##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062405_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1772406
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277181413287927	32.0	32.0	32.0	32.0	32.0
2	31.412135819896797	32.0	32.0	32.0	32.0	32.0
3	31.502816510438354	32.0	32.0	32.0	32.0	32.0
4	31.608709855416873	32.0	32.0	32.0	32.0	32.0
5	31.53739944459678	32.0	32.0	32.0	32.0	32.0
6	35.14741769098051	36.0	36.0	36.0	36.0	36.0
7	35.158545502554155	36.0	36.0	36.0	36.0	36.0
8	35.10638533157753	36.0	36.0	36.0	36.0	36.0
9	35.24062263386605	36.0	36.0	36.0	36.0	36.0
10	35.11276705224424	36.0	36.0	36.0	36.0	36.0
11	35.24123197506666	36.0	36.0	36.0	36.0	36.0
12	35.149716261398346	36.0	36.0	36.0	36.0	36.0
13	35.18761051361821	36.0	36.0	36.0	36.0	36.0
14	35.149330909509445	36.0	36.0	36.0	36.0	36.0
15	35.11362746458769	36.0	36.0	36.0	36.0	36.0
16	35.11306551659157	36.0	36.0	36.0	36.0	36.0
17	35.08687117962815	36.0	36.0	36.0	36.0	36.0
18	35.099519523179225	36.0	36.0	36.0	36.0	36.0
19	35.08283034474043	36.0	36.0	36.0	36.0	36.0
20	35.073986998464235	36.0	36.0	36.0	36.0	36.0
21	35.04868636192836	36.0	36.0	36.0	36.0	36.0
22	35.02908475823259	36.0	36.0	36.0	36.0	36.0
23	34.972761884128126	36.0	36.0	36.0	36.0	36.0
24	34.93987438543991	36.0	36.0	36.0	32.0	36.0
25	34.91163254920148	36.0	36.0	36.0	32.0	36.0
26	34.860065357485816	36.0	36.0	36.0	32.0	36.0
27	34.82979520493612	36.0	36.0	36.0	32.0	36.0
28	34.80306995124142	36.0	36.0	36.0	32.0	36.0
29	34.76411725078791	36.0	36.0	36.0	32.0	36.0
30	34.75109145421534	36.0	36.0	36.0	32.0	36.0
31	34.73753981875485	36.0	36.0	36.0	32.0	36.0
32	34.69209481349081	36.0	36.0	36.0	32.0	36.0
33	34.674100629314054	36.0	36.0	36.0	32.0	36.0
34	34.65025282017777	36.0	36.0	36.0	32.0	36.0
35	34.59113543962275	36.0	36.0	36.0	32.0	36.0
36	34.55525539859378	36.0	36.0	36.0	32.0	36.0
37	34.51834850480082	36.0	36.0	36.0	32.0	36.0
38	34.460121439444464	36.0	36.0	36.0	32.0	36.0
39	34.3764605852158	36.0	36.0	36.0	32.0	36.0
40	34.325014697535444	36.0	36.0	36.0	32.0	36.0
41	34.30495890896329	36.0	36.0	36.0	32.0	36.0
42	34.194130464464685	36.0	36.0	36.0	32.0	36.0
43	34.22147521504667	36.0	36.0	36.0	32.0	36.0
44	34.192701333667344	36.0	36.0	36.0	32.0	36.0
45	34.06858078792331	36.0	36.0	36.0	32.0	36.0
46	34.14909394348699	36.0	36.0	36.0	32.0	36.0
47	34.075737726006345	36.0	36.0	36.0	32.0	36.0
48	34.06174657499467	36.0	36.0	36.0	32.0	36.0
49	34.09680118437875	36.0	36.0	36.0	32.0	36.0
50	33.53219860460865	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	49.0
22	135.0
23	468.0
24	1312.0
25	3154.0
26	6747.0
27	12824.0
28	21902.0
29	32298.0
30	45568.0
31	64476.0
32	94937.0
33	141372.0
34	305163.0
35	1041995.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.931255625245115	17.47994661836362	11.651924644992368	25.9368731113989
2	15.800134624813733	20.263291379215982	36.270610545535185	27.665963450435104
3	19.302514787891646	23.50259197154586	28.32171817133417	28.873175069228324
4	12.521799475853431	15.302766579873111	35.18857146081172	36.986862483461735
