##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062406_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2018424
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.333884258213338	32.0	32.0	32.0	32.0	32.0
2	31.429083780216644	32.0	32.0	32.0	32.0	32.0
3	31.509375631680957	32.0	32.0	32.0	32.0	32.0
4	31.60388302953195	32.0	32.0	32.0	32.0	32.0
5	31.533272493787234	32.0	32.0	32.0	32.0	32.0
6	35.136933567971845	36.0	36.0	36.0	36.0	36.0
7	35.14765034502166	36.0	36.0	36.0	36.0	36.0
8	35.088697419372735	36.0	36.0	36.0	36.0	36.0
9	35.22594212117969	36.0	36.0	36.0	36.0	36.0
10	35.099282905871114	36.0	36.0	36.0	36.0	36.0
11	35.232641407355445	36.0	36.0	36.0	36.0	36.0
12	35.14416247527774	36.0	36.0	36.0	36.0	36.0
13	35.19210235312303	36.0	36.0	36.0	36.0	36.0
14	35.157974241289246	36.0	36.0	36.0	36.0	36.0
15	35.11248677185765	36.0	36.0	36.0	36.0	36.0
16	35.1254969223513	36.0	36.0	36.0	36.0	36.0
17	35.09206787077443	36.0	36.0	36.0	36.0	36.0
18	35.11091772590893	36.0	36.0	36.0	36.0	36.0
19	35.10476044676441	36.0	36.0	36.0	36.0	36.0
20	35.10402274249613	36.0	36.0	36.0	36.0	36.0
21	35.10453105987642	36.0	36.0	36.0	36.0	36.0
22	35.08701095508179	36.0	36.0	36.0	36.0	36.0
23	35.04817124647745	36.0	36.0	36.0	36.0	36.0
24	35.02682786173767	36.0	36.0	36.0	36.0	36.0
25	34.9988030265197	36.0	36.0	36.0	36.0	36.0
26	34.958873854056435	36.0	36.0	36.0	32.0	36.0
27	34.94205776387915	36.0	36.0	36.0	32.0	36.0
28	34.93774895661169	36.0	36.0	36.0	32.0	36.0
29	34.91268237000749	36.0	36.0	36.0	32.0	36.0
30	34.90112979235285	36.0	36.0	36.0	32.0	36.0
31	34.907288557805494	36.0	36.0	36.0	32.0	36.0
32	34.8794539700281	36.0	36.0	36.0	32.0	36.0
33	34.868326476498495	36.0	36.0	36.0	32.0	36.0
34	34.85351046162749	36.0	36.0	36.0	32.0	36.0
35	34.838971890940655	36.0	36.0	36.0	32.0	36.0
36	34.811275529819305	36.0	36.0	36.0	32.0	36.0
37	34.817040919053674	36.0	36.0	36.0	32.0	36.0
38	34.78646112016108	36.0	36.0	36.0	32.0	36.0
39	34.79325404374899	36.0	36.0	36.0	32.0	36.0
40	34.782410930508156	36.0	36.0	36.0	32.0	36.0
41	34.75348985148809	36.0	36.0	36.0	32.0	36.0
42	34.73958197088422	36.0	36.0	36.0	32.0	36.0
43	34.733521797204155	36.0	36.0	36.0	32.0	36.0
44	34.70560199442733	36.0	36.0	36.0	32.0	36.0
45	34.682157465428475	36.0	36.0	36.0	32.0	36.0
46	34.6928445163157	36.0	36.0	36.0	32.0	36.0
47	34.66912204769662	36.0	36.0	36.0	32.0	36.0
48	34.63889599013884	36.0	36.0	36.0	32.0	36.0
49	34.63330895788001	36.0	36.0	36.0	32.0	36.0
50	34.11383980769155	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	2.0
20	5.0
21	28.0
22	122.0
23	383.0
24	1081.0
25	2398.0
26	5150.0
27	9733.0
28	16836.0
29	26962.0
30	40860.0
31	59305.0
32	90179.0
33	153450.0
34	349575.0
35	1262352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00752580491873	17.9741274441647	11.511785951599581	26.506560799316993
2	16.887393922097896	19.515807143338446	36.067527180914105	27.529271753649553
3	18.726373729817034	22.54323948058125	28.57273794719606	30.157648842405653
4	12.749886297090097	14.917425429222291	35.35952414217471	36.973164131512895
