FastQCFastQC Report
Thu 2 Feb 2017
SRR4062405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772406
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA62180.3508225542003356No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC54810.3092406593071791No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA27110.15295592544823253No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC25130.1417846701038024No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC23670.13354727979932363No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22590.12745386779327084No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20770.11718534015344115No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19240.1085530064781997No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG17870.10082340050755864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG500.00258057222.0004942
TCGTATA654.9336045E-420.30757544
ATACTAG1001.1216744E-619.8043561
CGATTAA1303.5903213E-818.61527641
TCGCATG600.007413676518.3332311
CGCAATA5950.018.11754236
TAGCGGC6000.017.96656230
AATACGA6200.017.38699739
CAATACG6250.017.247938
CTAGCGG6350.016.9762829
GCGAAAG7000.016.9713318
ACGATTA1307.321978E-716.9229840
CGCCGGT6700.016.7461727
GAGCGAA7100.016.73229816
AGCGAAA7100.016.73229817
GATATAC2400.016.503631
GTATAGA2007.2759576E-1116.503631
TATATCA4000.016.4999054
CGAATGC6550.016.4579243
CGAAAGC7150.016.30759819
Produced by FastQC (version 0.11.5)