FastQCFastQC Report
Thu 2 Feb 2017
SRR4062404_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062404_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658232
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34430.2076307778404952No Hit
TATCAACGCAGAGTACTTTTTTTTT22880.13797828048186261No Hit
GATTAAGAGGGACGGCCGGGGGCAT19140.11542413848001967No Hit
GCGCAAGACGGACCAGAGCGAAAGC19030.11476078136231842No Hit
GAATAGGACCGCGGTTCTATTTTGT18860.11373559308950737No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG18580.11204704769899507No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18510.111624911351367No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18320.11047911269351936No Hit
GTACATGGAAGCAGTGGTATCAACG17980.10842873614789728No Hit
GAATAACGCCGCCGCATCGCCAGTC17920.1080669049927875No Hit
GTATCTGATCGTCTTCGAACCTCCG17840.10758446345264112No Hit
GTCCTATTCCATTATTCCTAGCTGC16980.10239821689606762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGC400.005282636714.2486414
GTATTAC903.6670826E-813.7142871
AATTTCG707.2477415E-613.57423418
CGAACGA1800.013.19717216
ATTTCGG604.0851478E-412.66928519
AAAGCGT751.4769577E-512.66813810
GTACTAA701.0968902E-412.2072241
ATCGCCA3550.012.04474216
TAAGACC951.0410968E-611.9988564
CGCCAGT3600.011.87745518
GATAACG2000.011.87566111
CGGAATT1201.0009899E-811.87530215
CGCATCG3550.011.77530313
GCATCGC3550.011.77494714
CTTACAC1302.6284397E-911.6883693
CGATAAC2050.011.58671110
ATAACGA2050.011.58566112
CGGTCCA4300.011.26874910
CAACGGA1104.966696E-711.22755814
GTTTTCG2200.011.22755815
Produced by FastQC (version 0.11.5)