##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062401_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2983971
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34871552035861	32.0	32.0	32.0	32.0	32.0
2	31.466845689854225	32.0	32.0	32.0	32.0	32.0
3	31.54160848077947	32.0	32.0	32.0	32.0	32.0
4	31.627485320735357	32.0	32.0	32.0	32.0	32.0
5	31.569223025290796	32.0	32.0	32.0	32.0	32.0
6	35.19130916486789	36.0	36.0	36.0	36.0	36.0
7	35.203566656646466	36.0	36.0	36.0	36.0	36.0
8	35.139858262697594	36.0	36.0	36.0	36.0	36.0
9	35.27875941153584	36.0	36.0	36.0	36.0	36.0
10	35.155184819155416	36.0	36.0	36.0	36.0	36.0
11	35.273030468459645	36.0	36.0	36.0	36.0	36.0
12	35.193483113609346	36.0	36.0	36.0	36.0	36.0
13	35.23510114541998	36.0	36.0	36.0	36.0	36.0
14	35.200187267235506	36.0	36.0	36.0	36.0	36.0
15	35.16113293326242	36.0	36.0	36.0	36.0	36.0
16	35.17851513972489	36.0	36.0	36.0	36.0	36.0
17	35.15361710954966	36.0	36.0	36.0	36.0	36.0
18	35.16419328472026	36.0	36.0	36.0	36.0	36.0
19	35.153737754153774	36.0	36.0	36.0	36.0	36.0
20	35.147332866170615	36.0	36.0	36.0	36.0	36.0
21	35.14416695068417	36.0	36.0	36.0	36.0	36.0
22	35.13392556429	36.0	36.0	36.0	36.0	36.0
23	35.09385915613791	36.0	36.0	36.0	36.0	36.0
24	35.07654766081842	36.0	36.0	36.0	36.0	36.0
25	35.0521556677327	36.0	36.0	36.0	36.0	36.0
26	35.01084226354747	36.0	36.0	36.0	36.0	36.0
27	34.99314470549479	36.0	36.0	36.0	36.0	36.0
28	34.988496201873275	36.0	36.0	36.0	36.0	36.0
29	34.96784050515236	36.0	36.0	36.0	36.0	36.0
30	34.950280012774925	36.0	36.0	36.0	36.0	36.0
31	34.952323933443054	36.0	36.0	36.0	36.0	36.0
32	34.917961669198526	36.0	36.0	36.0	32.0	36.0
33	34.911656313013765	36.0	36.0	36.0	32.0	36.0
34	34.90369309889406	36.0	36.0	36.0	32.0	36.0
35	34.881314865325436	36.0	36.0	36.0	32.0	36.0
36	34.859207076744376	36.0	36.0	36.0	32.0	36.0
37	34.85220332235132	36.0	36.0	36.0	32.0	36.0
38	34.821019708301456	36.0	36.0	36.0	32.0	36.0
39	34.807077883799806	36.0	36.0	36.0	32.0	36.0
40	34.812499853383294	36.0	36.0	36.0	32.0	36.0
41	34.78671273950049	36.0	36.0	36.0	32.0	36.0
42	34.77467207288543	36.0	36.0	36.0	32.0	36.0
43	34.759942707218	36.0	36.0	36.0	32.0	36.0
44	34.726909879486094	36.0	36.0	36.0	32.0	36.0
45	34.70950957633301	36.0	36.0	36.0	32.0	36.0
46	34.69025201652429	36.0	36.0	36.0	32.0	36.0
47	34.67222369118198	36.0	36.0	36.0	32.0	36.0
48	34.631987375212425	36.0	36.0	36.0	32.0	36.0
49	34.61819937258103	36.0	36.0	36.0	32.0	36.0
50	34.126134268731164	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	15.0
21	48.0
22	176.0
23	494.0
24	1397.0
25	3473.0
26	7075.0
27	13761.0
28	23610.0
29	38149.0
30	57603.0
31	84903.0
32	128529.0
33	218315.0
34	499383.0
35	1907034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.271853706753205	17.807652589067757	11.599112045737085	26.32138165844195
2	16.424769282277442	19.156851856382428	37.321861319018	27.09651754232213
3	19.56356653574445	21.99835118433223	28.290459657636163	30.147622622287162
4	12.619115840835935	14.705836026897098	35.20580837911973	37.469239753147235
5	14.61780292100694	36.28409927576374	33.39791170892747	15.700186094301854
6	36.21159003860308	34.592331865149625	15.552425485738913	13.643652610508388
7	31.500909358703556	29.88035071386418	19.341273759027818	19.27746616840445
8	28.367266303861534	34.17050634875473	18.261370502595366	19.200856844788373
9	26.80863855580366	14.164179209516448	18.403630598286647	40.62355163639325
10	16.07978093620883	26.827707105732596	31.293266590057346	25.799245368001227
11	38.46227057836688	20.746381248343233	21.49685771074853	19.294490462541358
12	24.60131147387156	23.24225000846188	28.631511499273955	23.524927018392606
13	29.85199923189602	19.085172074393483	25.21143804681748	25.85139064689302
14	22.810912036343517	19.715171494629136	24.55992367218046	32.913992796846884
