##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062399_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1199621
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19090195986899	32.0	32.0	32.0	32.0	32.0
2	31.366182319249162	32.0	32.0	32.0	32.0	32.0
3	31.46786443385036	32.0	32.0	32.0	32.0	32.0
4	31.58033245500037	32.0	32.0	32.0	32.0	32.0
5	31.508978252298018	32.0	32.0	32.0	32.0	32.0
6	35.078208867634025	36.0	36.0	36.0	36.0	36.0
7	35.09135885417144	36.0	36.0	36.0	36.0	36.0
8	35.03798699756006	36.0	36.0	36.0	36.0	36.0
9	35.15356683485868	36.0	36.0	36.0	36.0	36.0
10	35.04050779371151	36.0	36.0	36.0	36.0	36.0
11	35.17969592062826	36.0	36.0	36.0	36.0	36.0
12	35.083575562615195	36.0	36.0	36.0	36.0	36.0
13	35.131922498855886	36.0	36.0	36.0	36.0	36.0
14	35.09127716170357	36.0	36.0	36.0	36.0	36.0
15	35.05328432896723	36.0	36.0	36.0	36.0	36.0
16	35.06273648093856	36.0	36.0	36.0	36.0	36.0
17	35.040355245531714	36.0	36.0	36.0	36.0	36.0
18	35.05942460160334	36.0	36.0	36.0	36.0	36.0
19	35.02271384045461	36.0	36.0	36.0	36.0	36.0
20	35.00835013725168	36.0	36.0	36.0	36.0	36.0
21	34.98679833047271	36.0	36.0	36.0	36.0	36.0
22	34.975248849428276	36.0	36.0	36.0	36.0	36.0
23	34.9406470877052	36.0	36.0	36.0	32.0	36.0
24	34.92484543034842	36.0	36.0	36.0	32.0	36.0
25	34.89219761908136	36.0	36.0	36.0	32.0	36.0
26	34.848988138753825	36.0	36.0	36.0	32.0	36.0
27	34.83045061731997	36.0	36.0	36.0	32.0	36.0
28	34.81665876139214	36.0	36.0	36.0	32.0	36.0
29	34.777073759128925	36.0	36.0	36.0	32.0	36.0
30	34.76240079158334	36.0	36.0	36.0	32.0	36.0
31	34.74717098150166	36.0	36.0	36.0	32.0	36.0
32	34.698000451809364	36.0	36.0	36.0	32.0	36.0
33	34.673666099543105	36.0	36.0	36.0	32.0	36.0
34	34.642743833260674	36.0	36.0	36.0	32.0	36.0
35	34.598468182867755	36.0	36.0	36.0	32.0	36.0
36	34.56253433376041	36.0	36.0	36.0	32.0	36.0
37	34.536450262207815	36.0	36.0	36.0	32.0	36.0
38	34.49153440961771	36.0	36.0	36.0	32.0	36.0
39	34.45749282481717	36.0	36.0	36.0	32.0	36.0
40	34.42386387033905	36.0	36.0	36.0	32.0	36.0
41	34.39617679250363	36.0	36.0	36.0	32.0	36.0
42	34.335685187238305	36.0	36.0	36.0	32.0	36.0
43	34.33555681336022	36.0	36.0	36.0	32.0	36.0
44	34.2946597300314	36.0	36.0	36.0	32.0	36.0
45	34.21432102305645	36.0	36.0	36.0	32.0	36.0
46	34.22903150244952	36.0	36.0	36.0	32.0	36.0
47	34.172561167235315	36.0	36.0	36.0	32.0	36.0
48	34.13154071160808	36.0	36.0	36.0	32.0	36.0
49	34.115892436027714	36.0	36.0	36.0	32.0	36.0
50	33.566801514811765	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	15.0
21	42.0
22	126.0
23	343.0
24	978.0
25	2228.0
26	4627.0
27	8584.0
28	14345.0
29	21725.0
30	30719.0
31	43439.0
32	62874.0
33	99153.0
34	209701.0
35	700714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.66178277783058	18.463317476118302	11.98186841679631	25.89303132925481
2	15.565322067017684	21.177777925979775	37.51129577079672	25.74560423620582
3	18.328153863595443	25.349947649565525	28.955542143766966	27.366356343072066
4	12.017502252802354	16.353969641977397	36.601515316972	35.02701278824825
5	13.619634868012481	37.56228008679408	33.9163785895712	14.901706455622236
6	33.189648345510854	36.10533379459243	17.239781096435106	13.465236763461611
7	28.980343775528915	31.219886297327488	21.544905886864175	18.254864040279422
8	27.59138094448163	33.35995285177569	20.173954940768795	18.87471126297389
9	27.389400485653386	14.219074190931968	19.10836839301746	39.28315693039718
10	15.394195333359454	27.37572950123414	32.671151972164544	24.558923193241867
11	36.410833088116995	21.456610046006197	22.97342243925373	19.159134426623076
12	24.113782603005447	24.356609295769246	29.84317546958581	21.686432631639494
13	29.685875789103395	20.09726405256327	25.796314002505795	24.420546155827548
14	23.09079284207262	20.298327555119492	26.152759913339295	30.45811968946859
15	24.595768163444955	28.202407260293043	23.46082637766428	23.740998198597723
16	24.721891330678606	26.205443219150048	25.201959618912973	23.870705831258373