5	14.69106590092072	36.083597896180386	33.478397006773605	15.746939196125293
6	35.94727947693663	34.59067179874129	15.792772256674414	13.669276467647668
7	31.427291788048496	30.08089479712885	19.822247456431356	18.6695659583913
8	28.624065112186535	32.901560821710405	18.63478634816074	19.839587717942315
9	26.774850081053337	14.423381806795796	18.600948066412208	40.20082004573865
10	16.07229204567524	26.26905942458076	31.16535475202435	26.493293777719646
11	38.19692988192768	20.951296655212186	21.57693329052766	19.27484017233247
12	24.656860996500242	23.453793652869763	28.03419895918796	23.855146391442037
13	29.07402012659382	18.74829074565106	25.45768381668074	26.720005311074384
14	23.144988367161705	19.49654780165119	24.535726883945095	32.82273694724201
15	25.20530869628978	27.100797453855087	21.644758484837677	26.049135365017463
16	26.387369551689833	25.92393867690832	22.876610662576347	24.8120811088255
17	24.489750419138893	26.126324300543395	24.602214400938553	24.78171087937916
18	25.329316337895307	24.86682679159582	25.518473819177736	24.285383051331138
19	25.618205094667918	25.098591772590893	24.886644233322635	24.396558899418554
20	25.415423122198312	24.69560409507616	24.562480430276295	25.326492352449236
21	26.508355033432025	24.2643270194964	24.39854064359124	24.82877730348034
22	25.669730443157633	24.387393332619904	24.757830862098352	25.185045362124114
23	24.507041137045537	24.053271265105845	25.460210540500906	25.97947705734771
24	24.805590896659968	25.007877433086406	24.864300067775652	25.322231602477974
25	25.049097711878176	24.532308375247222	25.01565577896418	25.402938133910418
26	24.834920710415652	25.25618997792337	25.309251178146912	24.59963813351407
27	25.450648624867718	24.80469911178226	24.52477774739103	25.219874515958985
28	24.661716269723307	24.481823442448167	25.583276853624408	25.27318343420411
29	24.74029242617012	24.88897278272553	25.315493672290856	25.05524111881349
30	24.700013475860374	24.886792864135582	25.58094830422151	24.832245355782533
31	25.174096225570047	24.658347304629753	24.66434208075211	25.50321438904809
32	24.911614209898417	24.692235129982603	24.725577975687962	25.67057268443102
33	24.577690316801622	24.431090791627526	25.175483446490926	25.815735445079923
34	25.314651431017467	24.4595288205055	25.311480640341177	24.914339108135852
35	25.84565978208741	24.435648803224694	25.103942481857132	24.614748932830764
36	24.685299025378214	24.979538491417067	24.82293115817093	25.51223132503379
37	25.834462927511765	24.560994122146784	24.689510231745164	24.91503271859629
38	24.798307986825368	24.31491103950409	25.376333218392173	25.510447755278378
39	25.25118607388735	24.48112983198773	24.638926211737473	25.628757882387447
40	25.387430985759185	24.402058239497748	25.43994720633524	24.770563568407827
41	24.465721771045132	24.758177667328567	25.758760300115334	25.017340261510963
42	25.577777513545218	24.8711370851714	25.071590508238113	24.479494893045267
43	24.8754969223513	23.972317015651814	25.419436154147988	25.732749907848895
