FastQCFastQC Report
Thu 2 Feb 2017
SRR4062399_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062399_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199621
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42860.35727950744443454No Hit
GTACATGGAAGCAGTGGTATCAACG40880.3407742945480281No Hit
TCCATGTACTCTGCGTTGATACCAC34650.28884122568711285No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33240.2770875134730052No Hit
TATCAACGCAGAGTACTTTTTTTTT30440.25374680836697594No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23620.19689551950157594No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18210.15179794285028356No Hit
GAGTACATGGAAGCAGTGGTATCAA17210.14346197674098735No Hit
GGTATCAACGCAGAGTACTTTTTTT14970.1247894126561639No Hit
CATGTACTCTGCGTTGATACCACTG14890.1241225353674202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA402.7585597E-416.62649710
TCGCGTA402.759438E-416.6258039
GCGTAAC456.758254E-414.77849211
CGAACGA801.2848432E-714.25247316
TCGCCAG1301.8189894E-1213.88760517
ATCGCCA1450.013.76100816
TCTATAC1205.2750693E-1113.4528073
GATTGCG852.6943053E-713.41297310
CGACCAT1102.739398E-912.95571210
CGCCAGT1457.2759576E-1212.45147618
AACCGCG853.93538E-612.2957387
GCATCGC1551.8189894E-1212.25916914
TAGTACT701.0917061E-412.2128374
GGTCGCG550.003064713212.0925037
CGGAATT550.003065671512.09199815
CGACTTT1103.805326E-812.09199813
ACGAACG951.0379717E-612.001080515
CGTCGTA1209.988071E-911.8760710
CTATACC907.466313E-611.6097344
CGTCTTA1406.7848305E-1011.53675415