5	14.231897206396276	36.542812425595486	33.58536362436146	15.63992674364677
6	35.603213497637945	34.2217525512032	16.24295658031854	13.932077370840313
7	30.444886781019697	30.533241255107463	20.28011640673751	18.741755557135328
8	28.765305466129092	32.72749020258338	19.41806786932565	19.089136461961875
9	26.857164780529967	14.58824896778729	18.355557361010966	40.19902889067178
10	15.92727625611739	26.782294801529673	31.593269262234497	25.697159680118435
11	37.9819860686547	21.13494312251256	20.907681422879406	19.97538938595333
12	23.956474983722693	23.97475521974085	28.507068922131833	23.56170087440462
13	30.063766428233713	19.049924227293292	25.169233234371806	25.717076110101182
14	23.684246160304127	19.365766082940365	24.5473666868652	32.40262106989031
15	25.73936220030851	26.58177641014531	22.957381096656185	24.721480292890003
16	26.548544746519703	25.578958771297323	23.705403840880702	24.167092641302276
17	24.208843797640046	25.99556760697041	25.673519498354214	24.12206909703533
18	25.663533073122075	24.012500521889454	26.83578141802725	23.488184986961226
19	25.11540809498501	26.437001454520015	24.927979255317346	23.519611195177628
20	24.893167818208696	24.013685352001744	26.53240848879997	24.560738340989595
21	26.044314903018833	24.987672124784048	23.904173197337407	25.06383977485971
22	24.67707737391997	25.24720633985667	24.97604950558732	25.09966678063604
23	23.67826558926115	24.496136889629124	25.323261148969255	26.502336372140466
24	23.980397267894602	26.166634506992192	24.863547065401495	24.989421159711714
25	25.37900458472833	24.35395727615456	24.606382510553452	25.66065562856366
26	23.8946945564391	25.181081535494688	25.584770080895687	25.339453827170523
27	25.608523103622982	25.260352312054913	25.023950494412677	24.10717408990942
28	24.504994905230518	24.043362525290483	25.776994661494037	25.674647907984966
29	23.834324641193945	25.433788872301268	26.275920979730376	24.455965506774408
30	25.137637764710796	24.77265366964454	25.9958497093781	24.093858856266568
31	24.256970468391554	24.989421159711714	24.98236859951952	25.771239772377207
32	24.171154915972977	25.208840412411153	25.243200485667504	25.376804185948366
33	23.853338343472092	24.640968265735953	25.669400803201974	25.836292587589977
34	24.237279720334957	25.367043442642373	26.31417406621282	24.08150277080985
35	25.814683543161106	24.710534719471724	25.475145085268274	23.9996366520989
36	24.3261983992381	25.663984436974374	25.200772283551288	24.809044880236243
37	25.68390086695712	25.115351674503472	25.292229884123614	23.908517574415793
38	24.437177486422414	24.96741717191208	25.07709858802103	25.51830675364448
39	24.591769605835232	24.845041147457188	25.357451960780995	25.20573728592659
40	25.45223836976404	25.144577483939912	24.950491027450823	24.452693118845232
41	23.70861980832834	25.30644784547107	26.087702253321194	24.897230092879397
42	26.004256361127194	25.419514490472274	25.16009311636273	23.41613603203781
43	24.87206655811366	23.86986954456259	25.216513597900253	26.041550299423495