15	25.07356807422056	28.097223464973354	21.5360001823074	25.293208278498685
16	25.67253502128539	26.090166425880145	23.928248632443143	24.309049920391317
17	23.952042429366774	26.552335796829123	24.9619383030197	24.5336834707844
18	24.673363112443116	25.190392265876575	26.519996340447012	23.616248281233297
19	25.6240090805172	24.839785641348392	25.02319895200054	24.513006326133866
20	25.620959453024174	24.8137130019025	25.01639593682378	24.548931608249543
21	26.264698953173472	24.264277367306857	24.536967685007664	24.93405599451201
22	25.617876313141114	24.83569712976433	25.095150053401994	24.45127650369256
23	24.30888235844115	24.687639390597294	25.748340047540673	25.25513820342088
24	24.869745718038143	24.929062648397053	25.50658166584059	24.69460996772422
25	24.801346929980216	24.874906626103268	25.446929611581346	24.876816832335166
26	24.173190691196396	25.80581379644775	25.853770026585376	24.167225485770473
27	24.930537193558518	25.06170468814878	25.35332950621839	24.654428612074312
28	24.292796411225176	25.26720266383286	25.501722369285762	24.938278555656204
29	24.314445415186675	24.956274709104076	25.382183674037044	25.347096201672205
30	24.023323282967564	25.441902753076352	25.88962828392099	24.645145680035093
31	25.126216038962845	24.902922984171095	24.977823175895473	24.993037800970587
32	24.635259524975275	25.160968387427356	24.92872752449672	25.275044563100646
33	24.21585196370876	24.9630442118908	25.63051048418366	25.190593340216775
34	24.97091962354862	25.031308950388592	25.68064501967345	24.317126406389338
35	24.999941353317443	25.010799367688225	25.798809706930797	24.190449572063535
36	23.957638998502333	25.485938033580087	25.595322474648714	24.96110049326887
37	25.025477794522804	24.90922331349735	25.08858162495547	24.97671726702438
38	24.401242505372874	25.03271647076999	25.50376662507779	25.062274398779344
39	25.357853678872882	24.491223272612235	25.12645062569308	25.024472422821802
40	25.36375185951874	24.71290773268239	25.620791891074006	24.302548516724862
41	24.500137568361087	24.863378363931822	26.32207216491045	24.314411902796643
42	25.2820486526176	25.346928639722034	25.530777611444616	23.84024509621575
43	24.41441287465595	24.59896560656923	25.88614299535753	25.100478523417287
44	23.83669278287222	25.15751661125393	25.613653751996917	25.392136853876927
45	24.002646138317026	25.26167311947737	25.65648258645945	25.079198155746152
46	24.129974714850878	24.648445943568372	25.769739256325057	25.45184008525569
47	24.446015058457338	24.638074565738073	26.018986109449454	24.896924266355136
48	24.664348279524166	25.178193755904466	25.193542430539708	24.963915534031663
49	24.204524775877516	25.135968144462527	25.421996393396583	25.23751068626337
50	23.805258161021	25.62112701497434	25.423973624408546	25.149641199596108
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	23.0
2	36.0
3	90.0
4	144.0
5	181.5
6	219.0
7	225.5
8	232.0
9	346.0
10	460.0
11	710.5
12	961.0
13	1674.5
14	2388.0
15	3372.0
16	4356.0
17	5083.0
18	5810.0
19	6393.5
20	6977.0
21	8405.5
22	9834.0
23	11313.5
24	12793.0
25	15380.0
26	17967.0
27	23270.5
28	28574.0
29	34537.0
30	40500.0
31	46764.5
32	53029.0
33	62197.5
34	71366.0
35	83678.5
36	95991.0
37	107515.5
38	119040.0
39	126167.0
40	133294.0
41	140867.0
42	148440.0
43	154647.5
44	160855.0
45	174365.0
46	187875.0
47	199803.5
48	211732.0
49	220429.5
50	229127.0
51	225937.5
52	222748.0
53	219557.0
54	216366.0
55	215156.5
56	213947.0
57	203508.5
58	193070.0
59	179424.0
60	165778.0
61	145962.5
62	126147.0
63	109690.5
64	93234.0
65	79590.0
66	65946.0
67	56829.0
68	47712.0
69	42651.5
70	37591.0
71	29087.0
72	20583.0
73	18264.5
74	15946.0
75	11747.5
76	7549.0
77	6709.0
78	5869.0
79	4888.0
80	3907.0
81	3149.0
82	2391.0
83	1973.5
84	1556.0
85	1277.5
86	999.0
87	722.5
88	446.0
89	269.5
90	93.0
91	70.0
92	47.0
93	31.0
94	15.0
95	11.0
96	7.0
97	9.5
