FastQCFastQC Report
Thu 2 Feb 2017
SRR4062400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312239
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13910.10600203164210178No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13580.10348724584469748No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13480.10272518954245377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG2250.022.4887314
TAGATCG550.004483992719.9998635
GTATCAA20300.019.7285121
GTATTAC1051.789127E-618.8613241
TGGTCGA851.4310266E-418.11752110
TATACTG2200.017.9998765
GTATTAG3700.017.8417931
GATATAC2850.017.7583221
ATACACT3650.017.479334
TAAACGC2900.017.44815428
CGTCGTA3050.017.31135610
TGTAGGA3850.016.5725772
GCGACAC800.001988564916.49988626
AGGACGT2850.016.2104135
GTCCTAA3000.016.1369111
CGACGGT5200.016.0768137
GTAGGAC4800.016.0415553
TAGTACG1105.618502E-516.0011082
GACGGTA5400.015.8887798
TACGACG5150.015.8057155
Produced by FastQC (version 0.11.5)