17	22.942829443632615	26.49120013737672	26.693847473493708	23.872122945496955
18	23.930141269617657	25.02206947027436	28.23416729116946	22.813621968938524
19	24.73322824458725	25.927522109066114	26.463608089554953	22.875641556791688
20	24.788495699891882	24.788829138536254	27.013281694801943	23.40939346676992
21	25.684278617996853	24.831425925354758	25.38310016246798	24.10119529418041
22	24.601686699382554	25.641931910161624	26.010965129820168	23.74541626063565
23	23.244508057128044	25.22129906028654	26.61640634833835	24.917786534247067
24	23.706070500599775	26.010798410497983	26.507538630950943	23.7755924579513
25	24.08902478366084	25.26631327727674	26.52771166893544	24.11695027012698
26	23.0377760976175	25.972953124361776	27.14198901152948	23.84728176649125
27	24.273749792642842	26.014049437280605	26.18668729540413	23.52551347467242
28	23.382051497931432	25.391519488238366	26.89307706350589	24.33335195032431
29	23.31586392702362	25.910766817186428	26.89132651062294	23.882042745167016
30	23.77976044100595	25.577911690442228	27.013365054463033	23.628962814088784
31	23.75183495453981	25.524061349376176	26.22094811611334	24.503155579970674
32	23.34262237823446	26.061147645798133	26.31356069958762	24.28266927637979
33	23.174485941809955	25.54998620397609	26.883157263835827	24.39237059037813
34	23.454157604776842	25.939775979246775	27.278532136399747	23.327534279576632
35	24.46864468027819	25.665022536284376	26.56547359541055	23.300859188026884
36	23.382801734881266	26.163429949959195	26.620490971731904	23.833277343427632
37	24.268164695349615	25.911183615491893	25.985623792847907	23.835027896310585
38	23.58911689608635	26.01029825253142	26.30005643449056	24.100528416891667
39	23.892212623820356	25.71553849090671	26.286052011426943	24.106196873845988
40	24.409792759546555	25.69278130342833	26.517458430621005	23.379967506404107
41	23.355209687059496	25.860917739852834	27.08105309927052	23.702819473817147
42	24.512741940996367	26.276632369723433	26.389334631521123	22.821291057759076
43	23.38863691115777	25.507806215463052	26.577143947963567	24.52641292541561
44	23.11746793362237	26.041391406119097	26.612655163589167	24.228485496669364
45	23.322616059572148	26.195189980835615	26.461107299722165	24.021086659870075
46	23.21526816825328	25.6369517013721	26.633850719394474	24.513929410980143
47	23.803851383061815	25.375931231613986	26.917418084545037	23.90279930077916
48	23.584282035742955	26.742112717266536	25.778141596387528	23.895463650602984
49	23.00268168029736	26.06831657665213	26.71093620401777	24.218065539032743
50	22.677468994296518	27.17606771488924	25.733941971527603	24.41252131928664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	116.0
1	107.5
2	99.0
3	334.5
4	570.0
5	563.0
6	556.0
7	482.5
8	409.0
9	547.0
10	685.0
11	1007.5
12	1330.0
13	2218.0
14	3106.0
15	3939.0
16	4772.0
17	5343.5
18	5915.0
19	6323.5
20	6732.0
21	7745.0
22	8758.0
23	9385.0
24	10012.0
25	11176.0
26	12340.0
27	14594.0
28	16848.0
29	19454.5
30	22061.0
31	24554.0
32	27047.0
33	31181.5
34	35316.0
35	40051.5
36	44787.0
37	49792.5
38	54798.0
39	56435.0
40	58072.0
41	61661.5
42	65251.0
43	66424.5
44	67598.0
45	73783.5
46	79969.0
47	84283.0
48	88597.0
49	87782.5
50	86968.0
51	81801.5
52	76635.0
53	74521.5
54	72408.0
55	72299.5
56	72191.0
57	70048.5
58	67906.0
59	61742.5
60	55579.0
61	49114.5
62	42650.0
63	37314.0
64	31978.0
65	27453.5
66	22929.0
67	20098.5
68	17268.0
69	15692.0
70	14116.0
71	11075.5
72	8035.0
73	7098.5
74	6162.0
75	4534.0
76	2906.0
77	2583.0
78	2260.0
79	1932.5
80	1605.0
81	1292.5
82	980.0
83	807.0
84	634.0
85	516.0
86	398.0
87	295.5
88	193.0
89	117.5
90	42.0
91	28.0
92	14.0
93	12.5
94	11.0
95	8.0
96	5.0
97	3.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01392106340252463
2	0.0047515006822988265
3	0.001083675594208504
4	3.334386443718474E-4
5	0.0
6	5.001579665577712E-4
7	8.335966109296185E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.335966109296185E-5
