FastQCFastQC Report
Thu 2 Feb 2017
SRR4062399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199621
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA18370.15313169742777094No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16900.14087782724710554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT1105.2441465E-922.0035651
ACGATTA1004.624235E-821.99989540
CTATGCG550.004483714219.99990738
CGATTAA1101.265671E-719.99990741
TAGGACG6350.019.0550274
TTAGGAC5000.018.4799143
AGGACGT6600.018.3332465
GGACGTG6700.018.0596166
ACCGTCG2200.017.9999168
CGTTTAT3350.017.73125831
ATTGTCG1254.8526454E-717.59991643
TAGGACC6350.017.3227524
GTGTTAG2055.456968E-1217.1735131
ATCGTTT3150.016.76182629
GATATAC2500.016.722711
TACCGTC2500.016.7199217
TGTAGGA6450.016.7137952
GTCCTAC7000.016.6598421
TTAACGG1854.2200554E-1016.64857135
GACGTGA4100.016.6340687
Produced by FastQC (version 0.11.5)