##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062402_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775178
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09809488917384	32.0	32.0	32.0	32.0	32.0
2	30.88449620603268	32.0	32.0	32.0	32.0	32.0
3	30.87553568341723	32.0	32.0	32.0	32.0	32.0
4	30.886695700858382	32.0	32.0	32.0	32.0	32.0
5	30.78595109768337	32.0	32.0	32.0	32.0	32.0
6	34.39505894130122	36.0	36.0	36.0	32.0	36.0
7	34.24987809251552	36.0	36.0	36.0	32.0	36.0
8	34.25369270025723	36.0	36.0	36.0	32.0	36.0
9	34.34318569412444	36.0	36.0	36.0	32.0	36.0
10	34.125698871743005	36.0	36.0	36.0	32.0	36.0
11	34.37784096039877	36.0	36.0	36.0	32.0	36.0
12	34.19348717326859	36.0	36.0	36.0	32.0	36.0
13	34.29214709395777	36.0	36.0	36.0	32.0	36.0
14	34.2171307751252	36.0	36.0	36.0	32.0	36.0
15	34.187302787230806	36.0	36.0	36.0	32.0	36.0
16	34.188503801707476	36.0	36.0	36.0	32.0	36.0
17	34.106044031177355	36.0	36.0	36.0	32.0	36.0
18	34.129129051650075	36.0	36.0	36.0	32.0	36.0
19	34.108994321304266	36.0	36.0	36.0	32.0	36.0
20	34.07124815203734	36.0	36.0	36.0	32.0	36.0
21	34.02529999561391	36.0	36.0	36.0	32.0	36.0
22	34.00127067589637	36.0	36.0	36.0	32.0	36.0
23	33.96536408412003	36.0	36.0	36.0	32.0	36.0
24	33.94945934998155	36.0	36.0	36.0	32.0	36.0
25	33.47278689539693	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	25.0
5	65.0
6	144.0
7	27.0
8	113.0
9	149.0
10	103.0
11	32.0
12	63.0
13	36.0
14	178.0
15	224.0
16	331.0
17	484.0
18	680.0
19	994.0
20	1322.0
21	1894.0
22	2615.0
23	3634.0
24	4843.0
25	6388.0
26	8764.0
27	10950.0
28	14937.0
29	19916.0
30	25960.0
31	35235.0
32	50825.0
33	74117.0
34	168829.0
35	341297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.80215997657079	17.28897266291748	11.653320018939468	25.25554734157226
2	15.732309875507966	20.514223053602528	38.11223634135328	25.64123072953622
3	19.759630828455727	24.669060209690883	28.171261744637427	27.400047217215967
4	12.436295418947129	15.664166221540988	35.83068193254217	36.06885642696972
5	14.119704223716303	37.064820430759696	33.474468002735804	15.341007342788195
6	33.553943426933444	34.977498028576925	17.503010372718343	13.965548171771285
7	29.172620960923044	30.937157507058018	21.07297350677918	18.817248025239753
8	27.913955169920463	33.036618117963016	20.231381055676067	18.81804565644045
9	27.1441834999335	14.97541996541879	18.86445018071846	39.01594635392925
10	15.74537719290315	27.294613301233824	31.73330887984512	25.226700626017905
11	36.738294544407175	21.819658939879854	21.924020288466135	19.51802622724684
12	24.186514549085565	24.097512329843	29.512955054525165	22.203018066546274
13	29.85832247298259	19.866627573709383	25.454810823238265	24.82023913006976
14	23.496300812168055	20.11652958316973	25.616229928289485	30.770939676372727
15	25.15239807410163	27.178388403281467	23.79670096914843	23.87251255346848
16	24.90678713630194	26.155393947847287	25.266336305038205	23.671482610812564
17	23.21502840299969	25.902269915036413	26.64043791509841	24.242263766865495
18	24.25828902701781	24.881677594696676	28.1897963717907	22.670237006494816
19	24.60051929262527	26.05996408871895	26.007388939842148	23.332127678813634
20	24.597558030046073	24.529230403597985	27.28003089597022	23.59318067038572
21	25.81394357519024	24.977814749606996	24.908061997279642	24.300179677923126
22	24.425420638991422	25.43691804015757	25.826064824205652	24.31159649664535
23	23.342100707676963	25.07382938758426	26.063905374366026	25.520164530372753
24	23.621942476247067	26.31146535075538	26.135910039206063	23.930682133791493
25	24.462830200865465	25.091261383452817	25.890072983271978	24.55583543240974
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	136.0
1	136.0
2	401.0
3	666.0
4	666.0
5	666.0
6	1861.5
7	3057.0
8	3057.0
9	3057.0
10	3106.0
11	3155.0
12	3155.0
13	3155.0
14	2824.0
15	2493.0
16	2493.0
17	2493.0
18	4178.0
19	5863.0
20	5863.0
21	5863.0
22	9536.0
23	13209.0
24	13209.0
25	13209.0
26	19550.5
27	25892.0
28	25892.0
29	25892.0
30	33343.0
31	40794.0
32	40794.0
33	40794.0
34	46396.0
35	51998.0
36	51998.0
37	51998.0
38	57284.5
39	62571.0
40	62571.0
41	62571.0
42	71998.0
43	81425.0
44	81425.0
45	81425.0
