FastQCFastQC Report
Thu 2 Feb 2017
SRR4062405_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062405_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772406
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG138380.7807466235162824No Hit
TCCATGTACTCTGCGTTGATACCAC119460.6739990724472835No Hit
GAGTACATGGAAGCAGTGGTATCAA67050.37829932870911065No Hit
CATGTACTCTGCGTTGATACCACTG57420.3239664049884733No Hit
GCTTCCATGTACTCTGCGTTGATAC38910.219532093662513No Hit
GTATCAACGCAGAGTACTTTTTTTT37580.21202816961802207No Hit
GCGTTGATACCACTGCTTCCATGTA34060.19216816011681295No Hit
CATGGAAGCAGTGGTATCAACGCAG31300.17659610721245583No Hit
CCCATGTACTCTGCGTTGATACCAC30720.17332371928327933No Hit
GTACATGGGAAGCAGTGGTATCAAC29250.16502990849726307No Hit
TATCAACGCAGAGTACTTTTTTTTT25280.14263097732686528No Hit
GTATCAACGCAGAGTACATGGAAGC25190.14212319299302756No Hit
ACTCTGCGTTGATACCACTGCTTCC24280.1369889291731127No Hit
GTCTTGCGCCGGTCCAAGAATTTCA24010.13546557617159952No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23110.1303877328332222No Hit
ACGCAGAGTACATGGAAGCAGTGGT22900.12920290272093415No Hit
GCGCAAGACGGACCAGAGCGAAAGC22330.12598693527329516No Hit
CAGTGGTATCAACGCAGAGTACATG21450.1210219328979929No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20330.11470283896579002No Hit
GAATAACGCCGCCGCATCGCCAGTC19700.11114834862892588No Hit
GAATAGGACCGCGGTTCTATTTTGT18780.10595766432747349No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18590.1048856751782605No Hit
GAACTACGACGGTATCTGATCGTCT18350.1035315836213599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTTC4100.013.67504318
CGAACGA1550.013.48736116
CGGTCCA4550.013.15576310
AGAATTT4350.013.10757917
CGGACAT2100.012.6656495
CGCAATA1501.8189894E-1212.6592122
GTATTAC1001.4566285E-712.3409891
ACGAACG1700.012.29590915
TTAGTAC701.0956332E-412.208823
CGAGCCG3850.012.09267115
GGTTCTA3550.012.04298813
CGGTTCT3550.012.04264712
AATCGCT951.0382028E-612.00174915
ACGGTAT3250.011.9860249
AAGACGG3900.011.9349395
AACGGAC1209.993528E-911.87673115
CTAAACG802.8659286E-511.87538812
TTACTCT1201.00444595E-811.8727054
GTTCAAA4650.011.84081651
CCTATAC658.05457E-411.6870773
Produced by FastQC (version 0.11.5)