FastQCFastQC Report
Thu 2 Feb 2017
SRR4062401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983971
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT35270.11819819964738264No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC31250.1047262188540036No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC30650.10271547545200675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39450.021.195831
TAGGACG14350.020.8503574
GTCCTAC17500.020.7472111
GCGAAAT1502.1827873E-1120.53316912
TGTAGGA21700.020.3790842
GTAGGAC20700.020.2996963
CGCAATA6800.019.4116136
CTGTAGG22150.019.1733231
TTAGGAC10800.019.1483153
TCCTACA19250.019.086842
GGTATCA21600.018.0315631
GGCGTTA6500.017.93831842
CTAGCGG7600.017.65775329
TATGGCG19200.017.64569316
AGAGCGA11100.017.44130115
TGGCGAG19300.017.44027518
AAGACGG11750.017.4129185
ACCGTTA1903.274181E-1117.3682828
CGGACCA11150.017.3630899
CCTACAG20300.017.3400543
Produced by FastQC (version 0.11.5)