FastQCFastQC Report
Thu 2 Feb 2017
SRR4062400_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062400_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312239
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23660.1803025211108647No Hit
TATCAACGCAGAGTACTTTTTTTTT15940.12147177457764935No Hit
GCGCAAGACGGACCAGAGCGAAAGC15910.12124315768697624No Hit
GATTAAGAGGGACGGCCGGGGGCAT14310.10905025685107668No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13760.1048589471887362No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13470.1026489839122294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG350.002176015616.2789172
CGTTATT1151.8189894E-1214.86335852
GCGTTAT1101.2732926E-1114.675691
CGAACGA1006.566552E-1014.25329116
ATACGCG551.9635586E-413.8150474
TAACGAA1051.36788E-913.57300913
GCACCGT655.4491655E-513.1543746
ATAACGA1102.743036E-912.95506612
CGCGTAT604.0898804E-412.6671757
ACGCGTA604.0898804E-412.6671756
ATTGGAC604.1030603E-412.6623463
GTTATAC604.1057006E-412.661381
AACGAAC1155.311449E-912.39274814
ACTCTAA1800.012.13891310
GAGCGTT550.003067083212.0913947
ATATACG2150.011.9307126
ACGAACG1209.982614E-911.87683715
GCATCGC2000.011.87638414
CGCATCG1850.011.81218613
CGGTTCT3150.011.76237712
Produced by FastQC (version 0.11.5)