##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062404_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1658232
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240671389769346	32.0	32.0	32.0	32.0	32.0
2	31.302290632432616	32.0	32.0	32.0	32.0	32.0
3	31.428685491535564	32.0	32.0	32.0	32.0	32.0
4	31.557549848272135	32.0	32.0	32.0	32.0	32.0
5	31.459515918158615	32.0	32.0	32.0	32.0	32.0
6	35.05523654108713	36.0	36.0	36.0	36.0	36.0
7	35.0735168540952	36.0	36.0	36.0	36.0	36.0
8	35.00731260764477	36.0	36.0	36.0	36.0	36.0
9	35.153597928396024	36.0	36.0	36.0	36.0	36.0
10	35.002479749516354	36.0	36.0	36.0	36.0	36.0
11	35.171056884681995	36.0	36.0	36.0	36.0	36.0
12	35.0658201023741	36.0	36.0	36.0	36.0	36.0
13	35.11816923084345	36.0	36.0	36.0	36.0	36.0
14	35.07286254275638	36.0	36.0	36.0	36.0	36.0
15	35.02223030311802	36.0	36.0	36.0	36.0	36.0
16	35.036778327761134	36.0	36.0	36.0	36.0	36.0
17	35.0084988107816	36.0	36.0	36.0	36.0	36.0
18	35.027599274407926	36.0	36.0	36.0	36.0	36.0
19	35.0067529754582	36.0	36.0	36.0	36.0	36.0
20	34.994806516820326	36.0	36.0	36.0	36.0	36.0
21	34.9941618543123	36.0	36.0	36.0	36.0	36.0
22	34.967638424538904	36.0	36.0	36.0	32.0	36.0
23	34.938773344140024	36.0	36.0	36.0	32.0	36.0
24	34.9062290439456	36.0	36.0	36.0	32.0	36.0
25	34.881461701378335	36.0	36.0	36.0	32.0	36.0
26	34.83476377249987	36.0	36.0	36.0	32.0	36.0
27	34.81666919948475	36.0	36.0	36.0	32.0	36.0
28	34.79543634425099	36.0	36.0	36.0	32.0	36.0
29	34.777777174725856	36.0	36.0	36.0	32.0	36.0
30	34.761701016504325	36.0	36.0	36.0	32.0	36.0
31	34.765205954293485	36.0	36.0	36.0	32.0	36.0
32	34.725868877213806	36.0	36.0	36.0	32.0	36.0
33	34.70426212978642	36.0	36.0	36.0	32.0	36.0
34	34.6904992787499	36.0	36.0	36.0	32.0	36.0
35	34.66824002913947	36.0	36.0	36.0	32.0	36.0
36	34.63783656327944	36.0	36.0	36.0	32.0	36.0
37	34.63203761596689	36.0	36.0	36.0	32.0	36.0
38	34.59784577791286	36.0	36.0	36.0	32.0	36.0
39	34.58129079646274	36.0	36.0	36.0	32.0	36.0
40	34.58229306876239	36.0	36.0	36.0	32.0	36.0
41	34.54427607234693	36.0	36.0	36.0	32.0	36.0
42	34.51340584429682	36.0	36.0	36.0	32.0	36.0
43	34.50975798320138	36.0	36.0	36.0	32.0	36.0
44	34.47470559005013	36.0	36.0	36.0	32.0	36.0
45	34.428142141750975	36.0	36.0	36.0	32.0	36.0
46	34.44504025974653	36.0	36.0	36.0	32.0	36.0
47	34.41422310026582	36.0	36.0	36.0	32.0	36.0
48	34.383587459414606	36.0	36.0	36.0	32.0	36.0
49	34.37388556004226	36.0	36.0	36.0	32.0	36.0
50	33.8020771520511	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	1.0
20	12.0
21	35.0
22	113.0
23	370.0
24	1019.0
25	2322.0
26	5194.0
27	9515.0
28	16298.0
29	25771.0
30	38170.0
31	56626.0
32	85962.0
33	145603.0
34	320757.0
35	950461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35226158991324	18.26973821105358	11.652488065548262	26.72551213348492
2	16.491805750297768	20.61477226468859	36.304913534458066	26.58850845055558
3	18.32252757623984	24.186764007933803	28.83505877684579	28.655649638980567
4	12.381108026158042	15.886717628697621	36.22716538377111	35.50500896137322
