##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062402_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775178
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.191523753254092	32.0	32.0	32.0	32.0	32.0
2	31.415166581094923	32.0	32.0	32.0	32.0	32.0
3	31.503256026357818	32.0	32.0	32.0	32.0	32.0
4	31.60708895247285	32.0	32.0	32.0	32.0	32.0
5	31.546034588184906	32.0	32.0	32.0	32.0	32.0
6	35.150014577297085	36.0	36.0	36.0	36.0	36.0
7	35.15890414846655	36.0	36.0	36.0	36.0	36.0
8	35.09969065169548	36.0	36.0	36.0	36.0	36.0
9	35.20248897672535	36.0	36.0	36.0	36.0	36.0
10	35.09673262141082	36.0	36.0	36.0	36.0	36.0
11	35.21467198501505	36.0	36.0	36.0	36.0	36.0
12	35.11685316146743	36.0	36.0	36.0	36.0	36.0
13	35.15673948435069	36.0	36.0	36.0	36.0	36.0
14	35.133321637094966	36.0	36.0	36.0	36.0	36.0
15	35.10682836716212	36.0	36.0	36.0	36.0	36.0
16	35.105152623010454	36.0	36.0	36.0	36.0	36.0
17	35.08380397792507	36.0	36.0	36.0	36.0	36.0
18	35.09628240223536	36.0	36.0	36.0	36.0	36.0
19	35.064151717412	36.0	36.0	36.0	36.0	36.0
20	35.03647936344943	36.0	36.0	36.0	36.0	36.0
21	35.01496430497254	36.0	36.0	36.0	36.0	36.0
22	35.00244072974207	36.0	36.0	36.0	36.0	36.0
23	34.95652095389704	36.0	36.0	36.0	32.0	36.0
24	34.94510938132919	36.0	36.0	36.0	32.0	36.0
25	34.917378976183535	36.0	36.0	36.0	32.0	36.0
26	34.87434756920346	36.0	36.0	36.0	32.0	36.0
27	34.85204559468922	36.0	36.0	36.0	32.0	36.0
28	34.82934629207743	36.0	36.0	36.0	32.0	36.0
29	34.78982375660816	36.0	36.0	36.0	32.0	36.0
30	34.771759518458985	36.0	36.0	36.0	32.0	36.0
31	34.75853545895265	36.0	36.0	36.0	32.0	36.0
32	34.72086927131575	36.0	36.0	36.0	32.0	36.0
33	34.68208334085848	36.0	36.0	36.0	32.0	36.0
34	34.658831906994266	36.0	36.0	36.0	32.0	36.0
35	34.594312532089404	36.0	36.0	36.0	32.0	36.0
36	34.57335218491753	36.0	36.0	36.0	32.0	36.0
37	34.53051428188107	36.0	36.0	36.0	32.0	36.0
38	34.46934381522696	36.0	36.0	36.0	32.0	36.0
39	34.4166320509612	36.0	36.0	36.0	32.0	36.0
40	34.36938225801042	36.0	36.0	36.0	32.0	36.0
41	34.35190756187611	36.0	36.0	36.0	32.0	36.0
42	34.25372624094079	36.0	36.0	36.0	32.0	36.0
43	34.260785264803694	36.0	36.0	36.0	32.0	36.0
44	34.22346738426529	36.0	36.0	36.0	32.0	36.0
45	34.10201011896623	36.0	36.0	36.0	32.0	36.0
46	34.13473808596219	36.0	36.0	36.0	32.0	36.0
47	34.06241534202467	36.0	36.0	36.0	32.0	36.0
48	34.03320785677612	36.0	36.0	36.0	32.0	36.0
49	34.01665810949227	36.0	36.0	36.0	32.0	36.0
50	33.47027392418258	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	3.0
21	22.0
22	62.0
23	228.0
24	558.0
25	1414.0
26	2954.0
27	5611.0
28	9647.0
29	14622.0
30	20528.0
31	28396.0
32	41128.0
33	61956.0
34	129283.0
35	458763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18920906564412	18.151157719809355	12.210182979854412	25.44945023469211
2	15.339045805564371	20.97450028768774	37.603754647511565	26.082699259236325
3	19.259700737770793	24.84103548498206	28.43790998912501	27.461353788122132
4	12.23425389846951	16.074930080394644	35.866564496320834	35.82425152481501
5	13.924027771685987	37.20913132209635	33.34137449721225	15.525466409005414
6	33.58338439707163	35.317057438642884	17.198310059018933	13.901248105266554
7	29.271986563086156	30.883100397586105	21.044714891289484	18.80019814803826
8	28.003374708776562	33.20243866570001	20.065197928733788	18.728988696789642
9	27.380808020867462	14.578329106347187	18.813743424090983	39.22711944869437
10	15.871709465438904	27.40776957034384	31.633637693536194	25.086883270681053
11	37.12566662108574	21.558274357631408	21.873427780458165	19.44263124082469
12	24.236497939828013	24.200764211574633	29.30875231237213	22.25398553622523
13	30.132950109523232	19.819963930864912	25.523170162207904	24.52391579740395
14	23.677400545423115	19.96792994641231	25.472343126352914	30.88232638181166
15	25.131905188227737	27.259416546909225	23.61315723614447	23.995521028718567
16	25.131389177711444	25.953135924910146	25.16983196117537	23.74564293620304
