##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062400_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1312239
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20569880943944	32.0	32.0	32.0	32.0	32.0
2	30.96059787889249	32.0	32.0	32.0	32.0	32.0
3	30.96327422062597	32.0	32.0	32.0	32.0	32.0
4	30.91759351764427	32.0	32.0	32.0	32.0	32.0
5	30.879406114282535	32.0	32.0	32.0	32.0	32.0
6	34.3682027435551	36.0	36.0	36.0	32.0	36.0
7	34.337100939691624	36.0	36.0	36.0	32.0	36.0
8	34.329869787439634	36.0	36.0	36.0	32.0	36.0
9	34.431517429370714	36.0	36.0	36.0	32.0	36.0
10	34.18804196491645	36.0	36.0	36.0	32.0	36.0
11	34.429889677109124	36.0	36.0	36.0	32.0	36.0
12	34.25537116333229	36.0	36.0	36.0	32.0	36.0
13	34.359828506849745	36.0	36.0	36.0	32.0	36.0
14	34.26900739880464	36.0	36.0	36.0	32.0	36.0
15	34.214311569767396	36.0	36.0	36.0	32.0	36.0
16	34.2200536640048	36.0	36.0	36.0	32.0	36.0
17	34.15260253658061	36.0	36.0	36.0	32.0	36.0
18	34.16557654512631	36.0	36.0	36.0	32.0	36.0
19	34.148129266086436	36.0	36.0	36.0	32.0	36.0
20	34.11949728669853	36.0	36.0	36.0	32.0	36.0
21	34.10286312173316	36.0	36.0	36.0	32.0	36.0
22	34.05664364494577	36.0	36.0	36.0	32.0	36.0
23	34.038124152688646	36.0	36.0	36.0	32.0	36.0
24	34.00893739631272	36.0	36.0	36.0	32.0	36.0
25	33.43143817551528	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	55.0
5	123.0
6	200.0
7	45.0
8	200.0
9	220.0
10	146.0
11	55.0
12	96.0
13	73.0
14	211.0
15	408.0
16	633.0
17	895.0
18	1072.0
19	1415.0
20	1720.0
21	2364.0
22	3004.0
23	4330.0
24	5991.0
25	8482.0
26	11844.0
27	16285.0
28	22572.0
29	31634.0
30	43118.0
31	62261.0
32	97076.0
33	149656.0
34	343801.0
35	502242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.93884761594108	17.619797398432475	11.14506772184506	26.296287263781387
2	17.285690225811443	20.030467893257207	36.41934429252836	26.264497588402996
3	19.19013172133971	23.501254416368685	28.38037013665975	28.92824372563186
4	13.08838671962233	15.279019481860704	35.43250553907995	36.20008825943701
5	15.161679349044132	35.84000231743703	33.23319057717904	15.765127756339792
6	34.26875876270425	35.0059123237834	16.75364250225095	13.971686411261395
7	30.134224400748522	30.223748004020173	20.85450050557197	18.787527089659335
8	28.071984532249267	32.27062462102867	19.74395838656411	19.913432460157956
9	27.4790201842532	14.135918390385516	18.73584119869842	39.64922022666286
10	16.296812261156095	26.230353670175877	31.21623570179006	26.25659836687797
11	37.39823255962994	21.054864199452357	22.17608942946146	19.37081381145624
12	24.67507792559584	23.64367495965419	28.427257362395036	23.253989752354936
13	29.285008564028033	19.354245583517393	25.233021671377838	26.12772418107674
14	23.52970349153934	19.679869744417672	24.891008610968264	31.899418153074727
15	25.344427855724415	26.95428811631397	22.05574165946367	25.645542368497942
16	25.93264224987641	25.45728078901916	23.638380460057736	24.971696501046694
17	24.23816078202777	25.576357882079343	25.046236811826855	25.13924452406603
18	25.083761072446936	24.507753478821858	26.120712085477116	24.28777336325409
19	25.709059943320987	24.776008021133304	25.22498393789421	24.289948097651497
20	25.730009430305767	24.202391452297604	24.768514986251287	25.29908413114534
21	26.901756535791865	24.01664592538786	24.18824789732266	24.893349641497615
22	25.97641061255328	24.237160726073867	24.708414260475376	25.07801440089748
23	24.64588283953048	23.95161327246837	25.294442638438024	26.108061249563125
24	24.774190791516883	24.816159217525406	25.03363197047865	25.376018020479062
25	24.859336443420972	24.41685856731478	25.19079426266659	25.53301072659766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	104.0
2	351.5
3	599.0
4	599.0
5	599.0
6	1368.5
7	2138.0
8	2138.0
9	2138.0
10	2423.0
11	2708.0
12	2708.0
13	2708.0
14	3306.0
15	3904.0
16	3904.0
17	3904.0
18	6329.0
19	8754.0
20	8754.0
21	8754.0
22	14027.0
23	19300.0
24	19300.0
25	19300.0
26	28694.5
27	38089.0
