
4	594.0
5	594.0
6	1708.0
7	2822.0
8	2822.0
9	2822.0
10	3243.5
11	3665.0
12	3665.0
13	3665.0
14	4705.5
15	5746.0
16	5746.0
17	5746.0
18	9455.0
19	13164.0
20	13164.0
21	13164.0
22	20834.0
23	28504.0
24	28504.0
25	28504.0
26	42369.5
27	56235.0
28	56235.0
29	56235.0
30	69236.0
31	82237.0
32	82237.0
33	82237.0
34	102452.5
35	122668.0
36	122668.0
37	122668.0
38	139880.5
39	157093.0
40	157093.0
41	157093.0
42	178400.5
43	199708.0
44	199708.0
45	199708.0
46	226779.5
47	253851.0
48	253851.0
49	253851.0
50	259673.5
51	265496.0
52	265496.0
53	265496.0
54	252921.5
55	240347.0
56	240347.0
57	240347.0
58	223298.5
59	206250.0
60	206250.0
61	206250.0
62	182412.5
63	158575.0
64	158575.0
65	158575.0
66	131122.0
67	103669.0
68	103669.0
69	103669.0
70	78832.0
71	53995.0
72	53995.0
73	53995.0
74	42399.5
75	30804.0
76	30804.0
77	30804.0
78	25152.0
79	19500.0
80	19500.0
81	19500.0
82	13509.5
83	7519.0
84	7519.0
85	7519.0
86	5528.5
87	3538.0
88	3538.0
89	3538.0
90	2434.0
91	1330.0
92	1330.0
93	1330.0
94	763.0
95	196.0
96	196.0
97	196.0
98	496.5
99	797.0
100	797.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008670130755480513
2	0.003666226719460331
3	0.005053447640337214
4	0.015308973733962734
5	0.03651363638165222
6	0.04800775258320353
7	0.0685683483747716
8	0.08992164183541218
9	0.09844314177794161
10	0.12083685092923985
11	0.12083685092923985
12	0.13446134211642352
13	0.12217452824579969
14	0.1252957753177727
15	0.11573385968458559
16	0.11380165911622138
17	0.12494897008755346
18	0.13228142352647412
19	0.13371818805166805
20	0.12276905149760407
21	0.1325786851523763
22	0.15249521408782296
23	0.1410010978862717
24	0.13465951653369163
25	0.13213279271352302
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2018424.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.174221142185566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.85218921219295	43.52426410141163
2	10.458289638874959	11.122228120531302
3	3.0102835573745628	4.802084507415602
4	1.4036567475569175	2.9855341720924984
5	0.757168325145344	2.0130917981568395
6	0.4916774848050632	1.5686740384594808
7	0.35446965177738804	1.3194053356263038
8	0.2516419100661408	1.0704690059599153
9	0.2030034797681074	0.971509673324028
>10	1.0247744284833946	9.883792706463506
>50	0.09067585137037851	3.3776558904236538
>100	0.08617212731220865	9.885140997050872
>500	0.011974800671946211	4.367015380312693
>1k	0.00402278460073193	3.109134272771778
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	2658	0.13168690027466975	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2577	0.1276738683249902	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2285	0.11320713586441698	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2193	0.1086491242672501	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2068	0.10245617372762114	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2059	0.10201028128876788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.9543604317031505E-5
7	0.0	0.0	0.0	4.9543604317031505E-5	4.9543604317031505E-5
8	0.0	0.0	0.0	9.908720863406301E-5	4.9543604317031505E-5
9	0.0	0.0	0.0	1.486308129510945E-4	4.9543604317031505E-5
10	0.0	0.0	0.0	1.486308129510945E-4	4.9543604317031505E-5
11	0.0	0.0	0.0	1.486308129510945E-4	4.9543604317031505E-5
12	0.0	0.0	0.0	1.486308129510945E-4	4.9543604317031505E-5
13	0.0	0.0	0.0	1.486308129510945E-4	4.9543604317031505E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAA	65	5.439705E-5	13.15754	19
CGTCTTA	270	0.0	13.020881	15
CGCAAGA	420	0.0	12.206811	2
CGCGCTA	125	1.4060788E-9	12.163114	16
GACCGTA	55	0.0030670986	12.091904	7
GGTCCAA	605	0.0	12.091004	11
CGGTCCA	535	0.0	12.07546	10
AAGACGG	425	0.0	12.069486	5
GGTATCA	725	0.0	12.047497	1
CGCGCCA	95	1.0395488E-6	12.0006895	10
GCGGGTA	80	2.8583965E-5	11.87863	18
CGCATCG	310	0.0	11.646408	13
AGAATTT	580	0.0	11.632575	17
ATAGGGT	335	0.0	11.620902	3
CAAGACG	465	0.0	11.438396	4
GCGCCGA	200	0.0	11.403201	19
TAACGAA	150	1.7644197E-10	11.401221	13
CGGCTAA	125	1.8297214E-8	11.401221	9
TTCTCGT	325	0.0	11.400089	11
CGCCGGT	520	0.0	11.327855	7
>>END_MODULE