17	23.303034915851583	26.013122147429364	26.49068988025976	24.19315305645929
18	24.59086816189314	24.736641132746286	28.142052535030665	22.530438170329912
19	24.9558165995423	25.894955739197968	25.940880675148158	23.20834698611158
20	24.836489167649237	24.3059013542696	27.210008539974044	23.647600938107118
21	25.988224640018164	24.946399407619925	24.88447814566461	24.180897806697303
22	24.556424459930493	25.420742074723485	25.88721558145355	24.135617883892476
23	23.515244240677625	24.839585230747	26.108584092943815	25.53658643563156
24	23.743191886250642	26.227782522207804	26.019056268366747	24.009969323174808
25	24.671107797176905	24.800755439395854	25.82671334841804	24.701423415009195
26	23.080247375441513	25.85070783742573	26.965677560508684	24.10336722662408
27	24.711098612189716	25.446542600538198	26.285575700032766	23.556783087239317
28	23.621155399147035	25.205694692057822	26.510556285137092	24.66259362365805
29	23.184481499732964	25.49698262850597	26.957808400135196	24.36072747162587
30	23.980685726375103	25.673458225078626	26.704060228747462	23.64179581979881
31	23.74809398615544	25.480083284097333	25.866188152914553	24.905634576832675
32	23.58064857361793	25.942944717213333	25.941138680406304	24.535268028762427
33	23.329738460069816	25.156028679864495	26.557255236861728	24.95697762320396
34	23.72758256813274	25.975453379739875	26.950713255536147	23.346250796591235
35	24.746058324668656	25.54393958548875	26.454569144119155	23.255432945723435
36	23.4128161531932	26.463341322896163	26.07272136206136	24.051121161849277
37	24.537977083972972	25.9397196514865	25.99454576884277	23.527757495697763
38	23.879934673068636	25.673329222449553	25.91172608097753	24.535010023504277
39	24.184380877682287	25.190343379198065	26.307635149604348	24.317640593515296
40	24.867965809143193	25.65513985175018	25.850578834796657	23.62631550430998
41	22.984140416781695	25.8029768646685	27.277347912350454	23.935534806199353
42	25.110361749172448	26.313053260025438	26.022668341980808	22.553916648821303
43	23.86200330762741	24.96316974939949	26.293831868293477	24.88099507467962
44	23.347411820252898	25.786077520259866	26.47404854110927	24.39246211837797
45	23.63134660684385	25.972486319271187	26.22481546173911	24.171351612145855
46	23.15148669271663	25.28170985465255	26.68874334506829	24.87806010756253
47	23.72890320533246	25.254103256417565	27.062980454786455	23.95401308346352
48	24.081952790197864	26.586538833661432	25.311089840010943	24.020418536129768
49	23.115365909979268	25.89344111086887	26.8720563174604	24.11913666169146
50	23.071762965103453	27.03292280343715	25.128841541996216	24.766472689463182
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	23.0
2	23.0
3	101.0
4	179.0
5	210.5
6	242.0
7	214.5
8	187.0
9	259.5
10	332.0
11	530.5
12	729.0
13	1210.0
14	1691.0
15	2317.5
16	2944.0
17	3437.5
18	3931.0
19	4246.0
20	4561.0
21	5050.5
22	5540.0
23	5904.5
24	6269.0
25	6377.5
26	6486.0
27	8184.0
28	9882.0
29	11504.5
30	13127.0
31	14456.5
32	15786.0
33	18110.5
34	20435.0
35	23254.0
36	26073.0
37	29045.5
38	32018.0
39	33465.0
40	34912.0
41	37163.0
42	39414.0
43	41001.5
44	42589.0
45	48431.0
46	54273.0
47	58478.0
48	62683.0
49	62188.5
50	61694.0
51	56405.0
52	51116.0
53	48991.5
54	46867.0
55	47345.0
56	47823.0
57	46455.5
58	45088.0
59	41330.0
60	37572.0
61	32911.0
62	28250.0
63	24562.0
64	20874.0
65	17963.0
66	15052.0
67	13191.0
68	11330.0
69	10551.5
70	9773.0
71	7537.5
72	5302.0
73	4664.5
74	4027.0
75	2955.5
76	1884.0
77	1698.0
78	1512.0
79	1321.5
80	1131.0
81	875.0
82	619.0
83	530.5
84	442.0
85	366.0
86	290.0
87	220.5
88	151.0
89	88.5
90	26.0
91	19.5
92	13.0
93	10.5
94	8.0
95	5.5
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015480315488829662
2	0.004128084130354577
3	6.450131453679026E-4
4	2.5800525814716104E-4
5	0.0
6	3.8700788722074157E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.2900262907358052E-4
47	1.2900262907358052E-4
48	0.0
49	1.2900262907358052E-4
