##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062405_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1772406
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178413975127594	32.0	32.0	32.0	32.0	32.0
2	30.823836073676123	32.0	32.0	32.0	32.0	32.0
3	30.809610777666066	32.0	32.0	32.0	32.0	32.0
4	30.889954107580316	32.0	32.0	32.0	32.0	32.0
5	30.66153748069009	32.0	32.0	32.0	32.0	32.0
6	34.32075720799862	36.0	36.0	36.0	32.0	36.0
7	34.19770244515083	36.0	36.0	36.0	32.0	36.0
8	34.18872820335747	36.0	36.0	36.0	32.0	36.0
9	34.370095226488736	36.0	36.0	36.0	32.0	36.0
10	34.018650354377044	36.0	36.0	36.0	32.0	36.0
11	34.35827682822107	36.0	36.0	36.0	32.0	36.0
12	34.114946011241216	36.0	36.0	36.0	32.0	36.0
13	34.24493767229404	36.0	36.0	36.0	32.0	36.0
14	34.1446102078192	36.0	36.0	36.0	32.0	36.0
15	34.081508412858	36.0	36.0	36.0	32.0	36.0
16	34.07048836440409	36.0	36.0	36.0	32.0	36.0
17	33.98717223931763	36.0	36.0	36.0	32.0	36.0
18	34.00436073901803	36.0	36.0	36.0	32.0	36.0
19	33.99952155431656	36.0	36.0	36.0	32.0	36.0
20	33.99146527375782	36.0	36.0	36.0	32.0	36.0
21	33.952581406291785	36.0	36.0	36.0	32.0	36.0
22	33.90074678149363	36.0	36.0	36.0	32.0	36.0
23	33.829422265553156	36.0	36.0	36.0	32.0	36.0
24	33.804628849146305	36.0	36.0	36.0	32.0	36.0
25	33.29813259490207	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	64.0
5	149.0
6	305.0
7	70.0
8	271.0
9	283.0
10	207.0
11	71.0
12	121.0
13	106.0
14	252.0
15	364.0
16	562.0
17	769.0
18	984.0
19	1458.0
20	2227.0
21	3167.0
22	4860.0
23	7324.0
24	10130.0
25	14616.0
26	20610.0
27	27059.0
28	37288.0
29	50294.0
30	65886.0
31	91667.0
32	133444.0
33	190227.0
34	410750.0
35	696816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.37151993120691	16.961345076458397	11.158451500927342	25.508683491407353
2	16.470788035812216	19.791170622606295	36.552870867929464	27.185170473652022
3	19.859348913209683	23.30532310874561	27.967662888775163	28.867665089269543
4	12.756211022406402	14.795572890216075	35.017199233482984	37.43101685389455
5	14.43651280106287	36.39218966410544	33.702053159318794	15.469244375512895
6	35.3853074969618	34.02868914225527	16.567406727549105	14.018596633233821
7	30.44339836750271	30.453617001096383	20.355010009744397	18.747974621656503
8	28.76954290757695	32.51885611416949	19.611770439056507	19.099830539197058
9	26.757070863367044	14.782232542800156	18.515485761141367	39.94521083269144
10	15.931947774661731	26.76223801898436	31.580852968671007	25.7249612376829
11	37.76013674000927	21.223283081982437	20.999770104164842	20.01681007384345
12	23.960450630514387	24.032309272363463	28.5339820195216	23.473258077600555
13	29.877148794618925	19.175102366590167	25.181192677392588	25.76655616139832
14	23.61413238420012	19.49453120542818	24.567069061642634	32.324267348729066
15	25.64696925025106	26.47888771783567	23.100210902543143	24.773932129370127
16	26.476973892297824	25.619651264718907	23.76905663446506	24.134318208518213
17	24.102560336753818	25.906082197847386	25.81429057218098	24.177066893217813
18	25.481130988292875	24.1251855718324	26.976890799026553	23.416792640848175
19	25.004109786375846	26.47374679761491	25.008742122909446	23.513401293099793
20	24.805758419112188	24.092225987334686	26.48593750264779	24.61607809090534
21	25.855113792034025	25.095144455183956	23.93341328649878	25.116328466283242
22	24.66857604232644	25.323259483926158	24.90480901629109	25.10335545745631
23	23.57273402653605	24.711179659274197	25.27845337265437	26.437632941535384
24	23.819969550517047	26.32987693098588	24.983377067980307	24.866776450516763
25	25.124011761416114	24.66044701289971	24.600001694728547	25.615539530955626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	165.0
1	165.0
2	481.5
3	798.0
4	798.0
5	798.0
6	2481.0
7	4164.0
8	4164.0
9	4164.0
10	4438.0
11	4712.0
12	4712.0
13	4712.0
14	4854.5
15	4997.0
16	4997.0
17	4997.0
18	8111.5
19	11226.0
20	11226.0
21	11226.0
22	17946.5
23	24667.0
24	24667.0
25	24667.0
26	36843.5
27	49020.0
28	49020.0
29	49020.0
30	60361.5
31	71703.0
32	71703.0
33	71703.0
34	87733.5
35	103764.0
36	103764.0
37	103764.0
38	117871.5
39	131979.0
40	131979.0
41	131979.0
42	154663.5
43	177348.0
44	177348.0
45	177348.0
46	227102.0
47	276856.0
48	276856.0
49	276856.0
50	258393.5
51	239931.0
52	239931.0
53	239931.0
54	218526.0
55	197121.0
56	197121.0
57	197121.0
58	182045.0
59	166969.0
60	166969.0
61	166969.0
62	148155.5
63	129342.0
64	129342.0
65	129342.0
66	106961.5
67	84581.0
68	84581.0
69	84581.0
70	63643.0
71	42705.0
72	42705.0
73	42705.0
74	33427.0
75	24149.0
76	24149.0
77	24149.0
78	19800.0
79	15451.0
80	15451.0