28	38089.0
29	38089.0
30	47162.0
31	56235.0
32	56235.0
33	56235.0
34	69445.0
35	82655.0
36	82655.0
37	82655.0
38	93201.0
39	103747.0
40	103747.0
41	103747.0
42	118116.5
43	132486.0
44	132486.0
45	132486.0
46	149017.0
47	165548.0
48	165548.0
49	165548.0
50	168578.5
51	171609.0
52	171609.0
53	171609.0
54	160148.0
55	148687.0
56	148687.0
57	148687.0
58	137749.0
59	126811.0
60	126811.0
61	126811.0
62	113431.5
63	100052.0
64	100052.0
65	100052.0
66	83734.5
67	67417.0
68	67417.0
69	67417.0
70	52004.0
71	36591.0
72	36591.0
73	36591.0
74	29128.0
75	21665.0
76	21665.0
77	21665.0
78	17617.0
79	13569.0
80	13569.0
81	13569.0
82	9579.0
83	5589.0
84	5589.0
85	5589.0
86	4090.0
87	2591.0
88	2591.0
89	2591.0
90	1693.0
91	795.0
92	795.0
93	795.0
94	455.0
95	115.0
96	115.0
97	115.0
98	298.0
99	481.0
100	481.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008154002434007828
2	0.0028958139485261453
3	0.005715422266827918
4	0.015088714784425705
5	0.03391150544984564
6	0.04358962048834092
7	0.06500340258138951
8	0.0856551283721944
9	0.092742251983061
10	0.1141560340761096
11	0.11659461424328953
12	0.12909233760008657
13	0.1161373804619433
14	0.12078592390562999
15	0.10988851878354478
16	0.11026954693466662
17	0.12154798020787372
18	0.12695857995380413
19	0.12962577701165717
20	0.11971904508248879
21	0.12558687860976545
22	0.14509551994720474
23	0.13694151751319691
24	0.13183574028816397
25	0.12688237432357977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1312239.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.81354844165855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.50679410544282	46.875037436755235
2	10.988378557335226	12.48577554924894
3	2.7645836549422973	4.711974222032449
4	1.1694849561383156	2.6577036082942045
5	0.6170353615934299	1.7527984203052314
6	0.3820370295635724	1.3022927571370417
7	0.2608046246345854	1.0372065322839916
8	0.18227547833792548	0.828457337462257
9	0.13714430325619953	0.7012489064889241
>10	0.8030711434908009	8.61297118666772
>50	0.0884059318037904	3.5063615866660585
>100	0.08978506897366056	10.724691419539605
>500	0.007649838365472118	2.9469910486780804
>1k	0.002549946121824039	1.8564899884402044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2366	0.1803025211108647	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1594	0.12147177457764935	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1591	0.12124315768697624	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1431	0.10905025685107668	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1376	0.1048589471887362	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1347	0.1026489839122294	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.620563022437223E-5
2	0.0	0.0	0.0	0.0	7.620563022437223E-5
3	0.0	0.0	0.0	0.0	7.620563022437223E-5
4	0.0	0.0	0.0	0.0	7.620563022437223E-5
5	0.0	0.0	0.0	0.0	7.620563022437223E-5
6	0.0	0.0	0.0	0.0	7.620563022437223E-5
7	0.0	0.0	0.0	0.0	7.620563022437223E-5
8	0.0	0.0	0.0	0.0	7.620563022437223E-5
9	0.0	0.0	0.0	0.0	1.5241126044874447E-4
10	0.0	0.0	0.0	0.0	1.5241126044874447E-4
11	0.0	0.0	0.0	0.0	1.5241126044874447E-4
12	0.0	0.0	0.0	0.0	2.286168906731167E-4
13	0.0	0.0	0.0	0.0	2.286168906731167E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	35	0.0021760156	16.278917	2
CGTTATT	115	1.8189894E-12	14.8633585	2
GCGTTAT	110	1.2732926E-11	14.67569	1
CGAACGA	100	6.566552E-10	14.253291	16
ATACGCG	55	1.9635586E-4	13.815047	4
TAACGAA	105	1.36788E-9	13.573009	13
GCACCGT	65	5.4491655E-5	13.154374	6
ATAACGA	110	2.743036E-9	12.955066	12
CGCGTAT	60	4.0898804E-4	12.667175	7
ACGCGTA	60	4.0898804E-4	12.667175	6
ATTGGAC	60	4.1030603E-4	12.662346	3
GTTATAC	60	4.1057006E-4	12.66138	1
AACGAAC	115	5.311449E-9	12.392748	14
ACTCTAA	180	0.0	12.138913	10
GAGCGTT	55	0.0030670832	12.091394	7
ATATACG	215	0.0	11.930712	6
ACGAACG	120	9.982614E-9	11.876837	15
GCATCGC	200	0.0	11.876384	14
CGCATCG	185	0.0	11.812186	13
CGGTTCT	315	0.0	11.762377	12
>>END_MODULE