FastQCFastQC Report
Thu 2 Feb 2017
SRR4062402_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062402_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775178
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG44570.5749647177809484No Hit
TCCATGTACTCTGCGTTGATACCAC38280.49382206409366625No Hit
GTATCAACGCAGAGTACTTTTTTTT31700.4089383341632502No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22240.28690184705964306No Hit
TATCAACGCAGAGTACTTTTTTTTT20490.26432638697176647No Hit
GAGTACATGGAAGCAGTGGTATCAA18720.24149292162574273No Hit
CATGTACTCTGCGTTGATACCACTG18250.23542979805928443No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17070.22020748782860194No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12980.1674454125375075No Hit
CCCATGTACTCTGCGTTGATACCAC11520.14861102869276477No Hit
GCGTTGATACCACTGCTTCCATGTA11400.1470629971438818No Hit
GCTTCCATGTACTCTGCGTTGATAC10940.14112887620649708No Hit
GTACATGGGAAGCAGTGGTATCAAC10740.1385488236250255No Hit
GGTATCAACGCAGAGTACTTTTTTT10630.13712979470521608No Hit
CATGGAAGCAGTGGTATCAACGCAG9920.1279706080409919No Hit
GTACATGGGGTGGTATCAACGCAAA8630.1113292688905No Hit
GCGCAAGACGGACCAGAGCGAAAGC8460.10913622419624913No Hit
GTATCAACGCAGAGTACATGGAAGC8370.1079752005345869No Hit
GTCTTGCGCCGGTCCAAGAATTTCA8320.107330187389219No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG350.002180828216.2723221
GCCGGTT551.1266671E-515.54571911
GCATCGC1250.015.20320514
CGGTCCA1500.015.20025910
CGCATCG1200.015.04483913
ATCCCCG1151.8189894E-1214.87173912
CATAGGA456.7892077E-414.7684862
TCTCGTC400.00527081714.25208412
CTATTTG400.005304810614.2382821
CCGCATC1301.8189894E-1213.88664612
TAGACAA551.9589427E-413.8175235
CGCCAGT1450.013.76330218
GAACAGT1650.013.2426496
CGCCGGT1750.013.0287927
AATCCCC1251.0186341E-1012.9202211
TTCGGAA1403.6379788E-1212.897243518
TTACCGC1403.6379788E-1212.89557616
ACAGTCC1207.366907E-1012.66688258
TAATGTG604.0895445E-412.6660635
TAATCCC751.47703595E-512.6660635
Produced by FastQC (version 0.11.5)