##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062399_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1199621
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.080114469486613	32.0	32.0	32.0	32.0	32.0
2	30.773619334773233	32.0	32.0	32.0	32.0	32.0
3	30.78547307858065	32.0	32.0	32.0	32.0	32.0
4	30.822821541136744	32.0	32.0	32.0	32.0	32.0
5	30.661375551111558	32.0	32.0	32.0	32.0	32.0
6	34.27238269420092	36.0	36.0	36.0	32.0	36.0
7	34.126008964497956	36.0	36.0	36.0	32.0	36.0
8	34.121188275296944	36.0	36.0	36.0	32.0	36.0
9	34.25338419384122	36.0	36.0	36.0	32.0	36.0
10	33.969397834816164	36.0	36.0	36.0	32.0	36.0
11	34.29228981486653	36.0	36.0	36.0	32.0	36.0
12	34.07397753123695	36.0	36.0	36.0	32.0	36.0
13	34.18904387302323	36.0	36.0	36.0	32.0	36.0
14	34.10924783744199	36.0	36.0	36.0	32.0	36.0
15	34.06633094952489	36.0	36.0	36.0	32.0	36.0
16	34.054261304195244	36.0	36.0	36.0	32.0	36.0
17	33.98756107137171	36.0	36.0	36.0	32.0	36.0
18	34.00268251389397	36.0	36.0	36.0	32.0	36.0
19	33.96983964101995	36.0	36.0	36.0	32.0	36.0
20	33.95057605693798	36.0	36.0	36.0	32.0	36.0
21	33.91260739850336	36.0	36.0	36.0	32.0	36.0
22	33.87454120926526	36.0	36.0	36.0	32.0	36.0
23	33.850129332514186	36.0	36.0	36.0	32.0	36.0
24	33.830697361916805	36.0	36.0	36.0	32.0	36.0
25	33.35380257598025	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	52.0
5	116.0
6	198.0
7	66.0
8	158.0
9	205.0
10	165.0
11	50.0
12	101.0
13	64.0
14	324.0
15	406.0
16	598.0
17	758.0
18	1051.0
19	1486.0
20	2130.0
21	2913.0
22	4147.0
23	5895.0
24	7833.0
25	10686.0
26	14594.0
27	18748.0
28	25250.0
29	33424.0
30	43180.0
31	58846.0
32	84472.0
33	121146.0
34	268047.0
35	492504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.382713990954755	17.784122881974117	11.417519434776265	25.415643692294864
2	16.12278004895063	20.691727743802808	37.951742949175376	25.233749258071185
3	18.90733569811887	25.19663705821791	28.505141532831196	27.390885710832023
4	12.19186430899318	15.814331962405506	36.552248524508705	35.44155520409261
5	13.847862724708069	37.32724466233644	34.01711354632007	14.807779066635424
6	32.872669227548776	35.98500833148467	17.650070971906466	13.492251469060085
7	29.021755722962165	31.16629727288121	21.632684384777917	18.1792626193787
8	27.611740431634992	33.17250131199215	20.336587518845548	18.879170737527314
9	27.450483008143173	14.486322487727662	19.200668876785986	38.862525627343175
10	15.525708286108694	27.326415028201247	32.56371779057132	24.584158895118737
11	36.34324154018854	21.699265126255565	22.93866036814629	19.018832965409604
12	24.27067282570771	24.334611551373847	29.817565954548414	21.57714966837003
13	29.403659234802294	20.423744798334802	25.760949578274943	24.411646388587968
14	22.937511893673065	20.5218860277302	26.186279124892746	30.35432295370398
15	24.596258985120564	28.20875106719823	23.596034487507	23.598955460174206
16	24.68654758367911	26.431733134824597	25.23266915247674	23.649050129019553
17	22.82280101692482	26.519336094320945	26.783499510066587	23.874363378687647
18	23.71322041085131	25.25839928083075	28.27789180576087	22.750488502557072
19	24.570181259677547	26.05840543236464	26.439038234398023	22.932375073559793
20	24.686996358549727	24.979030154012563	26.966679436363926	23.36729405107378
21	25.52773608938585	24.94595348076278	25.359962338549064	24.166348091302307
22	24.54095390754794	25.763434935423813	25.93374575266528	23.76186540436296
23	23.184397648594818	25.531211547997284	26.52434799594631	24.760042807461588
24	23.586507399894824	26.313324819071948	26.46858487967345	23.631582901359778
25	24.05449261039336	25.55227981843587	26.396329319535173	23.996898251635596
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	446.0
1	446.0
2	832.5
3	1219.0
4	1219.0
5	1219.0
6	3140.0
7	5061.0
8	5061.0
9	5061.0
10	5059.0
11	5057.0
12	5057.0
13	5057.0
14	5072.0
15	5087.0
16	5087.0
17	5087.0
18	8255.5
19	11424.0
20	11424.0
21	11424.0
22	17569.0
23	23714.0
24	23714.0
25	23714.0
26	34222.0
27	44730.0
28	44730.0
29	44730.0
30	54595.0
31	64460.0
32	64460.0
33	64460.0
34	75227.0
35	85994.0
36	85994.0
37	85994.0
38	94583.5
39	103173.0
40	103173.0
41	103173.0