44	24.523638244491742	24.49782602664257	25.241277353023943	25.737258375841748
45	24.444963000836296	24.792610472328906	25.40576211935649	25.35666440747831
46	24.58738786407791	24.32193283759929	25.503351382095214	25.587327916227586
47	24.519625212542064	24.408746626080546	25.76128702393551	25.31034113744188
48	25.364888645794935	24.933116134172007	24.850576489379833	24.851418730653222
49	24.176040316603448	24.94540294804263	25.27323297780843	25.605323757545488
50	24.154389761516907	24.869601233437574	25.419832502982526	25.556176502062993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	16.5
2	20.0
3	92.0
4	164.0
5	172.0
6	180.0
7	200.5
8	221.0
9	272.5
10	324.0
11	487.5
12	651.0
13	1088.0
14	1525.0
15	2063.5
16	2602.0
17	2948.0
18	3294.0
19	3725.0
20	4156.0
21	5348.0
22	6540.0
23	7163.0
24	7786.0
25	10744.5
26	13703.0
27	17765.0
28	21827.0
29	24382.5
30	26938.0
31	28481.0
32	30024.0
33	34564.0
34	39104.0
35	45208.0
36	51312.0
37	62246.5
38	73181.0
39	80305.0
40	87429.0
41	96012.0
42	104595.0
43	106602.5
44	108610.0
45	118031.0
46	127452.0
47	136429.0
48	145406.0
49	149946.0
50	154486.0
51	150157.5
52	145829.0
53	144122.0
54	142415.0
55	144127.5
56	145840.0
57	141930.5
58	138021.0
59	127168.0
60	116315.0
61	102899.5
62	89484.0
63	78766.5
64	68049.0
65	57701.5
66	47354.0
67	41208.0
68	35062.0
69	32336.0
70	29610.0
71	22989.0
72	16368.0
73	14535.5
74	12703.0
75	9515.0
76	6327.0
77	5715.0
78	5103.0
79	4288.0
80	3473.0
81	2762.0
82	2051.0
83	1737.0
84	1423.0
85	1164.5
86	906.0
87	639.0
88	372.0
89	235.0
90	98.0
91	67.0
92	36.0
93	31.0
94	26.0
95	16.0
96	6.0
97	5.0
98	4.0
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014912624899426484
2	0.004756186014435024
3	6.936104604384412E-4
4	2.97261625902189E-4
5	0.0
6	3.468052302192206E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.486308129510945E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2018424.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.14537236322741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7043945575729	46.43469028510208
2	10.269756812841674	11.531986406735745
3	2.7690573560776004	4.664092690563326
4	1.2611162885087854	2.832233744466283
5	0.7113919531611813	1.9970683053219078
6	0.4667386968955846	1.5723130760118078
7	0.31289877796661447	1.229747288065407
8	0.23185055341908714	1.0413868523467995
9	0.17598807874448832	0.8892824591338454
>10	0.9325642584664093	9.430964056309922
>50	0.07741074671866607	3.076529748425284
>100	0.07307193778731591	8.794456691729453
>500	0.01083043731905909	4.084944702363105
>1k	0.002929544520729097	2.42030369342521
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2695	0.13352001363439991	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2537	0.12569212415230893	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2031	0.100623060367891	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.9543604317031505E-5	0.0
5	0.0	0.0	0.0	4.9543604317031505E-5	0.0
6	0.0	0.0	0.0	4.9543604317031505E-5	0.0
7	0.0	0.0	0.0	4.9543604317031505E-5	0.0