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01621999677610808
2	0.0043566107043265506
3	3.686362903660927E-4
4	2.0107434019968692E-4
5	0.0
6	4.0214868039937385E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.0107434019968692E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2983971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.369372745760835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.08959794476996	40.60854114789505
2	13.997539724036887	14.940798301114377
3	4.389808737730597	7.028440164196262
4	1.8608398792382388	3.9724742854096857
5	0.9407349638193143	2.510321746952141
6	0.5752208232206778	1.8419504715352653
7	0.39919690530727614	1.4913421906808748
8	0.2888545648468351	1.2332789552500227
9	0.20392829029004925	0.9795172444105824
>10	1.1131274246043426	10.618938075387508
>50	0.07430854911205102	2.764909797235969
>100	0.05523528773274549	6.083238929091108
>500	0.008263611919201146	3.0452524006468082
>1k	0.0033432933717534715	2.880996290194474
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3527	0.11819819964738264	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3125	0.1047262188540036	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3065	0.10271547545200675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.70247800665623E-5
2	0.0	0.0	0.0	0.0	6.70247800665623E-5
3	0.0	0.0	0.0	0.0	6.70247800665623E-5
4	0.0	0.0	0.0	0.0	6.70247800665623E-5
5	0.0	0.0	0.0	0.0	6.70247800665623E-5
6	0.0	0.0	0.0	0.0	6.70247800665623E-5
7	0.0	0.0	0.0	0.0	6.70247800665623E-5
8	0.0	0.0	0.0	0.0	6.70247800665623E-5
9	0.0	0.0	0.0	0.0	6.70247800665623E-5
10	0.0	0.0	0.0	0.0	6.70247800665623E-5
11	0.0	0.0	0.0	0.0	6.70247800665623E-5
12	0.0	0.0	0.0	3.351239003328115E-5	2.345867302329681E-4
13	0.0	0.0	0.0	3.351239003328115E-5	2.680991202662492E-4
14	0.0	0.0	0.0	6.70247800665623E-5	2.680991202662492E-4
15	0.0	0.0	0.0	6.70247800665623E-5	3.686362903660927E-4
16	0.0	0.0	0.0	1.6756195016640578E-4	3.686362903660927E-4
17	0.0	0.0	0.0	2.0107434019968692E-4	4.35661070432655E-4
18	0.0	0.0	0.0	3.016115102995304E-4	4.35661070432655E-4
19	0.0	0.0	0.0	3.3512390033281156E-4	4.35661070432655E-4
20	0.0	0.0	0.0	4.0214868039937385E-4	4.691734604659362E-4
21	0.0	0.0	0.0	4.691734604659362E-4	4.691734604659362E-4
22	0.0	0.0	0.0	8.7132214086531E-4	4.691734604659362E-4
23	0.0	0.0	0.0	0.0013404956013312462	4.691734604659362E-4
24	0.0	0.0	0.0	0.0018096690617971822	4.691734604659362E-4
25	0.0	0.0	0.0	0.0021447929621299937	4.691734604659362E-4
26	0.0	0.0	0.0	0.0029155779328954605	4.691734604659362E-4
27	0.0	0.0	0.0	0.004289585924259987	4.691734604659362E-4
28	0.0	0.0	0.0	0.0110255763209495	4.691734604659362E-4
29	0.0	0.0	0.0	0.0261396642259593	4.691734604659362E-4
30	0.0	0.0	0.0	0.053619824053249845	4.691734604659362E-4
31	0.0	0.0	0.0	0.10596617728523501	4.691734604659362E-4
32	0.0	0.0	0.0	0.16836624752720453	5.026858504992173E-4
33	0.0	0.0	0.0	0.23180520186020576	5.361982405324984E-4
34	0.0	0.0	0.0	0.3042254767221263	6.36735410632342E-4
35	0.0	0.0	0.0	0.3868000057641311	6.36735410632342E-4
36	0.0	0.0	0.0	0.5054673788719797	6.36735410632342E-4
37	0.0	0.0	0.0	0.6783577990536771	6.36735410632342E-4
38	0.0	0.0	0.0	0.9057393654294897	6.36735410632342E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3945	0.0	21.19583	1
TAGGACG	1435	0.0	20.850357	4
GTCCTAC	1750	0.0	20.747211	1
GCGAAAT	150	2.1827873E-11	20.533169	12
TGTAGGA	2170	0.0	20.379084	2
GTAGGAC	2070	0.0	20.299696	3
CGCAATA	680	0.0	19.41161	36
CTGTAGG	2215	0.0	19.173323	1
TTAGGAC	1080	0.0	19.148315	3
TCCTACA	1925	0.0	19.08684	2
GGTATCA	2160	0.0	18.031563	1
GGCGTTA	650	0.0	17.938318	42
CTAGCGG	760	0.0	17.657753	29
TATGGCG	1920	0.0	17.645693	16
AGAGCGA	1110	0.0	17.441301	15
TGGCGAG	1930	0.0	17.440275	18
AAGACGG	1175	0.0	17.412918	5
ACCGTTA	190	3.274181E-11	17.368282	8
CGGACCA	1115	0.0	17.363089	9
CCTACAG	2030	0.0	17.340054	3
>>END_MODULE