5	14.635708393035474	36.46648961062143	33.287561692211945	15.610240304131148
6	34.11676234137387	35.83794218268592	16.60397907880906	13.441316397131144
7	30.19900737533394	30.51983138647835	20.810804291322675	18.47035694686503
8	28.269385707186935	32.425619575547934	19.571989926620642	19.733004790644493
9	27.71433671524853	13.902216336435433	18.57550692544831	39.807940022867726
10	15.940290622783785	26.307959320529335	31.385716835762427	26.366033220924457
11	37.798691618543124	20.843464605676406	22.284517486093623	19.073326289686847
12	24.7040221151202	23.639514856787226	28.528818645400644	23.12764438269193
13	29.369231808335627	19.277579976746317	25.393913517529516	25.959274697388544
14	23.57643562541309	19.81134123572576	24.75932197665948	31.85290116220167
15	25.572597802961226	27.003459105842847	22.02653187250035	25.397411218695577
16	26.191992435316653	25.626872476227692	23.387620067638302	24.793515020817352
17	24.2175401270751	25.655758663443955	25.058254815972674	25.068446393508264
18	25.24707037374746	24.516231745618224	26.10243922442698	24.134258656207333
19	25.815507118424925	25.0524052122984	25.000663357117702	24.131424312158973
20	25.867248973605623	24.246788145446477	24.661687869972358	25.224275010975543
21	27.105555796776326	24.023538322743743	23.91872789814694	24.95217798233299
22	25.951917463901314	24.485476097433896	24.475766961438445	25.08683947722635
23	24.59034682722321	24.024081069476406	25.10064936631304	26.284922736987344
24	24.78296161212665	25.25171387357137	24.718495361324592	25.24682915297739
25	25.07188378948181	24.406174769272333	24.967555806425157	25.554385634820704
26	24.60922235248144	25.233501705430843	25.430639379773158	24.72663656231456
27	25.651718215545234	24.790620371576473	24.460570052923835	25.09709135995446
28	24.480108935299764	24.627736046584555	25.445052320785027	25.447102697330653
29	24.641907766826353	24.846824811003525	25.205459790909835	25.30580763126028
30	24.70378089435013	25.007236623102198	25.45331413216004	24.835668350387643
31	25.159386623825856	24.754015119717867	24.451765494816165	25.63483276164011
32	24.818119539364815	24.888917835381296	24.514422589842678	25.778540035411208
33	24.62731391023693	24.354734440054226	25.102337911703547	25.915613738005298
34	25.135746988358687	24.616157449621042	25.43425769132425	24.813837870696016
35	25.987979969027254	24.47468146797312	24.915150594126757	24.622187968872872
36	24.738637295625704	25.035519758393278	24.768669281499815	25.457173664481207
37	25.8694802657288	24.82903477921063	24.422035034904646	24.879449920155924
38	24.801234085459694	24.622429189642943	25.102398216896066	25.473938508001293
39	25.257081035705497	24.565561393098193	24.51617144042571	25.661186130770602
40	25.58055808837364	24.687498492370185	25.19924835608045	24.53269506317572
41	24.43252813840283	24.931131470144106	25.72547146599511	24.910868925457958
42	25.763584347666672	25.166804162505606	24.937825346513637	24.131786143314084
43	24.71137934860743	24.164773083621593	25.288138209852423	25.835709357918553
