FastQCFastQC Report
Thu 2 Feb 2017
SRR4062403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436243
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23190.16146292793071923No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18790.13082744354541676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG858.236384E-925.8829885
ATACCGA752.0642783E-623.4672416
CGGCGTT2150.020.46490541
CGCCGGT3650.019.2874747
CTAGCGG3700.019.0268329
CCGCGCA708.124902E-418.8569499
TAGCGGC3900.018.61519230
CGCAATA3550.018.59135836
GGCGTTA2400.018.33314542
GACCGTA600.00741320318.3331457
CAATACG3850.018.28552638
TAGGACG6850.017.9858444
CGACCAT2450.017.95910
CTTGCGC4300.017.9074153
TCTAGCG3950.017.82260128
CGTTATT2500.017.5998244
ATACGAA4000.017.5998240
CGGTCCA3900.017.48710
CCGACCA2650.017.4337839
TACGACG2800.017.2861395
Produced by FastQC (version 0.11.5)