FastQCFastQC Report
Thu 2 Feb 2017
SRR4062406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018424
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26950.13352001363439991No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT25370.12569212415230893No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20310.100623060367891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21800.024.1210041
CGCAATA7100.021.69009236
ATACGAA7500.020.23995440
TCTAGCG7650.020.13067228
CAATACG7750.019.87092438
GCCGGTC8150.019.7054778
AATACGA7800.019.46149439
CGGTCCA8050.019.40368310
CGCCGGT7900.019.2151477
CGAATGC7700.019.14281343
GTATAGG2300.019.1318131
TAGCGGC8050.019.1303930
GCAATAC8200.018.78044537
CTAGCGG8200.018.78044529
TACGAAT8400.018.33329241
CCGGTCC9650.018.0103239
GGTATCA10650.017.5599521
AACGCAG29150.017.5093966
TCAACGC29250.017.4495334
CAACGCA29500.017.3016555
Produced by FastQC (version 0.11.5)