47	0.0
48	0.0
49	0.0
50	2.500789832788856E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1199621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.658768102580815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2440018101737	47.872582956162276
2	12.66199578005839	15.107981399167212
3	3.4153251455417046	6.11262272558357
4	1.2834944395057377	3.0628678850969897
5	0.6298738003234798	1.8788747493694888
6	0.3769086404412252	1.3491543105565242
7	0.25606248613425947	1.069346073603787
8	0.17466649000531764	0.8336310098015198
9	0.1185193048878767	0.6363644153386419
>10	0.6772152022251389	7.515703130471529
>50	0.0798834605270659	3.329431177363954
>100	0.07672347853306104	8.861660235592518
>500	0.004909175197624745	1.9862805876878245
>1k	4.207864455106924E-4	0.3834993442041243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1837	0.15313169742777094	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1690	0.14087782724710554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.335966109296187E-5
2	0.0	0.0	0.0	0.0	8.335966109296187E-5
3	0.0	0.0	0.0	0.0	8.335966109296187E-5
4	0.0	0.0	0.0	0.0	1.6671932218592373E-4
5	0.0	0.0	0.0	0.0	1.6671932218592373E-4
6	0.0	0.0	0.0	0.0	1.6671932218592373E-4
7	0.0	0.0	0.0	0.0	1.6671932218592373E-4
8	0.0	0.0	0.0	0.0	1.6671932218592373E-4
9	0.0	0.0	0.0	0.0	1.6671932218592373E-4
10	0.0	0.0	0.0	8.335966109296187E-5	2.500789832788856E-4
11	0.0	0.0	0.0	8.335966109296187E-5	2.500789832788856E-4
12	0.0	0.0	0.0	1.6671932218592373E-4	3.3343864437184747E-4
13	0.0	0.0	0.0	1.6671932218592373E-4	5.83517627650733E-4
14	0.0	0.0	0.0	3.3343864437184747E-4	5.83517627650733E-4
15	0.0	0.0	0.0	5.001579665577712E-4	5.83517627650733E-4
16	0.0	0.0	0.0	5.001579665577712E-4	5.83517627650733E-4
17	0.0	0.0	0.0	5.001579665577712E-4	5.83517627650733E-4
18	0.0	0.0	0.0	5.83517627650733E-4	8.335966109296186E-4
19	0.0	0.0	0.0	6.668772887436949E-4	8.335966109296186E-4
20	0.0	0.0	0.0	7.502369498366567E-4	0.0010003159331155424
21	0.0	0.0	0.0	0.001250394916394428	0.0010003159331155424
22	0.0	0.0	0.0	0.002834228477160703	0.0010003159331155424
23	0.0	0.0	0.0	0.005001579665577712	0.001083675594208504
24	0.0	0.0	0.0	0.008669404753668033	0.001083675594208504
25	0.0	0.0	0.0	0.011503633230828737	0.001083675594208504
26	0.0	0.0	0.0	0.015921695268755715	0.0013337545774873899
27	0.0	0.0	0.0	0.020673195951054543	0.0013337545774873899
28	1.6671932218592373E-4	0.0	0.0	0.029259241043629612	0.0013337545774873899
29	1.6671932218592373E-4	0.0	0.0	0.04584781360112902	0.0013337545774873899
30	1.6671932218592373E-4	0.0	0.0	0.07769120413864046	0.0013337545774873899
31	1.6671932218592373E-4	0.0	0.0	0.13821031809213077	0.0014171142385803516
32	1.6671932218592373E-4	0.0	0.0	0.21165017951503015	0.0015838335607662754
33	1.6671932218592373E-4	0.0	0.0	0.2859236375488592	0.0015838335607662754
34	1.6671932218592373E-4	0.0	0.0	0.36444843829842927	0.0016671932218592371
35	1.6671932218592373E-4	0.0	0.0	0.4581446973669184	0.0016671932218592371
36	1.6671932218592373E-4	0.0	0.0	0.5879356896886601	0.0016671932218592371
37	1.6671932218592373E-4	0.0	0.0	0.7829972966461908	0.0016671932218592371
38	1.6671932218592373E-4	0.0	0.0	1.029408454836986	0.001750552882952199
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAT	110	5.2441465E-9	22.003565	1
ACGATTA	100	4.624235E-8	21.999895	40
CTATGCG	55	0.0044837142	19.999907	38
CGATTAA	110	1.265671E-7	19.999907	41
TAGGACG	635	0.0	19.055027	4
TTAGGAC	500	0.0	18.479914	3
AGGACGT	660	0.0	18.333246	5
GGACGTG	670	0.0	18.059616	6
ACCGTCG	220	0.0	17.999916	8
CGTTTAT	335	0.0	17.731258	31
ATTGTCG	125	4.8526454E-7	17.599916	43
TAGGACC	635	0.0	17.322752	4
GTGTTAG	205	5.456968E-12	17.173513	1
ATCGTTT	315	0.0	16.761826	29
GATATAC	250	0.0	16.72271	1
TACCGTC	250	0.0	16.719921	7
TGTAGGA	645	0.0	16.713795	2
GTCCTAC	700	0.0	16.659842	1
TTAACGG	185	4.2200554E-10	16.648571	35
GACGTGA	410	0.0	16.634068	7
>>END_MODULE