50	1.2900262907358052E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	775178.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.278162751853095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.64628119055813	49.21487825688025
2	11.856745169299732	14.294056300445943
3	3.0640739647597703	5.540902473945156
4	1.1915864822604583	2.8730657564241606
5	0.6054596733478399	1.8247998364872453
6	0.33820930361888907	1.2231981268638175
7	0.22920099628256904	0.9671070469765299
8	0.1476497985221159	0.7120046868475536
9	0.11346239997713749	0.6155374510833971
>10	0.6395669273416097	7.403837066730978
>50	0.08280988616113169	3.562926011047851
>100	0.07937640634539544	9.219271520261795
>500	0.005148739869404723	2.0412410884911303
>1k	4.290616557837269E-4	0.5071743775142359
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2073	0.2674224500695324	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1849	0.23852586115705038	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	893	0.1151993477627074	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	892	0.11507034513363382	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	885	0.11416732673011877	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	838	0.10810420316366047	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	821	0.1059111584694096	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2900262907358052E-4
2	0.0	0.0	0.0	0.0	1.2900262907358052E-4
3	0.0	0.0	0.0	0.0	1.2900262907358052E-4
4	0.0	0.0	0.0	0.0	1.2900262907358052E-4
5	0.0	0.0	0.0	0.0	1.2900262907358052E-4
6	0.0	0.0	0.0	0.0	2.5800525814716104E-4
7	0.0	0.0	0.0	0.0	2.5800525814716104E-4
8	0.0	0.0	0.0	0.0	2.5800525814716104E-4
9	0.0	0.0	0.0	0.0	2.5800525814716104E-4
10	0.0	0.0	0.0	0.0	2.5800525814716104E-4
11	0.0	0.0	0.0	0.0	2.5800525814716104E-4
12	0.0	0.0	0.0	0.0	3.8700788722074157E-4
13	0.0	0.0	0.0	0.0	5.160105162943221E-4
14	0.0	0.0	0.0	0.0	5.160105162943221E-4
15	0.0	0.0	0.0	0.0	9.030184035150636E-4
16	0.0	0.0	0.0	3.8700788722074157E-4	0.0011610236616622248
17	0.0	0.0	0.0	6.450131453679026E-4	0.0011610236616622248
18	0.0	0.0	0.0	6.450131453679026E-4	0.0011610236616622248
19	0.0	0.0	0.0	7.740157744414831E-4	0.0011610236616622248
20	0.0	0.0	0.0	0.0012900262907358051	0.0012900262907358051
21	0.0	0.0	0.0	0.0014190289198093857	0.0012900262907358051
22	0.0	0.0	0.0	0.0015480315488829663	0.0012900262907358051
23	0.0	0.0	0.0	0.0038700788722074156	0.0012900262907358051
24	0.0	0.0	0.0	0.008127165631635572	0.0012900262907358051
25	0.0	0.0	0.0	0.009417191922371377	0.0012900262907358051
26	0.0	0.0	0.0	0.012513255020137311	0.0014190289198093857
27	0.0	0.0	0.0	0.017028347037712627	0.0014190289198093857
28	0.0	0.0	0.0	0.029154594170629197	0.0014190289198093857
29	0.0	0.0	0.0	0.04605393857926825	0.0014190289198093857
30	0.0	0.0	0.0	0.08359370363968018	0.0014190289198093857
31	0.0	0.0	0.0	0.15699619958254749	0.0014190289198093857
32	0.0	0.0	0.0	0.24252494265833138	0.0014190289198093857
33	0.0	0.0	0.0	0.3191525043280382	0.0014190289198093857
34	0.0	0.0	0.0	0.4095833473086182	0.0014190289198093857
35	0.0	0.0	0.0	0.5167845320687635	0.0014190289198093857
36	0.0	0.0	0.0	0.6657825686487491	0.0014190289198093857
37	0.0	0.0	0.0	0.8840550170412473	0.0014190289198093857
38	0.0	0.0	0.0	1.1556055512411343	0.0015480315488829663
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTATA	60	9.742875E-6	25.668097	2
ATACCTA	75	2.0628358E-6	23.466461	6
GATATAC	435	0.0	22.765762	1
ACTGTTC	465	0.0	22.236362	8
GTCCAAT	60	2.8654028E-4	22.006903	1
GCCGGTT	170	0.0	21.999807	21
ATGCCGG	180	0.0	20.777596	19
AACGAAT	265	0.0	20.754534	31
ATACACT	490	0.0	20.65288	4
CCGGCAT	55	0.0044824127	19.999826	44
CGTCTTA	55	0.0044824127	19.999826	15
GGGCGGA	55	0.0044824127	19.999826	20
CACTGTT	550	0.0	19.999825	7
AACGTAT	145	2.9467628E-10	19.723965	31
CGAATGT	260	0.0	19.461367	33
TGGTATA	205	0.0	19.316902	44
TCCAACG	400	0.0	19.249832	28
CCGTCCA	80	8.98388E-5	19.24983	9
TAGGACG	265	0.0	19.094172	4
TATGCGT	70	8.1202114E-4	18.856977	39
>>END_MODULE