FastQCFastQC Report
Thu 2 Feb 2017
SRR4062402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775178
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20730.2674224500695324No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18490.23852586115705038No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8930.1151993477627074No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA8920.11507034513363382No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8850.11416732673011877No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8380.10810420316366047No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8210.1059111584694096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA609.742875E-625.6680972
ATACCTA752.0628358E-623.4664616
GATATAC4350.022.7657621
ACTGTTC4650.022.2363628
GTCCAAT602.8654028E-422.0069031
GCCGGTT1700.021.99980721
ATGCCGG1800.020.77759619
AACGAAT2650.020.75453431
ATACACT4900.020.652884
CCGGCAT550.004482412719.99982644
CGTCTTA550.004482412719.99982615
GGGCGGA550.004482412719.99982620
CACTGTT5500.019.9998257
AACGTAT1452.9467628E-1019.72396531
CGAATGT2600.019.46136733
TGGTATA2050.019.31690244
TCCAACG4000.019.24983228
CCGTCCA808.98388E-519.249839
TAGGACG2650.019.0941724
TATGCGT708.1202114E-418.85697739
Produced by FastQC (version 0.11.5)