44	24.505618031735004	24.721209094987913	25.104207372671617	25.668965500605463
45	24.641485630478726	24.945604716348495	25.175367499843205	25.237542153329574
46	24.545675481884007	24.662727931150727	25.279409201733653	25.51218738523161
47	24.686835135252487	24.33911539519199	25.73029588139657	25.243753588158956
48	25.358393759136238	25.240255886992895	24.661627564779838	24.739722789091033
49	24.31927498685347	25.09799593784223	25.259191717443642	25.32353735786066
50	24.09186410586697	25.511810168902784	24.915934561629495	25.480391163600753
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	12.0
2	11.0
3	57.0
4	103.0
5	118.0
6	133.0
7	141.0
8	149.0
9	213.0
10	277.0
11	401.0
12	525.0
13	944.0
14	1363.0
15	1803.0
16	2243.0
17	2910.0
18	3577.0
19	4112.5
20	4648.0
21	5812.5
22	6977.0
23	7601.5
24	8226.0
25	10047.5
26	11869.0
27	15124.5
28	18380.0
29	21146.5
30	23913.0
31	25185.5
32	26458.0
33	30673.0
34	34888.0
35	40274.5
36	45661.0
37	55421.5
38	65182.0
39	69730.5
40	74279.0
41	80683.0
42	87087.0
43	87472.5
44	87858.0
45	96343.5
46	104829.0
47	111615.5
48	118402.0
49	121196.0
50	123990.0
51	119345.5
52	114701.0
53	111934.5
54	109168.0
55	111341.0
56	113514.0
57	111425.5
58	109337.0
59	100457.5
60	91578.0
61	81061.0
62	70544.0
63	62547.0
64	54550.0
65	47485.5
66	40421.0
67	35619.5
68	30818.0
69	28403.5
70	25989.0
71	20549.5
72	15110.0
73	13638.5
74	12167.0
75	8982.0
76	5797.0
77	5261.0
78	4725.0
79	4094.0
80	3463.0
81	2757.0
82	2051.0
83	1799.0
84	1547.0
85	1273.5
86	1000.0
87	749.5
88	499.0
89	314.5
90	130.0
91	89.0
92	48.0
93	32.0
94	16.0
95	13.0
96	10.0
97	8.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013086226776470361
2	0.003437395973542906
3	6.633571177012625E-4
4	2.412207700731864E-4
5	0.0
6	5.427467326646694E-4
7	1.206103850365932E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.03051925182966E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1658232.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.7856993234318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17580368740641	41.001743634470905
2	12.594153087087633	13.043940500023812
3	3.547340828038106	5.511045765555447
4	1.39100422566066	2.8813650635074404
5	0.7728239227146498	2.0010613645827973
6	0.47641797306356504	1.4802982743209163
7	0.3317985386138572	1.2027693549627898
8	0.23866610870977517	0.9887593075470322
9	0.18856109758840092	0.878829147342829
>10	1.071715790104446	10.231253663493602
>50	0.09949898735254308	3.6037810895379905
>100	0.09677022796598125	10.591114943393638
>500	0.01193517894527407	4.121264993174171
>1k	0.0035103467486100205	2.4627728980868047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1886	0.11373559308950737	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1803	0.10873026211048877	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1798	0.10842873614789728	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1771	0.10680049594990328	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1720	0.10372493113147015	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1710	0.10312187920628718	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1692	0.10203638574095784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.030519251829659E-5
