FastQCFastQC Report
Thu 2 Feb 2017
SRR4062403_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062403_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436243
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG49240.3428389207118851No Hit
TCCATGTACTCTGCGTTGATACCAC41470.2887394403314759No Hit
GTATCAACGCAGAGTACTTTTTTTT39700.2764156204764793No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31350.21827782624528025No Hit
TATCAACGCAGAGTACTTTTTTTTT28150.19599747396506023No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21980.15303816972476106No Hit
GAGTACATGGAAGCAGTGGTATCAA19250.13403024418569837No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16950.11801624098429027No Hit
CATGTACTCTGCGTTGATACCACTG16260.11321204002386782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGT1500.015.201069
AATCGTT400.00528006214.2495035
CGTCGTA1500.013.93430510
CGGCTAA500.001498661813.3009279
AACGGAC1001.4408033E-712.35086215
CGTCTTA1251.4078978E-912.16084815
ACCGTCG1800.012.1397358
TCCAACG1650.012.09470418
CGAACGT550.003069849512.0900674
GTGATAC550.003075464412.0871173
AACCGCG802.8639555E-511.8758287
GAGCGTT802.8639555E-511.8758287
ACTGTTC3850.011.5982118
GTCGGCG1157.0638634E-811.56602412
TCGGCGT1157.0673195E-811.5656213
AAACCGA752.0689069E-411.40278216
GCATCGC1501.7644197E-1011.40079514
TCGGCAA752.0729311E-411.40039713
GTCGTAG1850.011.29769111
ATAATAC1354.7475623E-911.2557283
Produced by FastQC (version 0.11.5)