42	115078.5
43	126984.0
44	126984.0
45	126984.0
46	146890.0
47	166796.0
48	166796.0
49	166796.0
50	160549.0
51	154302.0
52	154302.0
53	154302.0
54	138511.0
55	122720.0
56	122720.0
57	122720.0
58	111039.0
59	99358.0
60	99358.0
61	99358.0
62	87279.5
63	75201.0
64	75201.0
65	75201.0
66	62236.5
67	49272.0
68	49272.0
69	49272.0
70	37484.5
71	25697.0
72	25697.0
73	25697.0
74	19853.0
75	14009.0
76	14009.0
77	14009.0
78	11242.5
79	8476.0
80	8476.0
81	8476.0
82	6031.5
83	3587.0
84	3587.0
85	3587.0
86	2597.5
87	1608.0
88	1608.0
89	1608.0
90	1081.0
91	554.0
92	554.0
93	554.0
94	345.5
95	137.0
96	137.0
97	137.0
98	346.0
99	555.0
100	555.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00800252746492434
2	0.0037511847491832834
3	0.006335334243065102
4	0.015838335607662752
5	0.037845286136204685
6	0.04626461190659383
7	0.0675213254852991
8	0.0885279600807255
9	0.09953143534499646
10	0.12062142960151581
11	0.12137166655135245
12	0.13329209808764603
13	0.12062142960151581
14	0.1254562899449076
15	0.11586992891921698
16	0.1127856214587774
17	0.12495613197834983
18	0.13104138723813605
19	0.13470921232622637
20	0.12270542112883986
21	0.13162490486578676
22	0.15096434623935392
23	0.1416280641969422
24	0.1359596072426208
25	0.13229178215453047
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1199621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.0561013108172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47112764479314	43.753704548966176
2	12.856921810789215	14.157039795201326
3	3.589668997984548	5.928995400760108
4	1.4047373059237944	3.093574377200994
5	0.7067707470445922	1.9456020926404514
6	0.4225923805925155	1.3959773351448574
7	0.26002066333996326	1.0021006788625635
8	0.1841759904259052	0.8112009590326988
9	0.139645057166763	0.6919481173435348
>10	0.768761378328398	8.042308696884401
>50	0.09143836429475595	3.53054854517178
>100	0.0942722029016231	10.176501352710583
>500	0.00819757917537129	3.1183335235055654
>1k	0.001669877239427485	2.352164576575104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4286	0.35727950744443454	No Hit
GTACATGGAAGCAGTGGTATCAACG	4088	0.3407742945480281	No Hit
TCCATGTACTCTGCGTTGATACCAC	3465	0.28884122568711285	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3324	0.2770875134730052	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3044	0.25374680836697594	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2362	0.19689551950157594	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1821	0.15179794285028356	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1721	0.14346197674098735	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1497	0.1247894126561639	No Hit
CATGTACTCTGCGTTGATACCACTG	1489	0.1241225353674202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.335966109296187E-5	0.0
5	0.0	0.0	0.0	8.335966109296187E-5	0.0
6	0.0	0.0	0.0	8.335966109296187E-5	8.335966109296187E-5
7	0.0	0.0	0.0	8.335966109296187E-5	8.335966109296187E-5
8	0.0	0.0	0.0	1.6671932218592373E-4	8.335966109296187E-5
9	0.0	0.0	0.0	2.500789832788856E-4	8.335966109296187E-5
10	8.335966109296187E-5	0.0	0.0	5.83517627650733E-4	8.335966109296187E-5
11	8.335966109296187E-5	0.0	0.0	5.83517627650733E-4	8.335966109296187E-5
12	8.335966109296187E-5	0.0	0.0	6.668772887436949E-4	1.6671932218592373E-4
13	8.335966109296187E-5	0.0	0.0	6.668772887436949E-4	1.6671932218592373E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	40	2.7585597E-4	16.626497	10
TCGCGTA	40	2.759438E-4	16.625803	9
GCGTAAC	45	6.758254E-4	14.778492	11
CGAACGA	80	1.2848432E-7	14.252473	16
TCGCCAG	130	1.8189894E-12	13.887605	17
ATCGCCA	145	0.0	13.761008	16
TCTATAC	120	5.2750693E-11	13.452807	3
GATTGCG	85	2.6943053E-7	13.412973	10
CGACCAT	110	2.739398E-9	12.955712	10
CGCCAGT	145	7.2759576E-12	12.451476	18
AACCGCG	85	3.93538E-6	12.295738	7
GCATCGC	155	1.8189894E-12	12.259169	14
TAGTACT	70	1.0917061E-4	12.212837	4
GGTCGCG	55	0.0030647132	12.092503	7
CGGAATT	55	0.0030656715	12.091998	15
CGACTTT	110	3.805326E-8	12.091998	13
ACGAACG	95	1.0379717E-6	12.0010805	15
CGTCGTA	120	9.988071E-9	11.87607	10
CTATACC	90	7.466313E-6	11.609734	4
CGTCTTA	140	6.7848305E-10	11.536754	15
>>END_MODULE