##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062404_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1658232
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251458179555094	32.0	32.0	32.0	32.0	32.0
2	31.02104470303311	32.0	32.0	32.0	32.0	32.0
3	31.01232517524689	32.0	32.0	32.0	32.0	32.0
4	31.033146749067683	32.0	32.0	32.0	32.0	32.0
5	30.921125029549543	32.0	32.0	32.0	32.0	32.0
6	34.498521919731374	36.0	36.0	36.0	32.0	36.0
7	34.4301376405714	36.0	36.0	36.0	32.0	36.0
8	34.44451198626007	36.0	36.0	36.0	32.0	36.0
9	34.5460592968897	36.0	36.0	36.0	32.0	36.0
10	34.312754186386464	36.0	36.0	36.0	32.0	36.0
11	34.524931372690915	36.0	36.0	36.0	32.0	36.0
12	34.36909250334091	36.0	36.0	36.0	32.0	36.0
13	34.46241418571105	36.0	36.0	36.0	32.0	36.0
14	34.391857713516565	36.0	36.0	36.0	32.0	36.0
15	34.33129320867044	36.0	36.0	36.0	32.0	36.0
16	34.32560944427559	36.0	36.0	36.0	32.0	36.0
17	34.269982728592865	36.0	36.0	36.0	32.0	36.0
18	34.28036547358874	36.0	36.0	36.0	32.0	36.0
19	34.26433092595005	36.0	36.0	36.0	32.0	36.0
20	34.25186704876037	36.0	36.0	36.0	32.0	36.0
21	34.21579127649207	36.0	36.0	36.0	32.0	36.0
22	34.173589702767764	36.0	36.0	36.0	32.0	36.0
23	34.15242016798614	36.0	36.0	36.0	32.0	36.0
24	34.11877529802826	36.0	36.0	36.0	32.0	36.0
25	33.63748196874744	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	20.0
4	38.0
5	158.0
6	281.0
7	75.0
8	225.0
9	312.0
10	206.0
11	51.0
12	115.0
13	94.0
14	233.0
15	337.0
16	643.0
17	854.0
18	1177.0
19	1521.0
20	2093.0
21	2749.0
22	3869.0
23	5296.0
24	7402.0
25	10330.0
26	14909.0
27	19535.0
28	27258.0
29	37949.0
30	51098.0
31	73628.0
32	110751.0
33	168488.0
34	401466.0
35	715070.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.124599760323605	17.62821691980249	11.087656786297796	26.159526533576106
2	17.112560367827626	20.031203110903117	36.6620270081198	26.194209513149453
3	18.91582853431322	23.80712009943606	28.50349576124249	28.773555605008227
4	12.68557063535815	15.319295485990903	36.04572754786808	35.949406330782864
5	14.783286449664553	36.255745335713414	33.47596505653489	15.485003158087146
6	33.92674528816991	35.52712948656301	16.890309790120767	13.655815435146309
7	30.027245654423723	30.51935708703825	20.96473151112305	18.48866574741498
8	28.149124151825756	32.2701554930788	19.796124322034	19.784596033061444
9	27.64442985008318	14.060466531293233	18.731936459799645	39.563167158823944
10	15.939425810685734	26.228247225866596	31.49184046446169	26.34048649898598
11	37.45417419361071	21.02184906826898	22.410509867944395	19.113466870175913
12	24.638638574566023	23.782675303391425	28.61471628818094	22.963969833861604
13	29.236742942258658	19.38183743692758	25.427853964815295	25.953565655998467
14	23.556778186819855	19.903717787936408	24.833818689231137	31.7056853360126
15	25.448509275671704	26.992301264930184	22.291255125647798	25.267934333750308
16	25.999789905566175	25.60278387199212	23.70112738605094	24.69629883639077
17	24.042567322787104	25.555246950851345	25.309684820673834	25.09250090568772
18	24.94209356809893	24.49743979904838	26.34682269400768	24.21364393884501
19	25.574295336014778	24.977099439457078	25.380523684644334	24.06808153988381
20	25.639076544280265	24.177528004409943	25.002460366959962	25.18093508434983
21	26.880577061724008	23.940139386539816	24.321634863300474	24.8576486884357
22	25.815534303082277	24.389055144027953	24.800529063780573	24.994881489109197
23	24.350442700187322	24.100680324691087	25.409396524341567	26.139480450780024
24	24.6934297225228	25.102773805843253	25.056519554799817	25.147276916834134
25	24.79155898421838	24.424371899349318	25.30149072763546	25.48257838879684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	149.0
1	149.0
2	456.0
3	763.0
4	763.0
5	763.0
6	2191.5
7	3620.0
8	3620.0
9	3620.0
10	3760.5
11	3901.0
12	3901.0
13	3901.0
14	4454.0
15	5007.0
16	5007.0
17	5007.0
18	8163.0
19	11319.0
20	11319.0
21	11319.0
22	18473.5
23	25628.0
24	25628.0
25	25628.0
26	38514.5
27	51401.0
28	51401.0
29	51401.0
30	62163.5
31	72926.0
32	72926.0
33	72926.0
34	89673.0
35	106420.0
36	106420.0
37	106420.0
38	118886.5
39	131353.0
