FastQCFastQC Report
Thu 2 Feb 2017
SRR4062404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658232
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18860.11373559308950737No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18030.10873026211048877No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17980.10842873614789728No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG17710.10680049594990328No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA17200.10372493113147015No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17100.10312187920628718No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16920.10203638574095784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG755.474467E-520.5332075
GTGCGAA951.5977785E-518.52620111
TAAGTCG600.007413498618.3332215
TATGCCG600.007413498618.33322129
TACCGTA600.007413498618.3332217
CGCAATA5100.018.11753536
CGTCGTA5000.018.03988810
ACGGGTC750.001291198217.5998929
TTAACGG2650.017.43385535
GTATCAA36250.017.3607981
ATACCGT5450.017.357696
CGTCTAT1154.2664797E-617.220921
TACCGTC5900.017.1524377
GTCGTAG5350.016.85970911
ATTGTCG2750.016.79989643
CTCGACG1451.2501368E-716.68955213
TAACGGC2650.016.60367236
TAGATCG800.001988852616.4998995
ATACGAA5650.016.35388240
ACCGTCG5900.016.03388
Produced by FastQC (version 0.11.5)