44	24.444624989985364	24.720577565185405	26.0743305991968	24.760466845632433
45	24.823601364472925	24.98056314411032	25.439995125270393	24.755840366146355
46	24.23562812746311	24.67091287929438	26.129666898555236	24.96379209468727
47	24.87093814848291	24.216404142166073	26.51790842504483	24.39474928430619
48	24.950942391303123	26.53601939961837	24.384255074740214	24.128783134338295
49	23.76819983683197	25.042117889467765	26.46701715069798	24.72266512300229
50	23.68205912305596	26.500094504359893	24.528479664636468	25.289366707947675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	15.0
2	19.0
3	92.5
4	166.0
5	201.5
6	237.0
7	230.0
8	223.0
9	278.0
10	333.0
11	565.5
12	798.0
13	1402.5
14	2007.0
15	2815.5
16	3624.0
17	4448.5
18	5273.0
19	5833.5
20	6394.0
21	7538.0
22	8682.0
23	9328.5
24	9975.0
25	11424.0
26	12873.0
27	15962.0
28	19051.0
29	21175.5
30	23300.0
31	24724.5
32	26149.0
33	30140.5
34	34132.0
35	39516.5
36	44901.0
37	54572.0
38	64243.0
39	68893.0
40	73543.0
41	81099.0
42	88655.0
43	92709.0
44	96763.0
45	112032.5
46	127302.0
47	140120.0
48	152938.0
49	149551.5
50	146165.0
51	134805.0
52	123445.0
53	119940.5
54	116436.0
55	118634.5
56	120833.0
57	117009.0
58	113185.0
59	104279.0
60	95373.0
61	84404.5
62	73436.0
63	63871.0
64	54306.0
65	46048.5
66	37791.0
67	32807.5
68	27824.0
69	25628.0
70	23432.0
71	18270.5
72	13109.0
73	11812.0
74	10515.0
75	7641.5
76	4768.0
77	4218.0
78	3668.0
79	3174.5
80	2681.0
81	2117.5
82	1554.0
83	1327.0
84	1100.0
85	897.0
86	694.0
87	518.0
88	342.0
89	214.0
90	86.0
91	66.0
92	46.0
93	28.0
94	10.0
95	8.0
96	6.0
97	4.5
98	3.0
99	4.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01472574568129424
2	0.0041186951522393855
3	5.642048153752583E-4
4	5.642048153752583E-5
5	0.0
6	3.949433707626808E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.6926144461257747E-4
47	0.0
48	0.0
49	0.0
50	5.642048153752583E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1772406.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.09357976548602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61087037806239	40.95121646473875
2	12.905456740505173	13.445828802430709
3	3.750519060255788	5.861338914822317
4	1.5312956796494823	3.190826945294577
5	0.8341258437114942	2.1726300586919023
6	0.5024341314808589	1.5704155503120492
7	0.3387489833364733	1.2352653028741107
8	0.2444444893323235	1.0187190802613513
9	0.17485549734818484	0.8197963918687255
>10	0.9101906225127638	8.697189730956545
>50	0.09236053068191535	3.3817686575265746
>100	0.08911905485335933	9.941501873084526
>500	0.011657005208844471	4.172126511807561
>1k	0.003704095113090767	2.8774245215218213
>5k	2.178879478288686E-4	0.6639511938084808