81	15451.0
82	10693.0
83	5935.0
84	5935.0
85	5935.0
86	4366.5
87	2798.0
88	2798.0
89	2798.0
90	1940.5
91	1083.0
92	1083.0
93	1083.0
94	628.5
95	174.0
96	174.0
97	174.0
98	471.0
99	768.0
100	768.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007616765007565986
2	0.0028210240768762915
3	0.0043443770783894884
4	0.013202392679781044
5	0.033852288922515496
6	0.04485428282233303
7	0.06386798510047922
8	0.08434861989860111
9	0.09128833912771679
10	0.11289738355658918
11	0.11504136185501515
12	0.12773597020095845
13	0.11583124859654052
14	0.11786238593189145
15	0.10832732455204959
16	0.10686039203207391
17	0.11803164737650403
18	0.12423790034563187
19	0.12643829912559537
20	0.11616977148576567
21	0.1242943208271694
22	0.1434772845499282
23	0.13320875691009848
24	0.1275667087563459
25	0.12474568467946962
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1772406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.84091062533175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.81985915952482	37.22972926926732
2	13.136075043927162	12.568835842883406
3	3.9724858102689553	5.70142015828427
4	1.6702373623928186	3.1962270550929874
5	0.8819105806618874	2.109570263448989
6	0.5191193363621515	1.4901085064869928
7	0.35078911118684986	1.1747449361640754
8	0.25097708087665943	0.9605577676181537
9	0.18024408759061086	0.7760737156650193
>10	0.9938762046930344	8.787636191005427
>50	0.101072677446964	3.4019316967438353
>100	0.10161338921760249	10.361893350117402
>500	0.015241739699547861	5.00974034271391
>1k	0.006025804067263108	5.070505234942297
>5k	2.363060418534552E-4	0.7035726637830036
>10k+	2.363060418534552E-4	1.457453005782997
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	13838	0.7807466235162824	No Hit
TCCATGTACTCTGCGTTGATACCAC	11946	0.6739990724472835	No Hit
GAGTACATGGAAGCAGTGGTATCAA	6705	0.37829932870911065	No Hit
CATGTACTCTGCGTTGATACCACTG	5742	0.3239664049884733	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3891	0.219532093662513	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3758	0.21202816961802207	No Hit
GCGTTGATACCACTGCTTCCATGTA	3406	0.19216816011681295	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3130	0.17659610721245583	No Hit
CCCATGTACTCTGCGTTGATACCAC	3072	0.17332371928327933	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2925	0.16502990849726307	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2528	0.14263097732686528	No Hit
GTATCAACGCAGAGTACATGGAAGC	2519	0.14212319299302756	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2428	0.1369889291731127	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2401	0.13546557617159952	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2311	0.1303877328332222	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2290	0.12920290272093415	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2233	0.12598693527329516	No Hit
CAGTGGTATCAACGCAGAGTACATG	2145	0.1210219328979929	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2033	0.11470283896579002	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1970	0.11114834862892588	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1878	0.10595766432747349	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1859	0.1048856751782605	No Hit
GAACTACGACGGTATCTGATCGTCT	1835	0.1035315836213599	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1284096307505166E-4
2	0.0	0.0	0.0	0.0	1.1284096307505166E-4
3	0.0	0.0	0.0	0.0	1.1284096307505166E-4
4	0.0	0.0	0.0	0.0	1.1284096307505166E-4
5	0.0	0.0	0.0	0.0	1.1284096307505166E-4
6	0.0	0.0	0.0	0.0	1.1284096307505166E-4
7	0.0	0.0	0.0	0.0	1.1284096307505166E-4
8	0.0	0.0	0.0	0.0	1.1284096307505166E-4
9	0.0	0.0	0.0	0.0	1.1284096307505166E-4
10	0.0	0.0	0.0	0.0	2.2568192615010331E-4
11	0.0	0.0	0.0	0.0	2.2568192615010331E-4
12	0.0	5.642048153752583E-5	0.0	0.0	4.5136385230020663E-4
13	0.0	5.642048153752583E-5	0.0	0.0	7.334662599878357E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATTTC	410	0.0	13.675043	18
CGAACGA	155	0.0	13.487361	16
CGGTCCA	455	0.0	13.155763	10
AGAATTT	435	0.0	13.107579	17
CGGACAT	210	0.0	12.665649	5
CGCAATA	150	1.8189894E-12	12.659212	2
GTATTAC	100	1.4566285E-7	12.340989	1
ACGAACG	170	0.0	12.295909	15
TTAGTAC	70	1.0956332E-4	12.20882	3
CGAGCCG	385	0.0	12.092671	15
GGTTCTA	355	0.0	12.042988	13
CGGTTCT	355	0.0	12.042647	12
AATCGCT	95	1.0382028E-6	12.001749	15
ACGGTAT	325	0.0	11.986024	9
AAGACGG	390	0.0	11.934939	5
AACGGAC	120	9.993528E-9	11.876731	15
CTAAACG	80	2.8659286E-5	11.875388	12
TTACTCT	120	1.00444595E-8	11.872705	4
GTTCAAA	465	0.0	11.8408165	1
CCTATAC	65	8.05457E-4	11.687077	3
>>END_MODULE