46	98397.5
47	115370.0
48	115370.0
49	115370.0
50	108389.5
51	101409.0
52	101409.0
53	101409.0
54	90477.0
55	79545.0
56	79545.0
57	79545.0
58	72820.5
59	66096.0
60	66096.0
61	66096.0
62	58252.5
63	50409.0
64	50409.0
65	50409.0
66	41736.5
67	33064.0
68	33064.0
69	33064.0
70	25162.5
71	17261.0
72	17261.0
73	17261.0
74	13520.0
75	9779.0
76	9779.0
77	9779.0
78	8095.0
79	6411.0
80	6411.0
81	6411.0
82	4490.0
83	2569.0
84	2569.0
85	2569.0
86	1889.0
87	1209.0
88	1209.0
89	1209.0
90	804.5
91	400.0
92	400.0
93	400.0
94	243.0
95	86.0
96	86.0
97	86.0
98	198.5
99	311.0
100	311.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0099332024386657
2	0.0036120736140602544
3	0.004773097275722479
4	0.01496430497253534
5	0.03457270459171958
6	0.04605393857926825
7	0.06798438552177694
8	0.0900438350933592
9	0.09920302175758342
10	0.1185534161186205
11	0.12190748447453359
12	0.13416273423652375
13	0.1231975107652694
14	0.12306850813619583
15	0.11481233987548665
16	0.11287730043938295
17	0.12435853442693161
18	0.1318406869131993
19	0.13519475526911237
20	0.12474554231415236
21	0.13093766850968422
22	0.15183609441960427
23	0.14138688146464426
24	0.13712979470521608
25	0.13261470268764078
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	775178.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.779203026523064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60187867113707	44.15306676045396
2	12.326407782380276	13.504615889974461
3	3.329989728853027	5.4724255029922935
4	1.2826108343642928	2.810415971986389
5	0.6197685476652862	1.6975213551005022
6	0.36016263259958997	1.1837653184243984
7	0.25237332581710914	0.9677366757389992
8	0.15955504637802043	0.6992238623558306
9	0.11854591099277373	0.5844465473613555
>10	0.738826509032322	7.8710525693403355
>50	0.09962875470776518	3.851861173629776
>100	0.0996283770082187	10.919005966584129
>500	0.007318672244292275	2.669808825866877
>1k	0.003305206820002963	3.6150535801906996
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4457	0.5749647177809484	No Hit
TCCATGTACTCTGCGTTGATACCAC	3828	0.49382206409366625	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3170	0.4089383341632502	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2224	0.28690184705964306	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2049	0.26432638697176647	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1872	0.24149292162574273	No Hit
CATGTACTCTGCGTTGATACCACTG	1825	0.23542979805928443	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1707	0.22020748782860194	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1298	0.1674454125375075	No Hit
CCCATGTACTCTGCGTTGATACCAC	1152	0.14861102869276477	No Hit
GCGTTGATACCACTGCTTCCATGTA	1140	0.1470629971438818	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1094	0.14112887620649708	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1074	0.1385488236250255	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1063	0.13712979470521608	No Hit
CATGGAAGCAGTGGTATCAACGCAG	992	0.1279706080409919	No Hit
GTACATGGGGTGGTATCAACGCAAA	863	0.1113292688905	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	846	0.10913622419624913	No Hit
GTATCAACGCAGAGTACATGGAAGC	837	0.1079752005345869	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	832	0.107330187389219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.2900262907358052E-4
13	0.0	0.0	0.0	0.0	1.2900262907358052E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTTAG	35	0.0021808282	16.272322	1
GCCGGTT	55	1.1266671E-5	15.545719	11
GCATCGC	125	0.0	15.203205	14
CGGTCCA	150	0.0	15.200259	10
CGCATCG	120	0.0	15.044839	13
ATCCCCG	115	1.8189894E-12	14.871739	12
CATAGGA	45	6.7892077E-4	14.768486	2
TCTCGTC	40	0.005270817	14.252084	12
CTATTTG	40	0.0053048106	14.238282	1
CCGCATC	130	1.8189894E-12	13.886646	12
TAGACAA	55	1.9589427E-4	13.817523	5
CGCCAGT	145	0.0	13.763302	18
GAACAGT	165	0.0	13.242649	6
CGCCGGT	175	0.0	13.028792	7
AATCCCC	125	1.0186341E-10	12.92022	11
TTCGGAA	140	3.6379788E-12	12.8972435	18
TTACCGC	140	3.6379788E-12	12.895576	16
ACAGTCC	120	7.366907E-10	12.6668825	8
TAATGTG	60	4.0895445E-4	12.666063	5
TAATCCC	75	1.47703595E-5	12.666063	5
>>END_MODULE