40	131353.0
41	131353.0
42	148999.5
43	166646.0
44	166646.0
45	166646.0
46	191248.5
47	215851.0
48	215851.0
49	215851.0
50	216050.0
51	216249.0
52	216249.0
53	216249.0
54	200091.5
55	183934.0
56	183934.0
57	183934.0
58	169459.0
59	154984.0
60	154984.0
61	154984.0
62	139558.0
63	124132.0
64	124132.0
65	124132.0
66	104035.5
67	83939.0
68	83939.0
69	83939.0
70	64330.0
71	44721.0
72	44721.0
73	44721.0
74	35512.0
75	26303.0
76	26303.0
77	26303.0
78	21478.0
79	16653.0
80	16653.0
81	16653.0
82	11855.5
83	7058.0
84	7058.0
85	7058.0
86	5197.5
87	3337.0
88	3337.0
89	3337.0
90	2211.5
91	1086.0
92	1086.0
93	1086.0
94	626.5
95	167.0
96	167.0
97	167.0
98	426.0
99	685.0
100	685.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009829746380482346
2	0.0038595323211709823
3	0.0048847205939820245
4	0.014352635819354588
5	0.035157927238166915
6	0.04553042035131393
7	0.0655517442673884
8	0.0867188668413105
9	0.09528220417890862
10	0.11753482021816006
11	0.11819817733586131
12	0.1328523391178074
13	0.12067069022911149
14	0.12416839139517269
15	0.11192643731395847
16	0.11059972307855595
17	0.12254015119717869
18	0.1273645665986424
19	0.13019891064700234
20	0.11910275522363577
21	0.1277263977537522
22	0.14937596186782065
23	0.1361691247063137
24	0.130741657379667
25	0.12832944967893514
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1658232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.90890269984913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2485368154279	37.48801536695897
2	12.82957277464767	12.29301507482457
3	3.7455874254762973	5.383409505627669
4	1.5426854508815657	2.956334686510313
5	0.8042130224760163	1.9264481721877522
6	0.5323174809952982	1.5301647841459516
7	0.3551210913364178	1.1909423268050472
8	0.2687738310087645	1.0301327454451674
9	0.21214928220538992	0.9147455387118774
>10	1.2024511001710227	10.708660385643917
>50	0.11993937613213647	4.016446774402657
>100	0.11834038313155588	12.03578859392186
>500	0.01501319234218595	4.801913660879265
>1k	0.0052987737678303354	3.7239823839349144
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3443	0.2076307778404952	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2288	0.13797828048186261	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1914	0.11542413848001967	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1903	0.11476078136231842	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1886	0.11373559308950737	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1858	0.11204704769899507	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1851	0.111624911351367	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1832	0.11047911269351936	No Hit
GTACATGGAAGCAGTGGTATCAACG	1798	0.10842873614789728	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1792	0.1080669049927875	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1784	0.10758446345264112	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1698	0.10239821689606762	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2061038503659318E-4	0.0
5	0.0	0.0	0.0	1.2061038503659318E-4	0.0
6	0.0	0.0	0.0	1.2061038503659318E-4	0.0
7	0.0	0.0	0.0	1.2061038503659318E-4	0.0
8	0.0	0.0	0.0	1.2061038503659318E-4	0.0
9	0.0	0.0	0.0	1.2061038503659318E-4	0.0
10	0.0	0.0	0.0	3.01525962591483E-4	0.0
11	0.0	0.0	0.0	3.01525962591483E-4	0.0
12	0.0	0.0	0.0	3.01525962591483E-4	0.0
13	0.0	0.0	0.0	3.01525962591483E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGC	40	0.0052826367	14.248641	4
GTATTAC	90	3.6670826E-8	13.714287	1
AATTTCG	70	7.2477415E-6	13.574234	18
CGAACGA	180	0.0	13.197172	16
ATTTCGG	60	4.0851478E-4	12.669285	19
AAAGCGT	75	1.4769577E-5	12.668138	10
GTACTAA	70	1.0968902E-4	12.207224	1
ATCGCCA	355	0.0	12.044742	16
TAAGACC	95	1.0410968E-6	11.998856	4
CGCCAGT	360	0.0	11.877455	18
GATAACG	200	0.0	11.875661	11
CGGAATT	120	1.0009899E-8	11.875302	15
CGCATCG	355	0.0	11.775303	13
GCATCGC	355	0.0	11.774947	14
CTTACAC	130	2.6284397E-9	11.688369	3
CGATAAC	205	0.0	11.586711	10
ATAACGA	205	0.0	11.585661	12
CGGTCCA	430	0.0	11.268749	10
CAACGGA	110	4.966696E-7	11.227558	14
GTTTTCG	220	0.0	11.227558	15
>>END_MODULE