2	0.0	0.0	0.0	6.030519251829659E-5	6.030519251829659E-5
3	0.0	0.0	0.0	6.030519251829659E-5	6.030519251829659E-5
4	0.0	0.0	0.0	1.2061038503659318E-4	6.030519251829659E-5
5	0.0	0.0	0.0	1.2061038503659318E-4	6.030519251829659E-5
6	0.0	0.0	0.0	1.2061038503659318E-4	6.030519251829659E-5
7	0.0	0.0	0.0	1.809155775548898E-4	6.030519251829659E-5
8	0.0	0.0	0.0	1.809155775548898E-4	6.030519251829659E-5
9	0.0	0.0	0.0	1.809155775548898E-4	6.030519251829659E-5
10	0.0	0.0	0.0	1.809155775548898E-4	6.030519251829659E-5
11	0.0	0.0	0.0	1.809155775548898E-4	6.030519251829659E-5
12	0.0	0.0	0.0	1.809155775548898E-4	6.030519251829659E-5
13	0.0	0.0	0.0	1.809155775548898E-4	6.030519251829659E-5
14	0.0	0.0	0.0	1.809155775548898E-4	6.030519251829659E-5
15	0.0	0.0	0.0	1.809155775548898E-4	6.030519251829659E-5
16	0.0	0.0	0.0	1.809155775548898E-4	1.2061038503659318E-4
17	0.0	0.0	0.0	2.4122077007318637E-4	1.2061038503659318E-4
18	0.0	0.0	0.0	4.2213634762807616E-4	1.809155775548898E-4
19	0.0	0.0	0.0	4.8244154014637274E-4	1.809155775548898E-4
20	0.0	0.0	0.0	6.03051925182966E-4	2.4122077007318637E-4
21	0.0	0.0	0.0	7.839675027378558E-4	2.4122077007318637E-4
22	0.0	0.0	0.0	9.648830802927455E-4	2.4122077007318637E-4
23	0.0	0.0	0.0	0.0015679350054757116	2.4122077007318637E-4
24	0.0	0.0	0.0	0.002351902508213567	2.4122077007318637E-4
25	0.0	0.0	0.0	0.002954954433396533	2.4122077007318637E-4
26	0.0	0.0	0.0	0.004221363476280762	3.01525962591483E-4
27	0.0	0.0	0.0	0.005125941364055211	3.618311551097796E-4
28	0.0	0.0	0.0	0.009950356765518939	3.618311551097796E-4
29	0.0	0.0	0.0	0.020443460263702546	3.618311551097796E-4
30	0.0	0.0	0.0	0.03853501801919153	3.618311551097796E-4
31	0.0	0.0	0.0	0.08653795126375562	4.2213634762807616E-4
32	0.0	0.0	0.0	0.141717202417997	4.2213634762807616E-4
33	0.0	0.0	0.0	0.19924835608045194	4.2213634762807616E-4
34	0.0	0.0	0.0	0.26642834054583436	4.2213634762807616E-4
35	0.0	0.0	0.0	0.34114647407600385	4.2213634762807616E-4
36	0.0	0.0	0.0	0.450781314074267	4.2213634762807616E-4
37	0.0	0.0	0.0	0.6116152625205641	4.2213634762807616E-4
38	0.0	0.0	0.0	0.8136376574568577	4.2213634762807616E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	75	5.474467E-5	20.533207	5
GTGCGAA	95	1.5977785E-5	18.526201	11
TAAGTCG	60	0.0074134986	18.333221	5
TATGCCG	60	0.0074134986	18.333221	29
TACCGTA	60	0.0074134986	18.333221	7
CGCAATA	510	0.0	18.117535	36
CGTCGTA	500	0.0	18.039888	10
ACGGGTC	75	0.0012911982	17.599892	9
TTAACGG	265	0.0	17.433855	35
GTATCAA	3625	0.0	17.360798	1
ATACCGT	545	0.0	17.35769	6
CGTCTAT	115	4.2664797E-6	17.22092	1
TACCGTC	590	0.0	17.152437	7
GTCGTAG	535	0.0	16.859709	11
ATTGTCG	275	0.0	16.799896	43
CTCGACG	145	1.2501368E-7	16.689552	13
TAACGGC	265	0.0	16.603672	36
TAGATCG	80	0.0019888526	16.499899	5
ATACGAA	565	0.0	16.353882	40
ACCGTCG	590	0.0	16.0338	8
>>END_MODULE