8	0.0	0.0	0.0	9.908720863406301E-5	0.0
9	0.0	0.0	0.0	9.908720863406301E-5	0.0
10	0.0	0.0	0.0	9.908720863406301E-5	0.0
11	0.0	0.0	0.0	9.908720863406301E-5	0.0
12	0.0	0.0	0.0	9.908720863406301E-5	0.0
13	0.0	0.0	0.0	9.908720863406301E-5	0.0
14	0.0	0.0	0.0	9.908720863406301E-5	4.9543604317031505E-5
15	0.0	0.0	0.0	9.908720863406301E-5	4.9543604317031505E-5
16	4.9543604317031505E-5	0.0	0.0	1.486308129510945E-4	4.9543604317031505E-5
17	4.9543604317031505E-5	0.0	0.0	1.486308129510945E-4	4.9543604317031505E-5
18	4.9543604317031505E-5	0.0	0.0	1.486308129510945E-4	1.486308129510945E-4
19	4.9543604317031505E-5	0.0	0.0	1.486308129510945E-4	1.486308129510945E-4
20	4.9543604317031505E-5	0.0	0.0	2.477180215851575E-4	1.9817441726812602E-4
21	4.9543604317031505E-5	4.9543604317031505E-5	0.0	3.9634883453625204E-4	1.9817441726812602E-4
22	4.9543604317031505E-5	4.9543604317031505E-5	0.0	4.95436043170315E-4	1.9817441726812602E-4
23	4.9543604317031505E-5	4.9543604317031505E-5	0.0	8.422412733895356E-4	2.97261625902189E-4
24	4.9543604317031505E-5	4.9543604317031505E-5	0.0	0.001189046503608756	2.97261625902189E-4
25	4.9543604317031505E-5	4.9543604317031505E-5	0.0	0.0014863081295109451	2.97261625902189E-4
26	4.9543604317031505E-5	4.9543604317031505E-5	0.0	0.0017835697554131342	4.4589243885328355E-4
27	4.9543604317031505E-5	4.9543604317031505E-5	0.0	0.0035175959065092367	4.4589243885328355E-4
28	4.9543604317031505E-5	4.9543604317031505E-5	0.0	0.01020598248930849	4.4589243885328355E-4
29	4.9543604317031505E-5	9.908720863406301E-5	0.0	0.02209644752539605	4.4589243885328355E-4
30	4.9543604317031505E-5	9.908720863406301E-5	0.0	0.04671961887096071	4.4589243885328355E-4
31	4.9543604317031505E-5	9.908720863406301E-5	0.0	0.10478472313052163	4.95436043170315E-4
32	4.9543604317031505E-5	9.908720863406301E-5	0.0	0.16403887389369132	4.95436043170315E-4
33	4.9543604317031505E-5	9.908720863406301E-5	0.0	0.22809875427561305	4.95436043170315E-4
34	4.9543604317031505E-5	9.908720863406301E-5	0.0	0.30350412004613503	5.94523251804378E-4
35	4.9543604317031505E-5	9.908720863406301E-5	0.0	0.3864896572771628	5.94523251804378E-4
36	4.9543604317031505E-5	9.908720863406301E-5	0.0	0.512726761076959	5.94523251804378E-4
37	4.9543604317031505E-5	9.908720863406301E-5	0.0	0.690390126157834	5.94523251804378E-4
38	4.9543604317031505E-5	9.908720863406301E-5	0.0	0.929239842570243	5.94523251804378E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2180	0.0	24.121004	1
CGCAATA	710	0.0	21.690092	36
ATACGAA	750	0.0	20.239954	40
TCTAGCG	765	0.0	20.130672	28
CAATACG	775	0.0	19.870924	38
GCCGGTC	815	0.0	19.705477	8
AATACGA	780	0.0	19.461494	39
CGGTCCA	805	0.0	19.403683	10
CGCCGGT	790	0.0	19.215147	7
CGAATGC	770	0.0	19.142813	43
GTATAGG	230	0.0	19.131813	1
TAGCGGC	805	0.0	19.13039	30
GCAATAC	820	0.0	18.780445	37
CTAGCGG	820	0.0	18.780445	29
TACGAAT	840	0.0	18.333292	41
CCGGTCC	965	0.0	18.010323	9
GGTATCA	1065	0.0	17.559952	1
AACGCAG	2915	0.0	17.509396	6
TCAACGC	2925	0.0	17.449533	4
CAACGCA	2950	0.0	17.301655	5
>>END_MODULE