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6218	0.3508225542003356	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5481	0.3092406593071791	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2711	0.15295592544823253	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2513	0.1417846701038024	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2367	0.13354727979932363	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2259	0.12745386779327084	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2077	0.11718534015344115	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1924	0.1085530064781997	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1787	0.10082340050755864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6926144461257747E-4
2	0.0	0.0	0.0	0.0	1.6926144461257747E-4
3	0.0	0.0	0.0	0.0	1.6926144461257747E-4
4	0.0	0.0	0.0	0.0	1.6926144461257747E-4
5	0.0	0.0	0.0	0.0	1.6926144461257747E-4
6	0.0	0.0	0.0	0.0	1.6926144461257747E-4
7	0.0	0.0	0.0	0.0	2.2568192615010331E-4
8	0.0	0.0	0.0	0.0	2.2568192615010331E-4
9	0.0	0.0	0.0	0.0	2.2568192615010331E-4
10	0.0	0.0	0.0	0.0	2.2568192615010331E-4
11	0.0	0.0	0.0	0.0	2.2568192615010331E-4
12	0.0	0.0	0.0	0.0	5.077843338377325E-4
13	0.0	0.0	0.0	0.0	5.642048153752583E-4
14	0.0	0.0	0.0	0.0	5.642048153752583E-4
15	0.0	0.0	0.0	0.0	5.642048153752583E-4
16	0.0	0.0	0.0	0.0	6.20625296912784E-4
17	0.0	0.0	0.0	5.642048153752583E-5	6.20625296912784E-4
18	0.0	0.0	0.0	1.6926144461257747E-4	7.898867415253616E-4
19	0.0	0.0	0.0	2.8210240768762916E-4	7.898867415253616E-4
20	0.0	0.0	0.0	4.5136385230020663E-4	8.463072230628874E-4
21	0.0	0.0	0.0	6.770457784503099E-4	8.463072230628874E-4
22	0.0	0.0	0.0	0.0011284096307505166	9.027277046004133E-4
23	0.0	0.0	0.0	0.0020875578168884556	9.027277046004133E-4
24	0.0	0.0	0.0	0.004287956596851963	9.027277046004133E-4
25	0.0	0.0	0.0	0.005642048153752583	9.027277046004133E-4
26	0.0	0.0	0.0	0.007786026452178564	9.591481861379391E-4
27	0.0	0.0	0.0	0.010324948121367226	0.001015568667675465
28	0.0	0.0	0.0	0.016982564942795274	0.001015568667675465
29	0.0	0.0	0.0	0.02973359377027611	0.001015568667675465
30	0.0	0.0	0.0	0.05247104782989902	0.001015568667675465
31	0.0	0.0	0.0	0.09518135235380606	0.001015568667675465
32	0.0	0.0	0.0	0.1509812085944191	0.001015568667675465
33	0.0	0.0	0.0	0.20068765282897935	0.001015568667675465
34	0.0	5.642048153752583E-5	0.0	0.26495058130022126	0.0010719891492129907
35	0.0	5.642048153752583E-5	0.0	0.3318088519221894	0.0010719891492129907
36	0.0	5.642048153752583E-5	0.0	0.43460696928356146	0.0010719891492129907
37	0.0	5.642048153752583E-5	0.0	0.5918508513286459	0.0010719891492129907
38	0.0	5.642048153752583E-5	0.0	0.7756123596963675	0.0011284096307505166
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAACG	50	0.002580572	22.000494	2
TCGTATA	65	4.9336045E-4	20.307575	44
ATACTAG	100	1.1216744E-6	19.804356	1
CGATTAA	130	3.5903213E-8	18.615276	41
TCGCATG	60	0.0074136765	18.33323	11
CGCAATA	595	0.0	18.117542	36
TAGCGGC	600	0.0	17.966562	30
AATACGA	620	0.0	17.386997	39
CAATACG	625	0.0	17.2479	38
CTAGCGG	635	0.0	16.97628	29
GCGAAAG	700	0.0	16.97133	18
ACGATTA	130	7.321978E-7	16.92298	40
CGCCGGT	670	0.0	16.746172	7
GAGCGAA	710	0.0	16.732298	16
AGCGAAA	710	0.0	16.732298	17
GATATAC	240	0.0	16.50363	1
GTATAGA	200	7.2759576E-11	16.50363	1
TATATCA	400	0.0	16.499905	4
CGAATGC	655	0.0	16.45792	43
CGAAAGC	715	0.0	16.307598	19
>>END_MODULE