FastQCFastQC Report
Thu 2 Feb 2017
SRR4062401_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062401_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983971
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52640.176409221135192No Hit
GCGCAAGACGGACCAGAGCGAAAGC35290.11826522442744919No Hit
TATCAACGCAGAGTACTTTTTTTTT33830.11337241548259015No Hit
GGGTAGGCACACGCTGAGCCAGTCA31600.10589915250516845No Hit
GTCTTGCGCCGGTCCAAGAATTTCA31280.10482675602410343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA6150.013.59432510
AAGACGG6800.012.5721865
CGCAAGA6450.012.5138442
CAAGACG6850.012.3405064
CGTCGTA3800.012.25074710
AGAATTT7050.012.13084117
GCGTTAT2200.012.0829281
GAATTTC7100.011.91177518
CGACCAT4250.011.84778110
TGTAGGA16050.011.7736082
GGTATCA14700.011.754141
TCGCGTA2300.011.5659229
CCGTCGT3700.011.5547589
AAGAATT7250.011.53386616
CGCCGGT6550.011.4587147
GCGCAAG7150.011.4190311
GTAGGAC15850.011.383933
GTATCAA36050.011.297671
CCGGTCC7750.011.2781069
GACGGAC7550.011.1993587
Produced by FastQC (version 0.11.5)